Construct: ORF TRCN0000481486
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011642.1_s317c1
- Derived from:
- ccsbBroadEn_11368
- DNA Barcode:
- CTGCCTACAAACCATCTCCCTGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGEF6 (9459)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481486
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9459 | ARHGEF6 | Rac/Cdc42 guanine nucleotid... | NM_001306177.2 | 86.4% | 86.4% | 1615_1866del |
| 2 | human | 9459 | ARHGEF6 | Rac/Cdc42 guanine nucleotid... | XM_011531416.3 | 82.8% | 82.7% | 200_280del;1696_1947del |
| 3 | human | 9459 | ARHGEF6 | Rac/Cdc42 guanine nucleotid... | XM_011531415.3 | 72.1% | 71.9% | 1_291del;491_571del;1987_2238del |
| 4 | human | 9459 | ARHGEF6 | Rac/Cdc42 guanine nucleotid... | NM_004840.3 | 69.3% | 69.3% | 1_462del;2077_2328del |
| 5 | human | 9459 | ARHGEF6 | Rac/Cdc42 guanine nucleotid... | XM_011531413.2 | 68.9% | 68.9% | 1_462del;1705_1716del;2089_2340del |
| 6 | human | 9459 | ARHGEF6 | Rac/Cdc42 guanine nucleotid... | XM_011531414.2 | 68% | 68% | (many diffs) |
| 7 | human | 9459 | ARHGEF6 | Rac/Cdc42 guanine nucleotid... | XM_011531412.3 | 66.9% | 66.8% | 1_462del;662_742del;2158_2409del |
| 8 | human | 9459 | ARHGEF6 | Rac/Cdc42 guanine nucleotid... | XM_017029975.2 | 66.6% | 66.5% | (many diffs) |
| 9 | human | 9459 | ARHGEF6 | Rac/Cdc42 guanine nucleotid... | XM_005262499.3 | 63% | 63% | 1_462del;1704_1705ins147;1930_2181del |
| 10 | mouse | 73341 | Arhgef6 | Rac/Cdc42 guanine nucleotid... | XM_006528316.3 | 77.5% | 80.5% | (many diffs) |
| 11 | mouse | 73341 | Arhgef6 | Rac/Cdc42 guanine nucleotid... | NM_152801.2 | 60.3% | 62.7% | (many diffs) |
| 12 | mouse | 73341 | Arhgef6 | Rac/Cdc42 guanine nucleotid... | XM_006528313.3 | 59.4% | 61.8% | (many diffs) |
| 13 | mouse | 73341 | Arhgef6 | Rac/Cdc42 guanine nucleotid... | XM_006528314.3 | 55.1% | 57.4% | (many diffs) |
| 14 | mouse | 73341 | Arhgef6 | Rac/Cdc42 guanine nucleotid... | XM_006528315.3 | 54.2% | 56.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1680
- ORF length:
- 1614
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ggaaaatgga agtcatcagt tgatagtaaa agcaagattc aactttaagc 121 agactaatga ggatgaactg tcagtttgta agggggacat catttacgtc acacgagttg 181 aagaaggagg ctggtgggaa ggcacattaa atgggagaac aggctggttc cccagtaatt 241 atgtccgtga aattaaatcc agtgagagac ctctctcccc aaaagccgtc aaaggatttg 301 aaactgctcc acttaccaag aattattata ctgtggtgtt acagaacatc ctggacactg 361 aaaaagaata tgctaaagaa cttcagtctc ttcttgttac ttacttaaga cccctgcagt 421 ccaataacaa tctgagtact gtggaggtta catctttact gggaaacttc gaggaagtat 481 gcacatttca acagacactc tgccaagcct tggaagaatg ttcaaagttt ccagaaaacc 541 agcacaaagt aggaggttgt ctactgagtc tcatgcctca ttttaaatct atgtatctgg 601 cttactgtgc aaaccatcct tcagctgtaa atgtgctcac tcagcacagt gatgagttgg 661 aacaattcat ggaaaatcaa ggtgcatcga gcccaggtat cctcatttta acaacaaacc 721 tcagcaaacc attcatgcga ctggagaaat atgttactct cttgcaagag ttagaacggc 781 atatggagga tactcatcca gatcatcagg atattctgaa agcaatcgta gcattcaaaa 841 ctctcatggg gcaatgtcaa gatctgagga agagaaaaca gctggagtta cagatactgt 901 ccgaacctat tcaggcatgg gaaggagaag atattaaaaa cttgggaaat gtgattttta 961 tgtcacaagt aatggtgcag tatggagcat gtgaggaaaa agaggagcgg taccttatgt 1021 tattttcaaa tgtcctgata atgttatctg caagtcctcg gatgagtggc tttatctatc 1081 agggaaaaat accaatagca ggaacggtgg tgactagatt agatgaaatt gaagggaatg 1141 actgcacatt tgaaatcact ggtaacacag tggagagaat tgtggtccat tgtaacaaca 1201 aCCAGGACTT CCAGGAATGG TTGGAGCAGC TGAACAGACT GATCAGAGGA CCTGCCTCTT 1261 GCAGTTCATT ATCCAAAACC TCATCGTCAT CATGTAGTGC TCATTCTTCT TTTAGCTCTA 1321 CCGGACAGCC CCGAGGACCC TTGGAGCCTC CTCAAATTAT AAAACCGTGG AGTTTAAGTT 1381 GTCTACGACC TGCACCTCCA CTTAGACCAT CAGCAGCACT AGGTTATAAA GAGAGGATGT 1441 CTTATATCTT AAAGGAGTCT AGTAAAAGCC CTAAAACGAT GAAGAAATTT CTTCATAAAA 1501 GGAAGACTGA GAGAAAACCA TCGGAGGAGG AATATGTGAT TAGGAAAAGT ACAGCTGCTC 1561 TGGAAGAGGA TGCTCAAATC CTTAAAGTGA TCGAAGCCTA CTGCACCAGC GCAAATTTTC 1621 AACAAGGCCA TGGCTCAAGT ACTCGAAAAG ATTCCATTCC ACAAGTCCTA CTCCCTGAGT 1681 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1741 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1801 TTTATATATC TTGTGGAAAG GACGACTGCC TACAAACCAT CTCCCTGGAA CGCGTTAAGT 1861 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt