Construct: ORF TRCN0000481472

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004325.1_s317c1
Derived from:
ccsbBroadEn_16162
DNA Barcode:
TCATAGCGTGATGCCATTGCCAGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATP9B (374868)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481472
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 374868 ATP9B ATPase phospholipid transpo... XM_017025742.1 19.5% 19.5% 1_159del;562_582del;589_2088del
2 human 374868 ATP9B ATPase phospholipid transpo... XM_017025737.1 13.1% 13.1% 1_159del;562_582del;589_3099del
3 human 374868 ATP9B ATPase phospholipid transpo... XM_017025736.2 13% 13% 1_159del;562_582del;589_3135del
4 human 374868 ATP9B ATPase phospholipid transpo... XM_017025735.2 12.8% 12.8% 1_159del;562_582del;589_3177del
5 human 374868 ATP9B ATPase phospholipid transpo... XM_017025734.1 12.7% 12.7% 1_159del;562_582del;589_3201del
6 human 374868 ATP9B ATPase phospholipid transpo... XM_017025733.1 12.4% 12.4% 1_159del;562_582del;589_3288del
7 human 374868 ATP9B ATPase phospholipid transpo... XM_017025732.1 12.2% 12.2% 1_159del;562_582del;589_3321del
8 human 374868 ATP9B ATPase phospholipid transpo... XM_017025731.1 12% 12% 1_159del;562_582del;589_3399del
9 human 374868 ATP9B ATPase phospholipid transpo... XM_011525965.2 11.9% 11.9% 1_39del;442_462del;469_3402del
10 human 374868 ATP9B ATPase phospholipid transpo... XM_011525966.2 11.9% 11.9% 1_39del;442_462del;469_3402del
11 human 374868 ATP9B ATPase phospholipid transpo... NM_001306085.2 11.9% 11.9% 1_156del;559_579del;586_3408del
12 human 374868 ATP9B ATPase phospholipid transpo... XM_017025730.1 11.9% 11.9% 1_159del;562_582del;589_3411del
13 human 374868 ATP9B ATPase phospholipid transpo... XM_017025729.1 11.8% 11.8% 1_159del;562_582del;589_3435del
14 human 374868 ATP9B ATPase phospholipid transpo... NM_198531.5 11.8% 11.8% 1_156del;559_579del;586_3441del
15 human 374868 ATP9B ATPase phospholipid transpo... XM_017025728.2 11.8% 11.8% 1_159del;562_582del;589_3444del
16 human 374868 ATP9B ATPase phospholipid transpo... XM_017025727.1 11.6% 11.6% 1_159del;562_582del;589_3489del
17 human 374868 ATP9B ATPase phospholipid transpo... XM_017025726.1 11.6% 11.6% 1_159del;562_582del;589_3507del
18 human 374868 ATP9B ATPase phospholipid transpo... XM_011525964.2 11.5% 11.5% 1_156del;559_579del;586_3519del
19 human 374868 ATP9B ATPase phospholipid transpo... XM_011525963.2 11.5% 11.5% 1_159del;562_582del;589_3522del
20 human 374868 ATP9B ATPase phospholipid transpo... XR_001753192.1 11.2% 1_458del;861_881del;888_3629del
21 human 374868 ATP9B ATPase phospholipid transpo... XR_001753198.1 10.7% 1_173del;576_596del;603_3785del
22 human 374868 ATP9B ATPase phospholipid transpo... XR_001753195.2 10.6% 1_161del;564_584del;591_3816del
23 human 374868 ATP9B ATPase phospholipid transpo... XR_002958170.1 10.3% 1_161del;564_584del;591_3932del
24 human 374868 ATP9B ATPase phospholipid transpo... XR_002958169.1 10% 1_161del;564_584del;591_4049del
25 human 374868 ATP9B ATPase phospholipid transpo... XR_001753194.1 8.6% 1_458del;861_881del;888_4734del
26 human 374868 ATP9B ATPase phospholipid transpo... XR_001753193.1 8.5% 1_458del;861_881del;888_4767del
27 human 374868 ATP9B ATPase phospholipid transpo... NR_148360.2 8.4% 1_173del;576_596del;603_4828del
28 mouse 50771 Atp9b ATPase, class II, type 9B NM_015805.3 10.9% 11.1% (many diffs)
29 mouse 50771 Atp9b ATPase, class II, type 9B NM_001201569.1 10.8% 10.9% (many diffs)
30 mouse 50771 Atp9b ATPase, class II, type 9B XM_006526507.2 10.7% 10.8% (many diffs)
31 mouse 50771 Atp9b ATPase, class II, type 9B XM_006526506.2 10.6% 10.7% (many diffs)
32 mouse 50771 Atp9b ATPase, class II, type 9B XR_001782382.1 8.1% (many diffs)
33 mouse 50771 Atp9b ATPase, class II, type 9B XR_001782381.1 8.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
474
ORF length:
408
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc actaatgatg tctgaagaag gctttgagaa tgaggaaagt gattaccaca
121ccttaccacg agCCAGGATA ATGCAAAGGA AAAGAGGACT GGAGTGGTTT GTCTGTGATG
181GCTGGAAGTT CCTCTGTACC AGTTGCTGTG GTTGGCTGAT AAATATTTGT CGAAGAAAGA
241AAGAGCTGAA AGCTCGCACA GTATGGCTTG GATGTCCTGA AAAGTGTGAA GAAAAACATC
301CCAGGAATTC TATAAAAAAT CAAAAATACA ATGTGTTTAC CTTTATACCT GGGGTTTTGT
361ATGAACAATT CAAGTTTTTC TTGAATCTCT ATTTTCTAGT AATATCCTGC TCACAGTTTG
421TACCAGCATT GAAAATAGGC TATCTCTACA CCTACTGGGC TCCTCTGATG ACATGCCCAA
481CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
541ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
601TATCTTGTGG AAAGGACGAT CATAGCGTGA TGCCATTGCC AGAACGCGTT AAGTCgacaa
661tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)