Construct: ORF TRCN0000481483
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013746.1_s317c1
- Derived from:
- ccsbBroadEn_08350
- DNA Barcode:
- CATGCGCTACTTAATTGAAGTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCO1 (53630)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481483
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53630 | BCO1 | beta-carotene oxygenase 1 | NM_017429.3 | 99.8% | 99.8% | 105T>C;801A>T |
| 2 | human | 53630 | BCO1 | beta-carotene oxygenase 1 | XM_011523109.2 | 87.6% | 87.5% | 105T>C;801A>T;1097_1098ins201 |
| 3 | human | 53630 | BCO1 | beta-carotene oxygenase 1 | XM_017023286.2 | 87.2% | 87.2% | 105T>C;801A>T;1206_1207ins207 |
| 4 | human | 53630 | BCO1 | beta-carotene oxygenase 1 | XM_017023287.2 | 79.2% | 79.1% | (many diffs) |
| 5 | human | 53630 | BCO1 | beta-carotene oxygenase 1 | XM_017023288.2 | 74% | 73.3% | (many diffs) |
| 6 | human | 53630 | BCO1 | beta-carotene oxygenase 1 | XR_002957813.1 | 60.1% | (many diffs) | |
| 7 | human | 53630 | BCO1 | beta-carotene oxygenase 1 | XM_017023289.1 | 52.5% | 52.4% | 0_1ins777;24A>T |
| 8 | mouse | 63857 | Bco1 | beta-carotene oxygenase 1 | NM_021486.3 | 81% | 82.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1710
- ORF length:
- 1641
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatataata tttggcagga ataggaaaga acagctggag cctgtgaggg 121 ccaaagtgac aggcaagatt ccagcatggc tgcagggaac cctgctccgc aacgggcctg 181 ggatgcacac agttggggag tccagataca accattggtt cgacggcctt gccctgctcc 241 acagcttcac catcagagac ggtgaagtct attacaggag caaatacctg agaagcgata 301 cctacaacac caatattgag gcaaacagga ttgtggtgtc tgagtttgga acaatggcct 361 atccggaccc ctgcaaaaac atattttcca aagctttctc ctacttgtct cacaccatcc 421 ccgatttcac cgacaactgc ctgatcaaca tcatgaagtg cggagaagac ttctacgcga 481 cctcagagac caattacatc aggaaaatca acccacagac tctggaaacc ctggagaagg 541 ttgattatcg taaatacgtg gcggtaaatc tggcaacgtc acatccccat tatgatgagg 601 ctggaaatgt tctaaacatg ggcacatcca ttgtggaaaa ggggaagaca aagtatgtga 661 tttttaagat ccctgccaca gtaccagagg gcaagaagca ggggaagagc ccctggaagc 721 acacagaggt gttctgctcc atcccatccc gctccctgct ctccccaagc tactaccaca 781 gctttggagt caccgagaac tatgtcatct tccttgagca gcctttcagg ttggatattc 841 tcaagatggc aaccgcatac atccggagta tgagctgggc ctcctgcctg gctttccaca 901 gggaggagaa gacttatatc cacatcatcg accaaaggac caggcagcct gtgcagacca 961 agttttacac agacgccatg gtggtcttcc atcacgtcaa cgcctacgaa gaggacggct 1021 gcatcgtgtt tgacgtcatt gcctacgagg acaacagcct ctaccagctc ttctacctgg 1081 ccaacctgaa ccaggacttc aaggagaact ccaggctcac ctcggtcccc accctcagga 1141 ggtttgccgt gcccctccac gtggacaaga atgcagaagt gggcacaaat ttaatcaaag 1201 tggcatctac aacagccacg gccctGAAGG AAGAAGATGG CCAAGTCTAC TGCCAGCCGG 1261 AATTTCTTTA TGAAGGCTTA GAGCTTCCAC GGGTCAATTA TGCTCACAAT GGAAAGCAAT 1321 ACCGATATGT CTTTGCTACA GGAGTTCAGT GGAGTCCAAT CCCAACCAAG ATAATAAAAT 1381 ATGACATTCT CACAAAGTCA TCCTTAAAAT GGAGAGAGGA CGACTGCTGG CCAGCGGAAC 1441 CCCTGTTTGT GCCCGCGCCA GGTGCCAAGG ATGAGGATGA CGGAGTAATC TTATCAGCCA 1501 TTGTCTCTAC TGATCCCCAA AAGCTGCCTT TTCTGCTCAT TCTGGATGCC AAAAGCTTTA 1561 CGGAATTGGC CCGTGCCTCT GTTGATGTCG ATATGCACAT GGATCTCCAT GGATTATTCA 1621 TTACAGACAT GGACTGGGAC ACAAAAAAGC AGGCCGCTTC TGAGGAACAG CGGGACAGGG 1681 CTTCCGACTG CCACGGGGCT CCTCTGACCT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1741 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1801 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACATGC 1861 GCTACTTAAT TGAAGTGCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1921 tgaaagatt