Construct: ORF TRCN0000481477
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016854.2_s317c1
- Derived from:
- ccsbBroadEn_12754
- DNA Barcode:
- CGGTGAATTACTTTTATCTATGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG10 (83734)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481477
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83734 | ATG10 | autophagy related 10 | NM_001131028.2 | 97.8% | 85.2% | (many diffs) |
| 2 | human | 83734 | ATG10 | autophagy related 10 | NM_031482.5 | 97.8% | 85.2% | (many diffs) |
| 3 | human | 83734 | ATG10 | autophagy related 10 | XM_005248610.5 | 97.8% | 85.2% | (many diffs) |
| 4 | human | 83734 | ATG10 | autophagy related 10 | XM_005248611.5 | 97.8% | 85.2% | (many diffs) |
| 5 | human | 83734 | ATG10 | autophagy related 10 | XM_011543660.2 | 82% | 81.4% | 0_1ins114;509C>T;533C>A |
| 6 | human | 83734 | ATG10 | autophagy related 10 | XM_005248612.3 | 81.5% | 69.5% | (many diffs) |
| 7 | human | 83734 | ATG10 | autophagy related 10 | XM_017009944.1 | 81.5% | 69.5% | (many diffs) |
| 8 | human | 83734 | ATG10 | autophagy related 10 | XM_011543661.2 | 68.8% | 68% | (many diffs) |
| 9 | mouse | 66795 | Atg10 | autophagy related 10 | XM_011244551.2 | 68.9% | 62.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 714
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ttcattggag aaaaaacatt ccaacgttat tgtgcagaat tcattaaaca 121 ttcacaacat aggtgatagt tgggaatgga gaccatcaaa ggactgttct gatggctaca 181 tgtgcaaaat acactttcaa attaagaatg ggtctgtgat gtcacatcta ggagcatcta 241 cccatggaca gacatgtctt cccatggagg aggCTTTCGA GCTACCCTTG GATGATTGTG 301 AAGTGATTGA AACTGCAGCA GCGTCCGAAG TGATTAAATA TGAGTATCAT GTCTTATATT 361 CCTGTAGCTA CCAAGTGCCT GTACTTTACT TTAGGGCAAG CTTTTTAGAT GGGAGACCTT 421 TAACTCTGAA GGACATATGG GAAGGAGTTC ATGAGTGCTA TAAGATGCGA CTGCTACAGG 481 GACCATGGGA CACTATTACG CAACAGGAAC ATCCAATACT TGGGCAACCC TTTTTTGTAC 541 TTCATCCCTG CAAGACGAAT GAATTCATGA CTCCTGTATT AAAGAATTCT CAGAAAATCA 601 ATAAGAATGT CAACTATATC ACATCATGGC TGAGCATTGT AGGGCCAGTT GTTGGGCTGA 661 ATCTACCTCT GAGTTATGCC AAAGCAATGT CTCAGGATGA ACGAAATGTC CATTACCCAA 721 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 781 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 841 TATCTTGTGG AAAGGACGAC GGTGAATTAC TTTTATCTAT GGAACGCGTT AAGTCgacaa 901 tcaacctctg gattacaaaa tttgtgaaag att