Construct: ORF TRCN0000481511

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009009.1_s317c1
Derived from:
ccsbBroadEn_11098
DNA Barcode:
AACTACTTAATTAAATATAGCAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RFX3 (5991)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481511
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5991 RFX3 regulatory factor X3 NM_001282117.1 99.8% 99.7% 929A>G;1011A>G
2 human 5991 RFX3 regulatory factor X3 NM_002919.3 57.8% 56.1% (many diffs)
3 human 5991 RFX3 regulatory factor X3 NM_001282116.1 54.5% 53% (many diffs)
4 human 5991 RFX3 regulatory factor X3 NM_134428.3 54.5% 53% (many diffs)
5 human 5991 RFX3 regulatory factor X3 XM_006716843.2 53% 51.6% (many diffs)
6 human 5991 RFX3 regulatory factor X3 XM_006716844.2 53% 51.6% (many diffs)
7 human 5991 RFX3 regulatory factor X3 XM_006716845.2 53% 51.6% (many diffs)
8 human 5991 RFX3 regulatory factor X3 XM_011517998.2 53% 51.6% (many diffs)
9 human 5991 RFX3 regulatory factor X3 XM_011517999.2 53% 51.6% (many diffs)
10 human 5991 RFX3 regulatory factor X3 XM_017015000.2 53% 51.6% (many diffs)
11 human 5991 RFX3 regulatory factor X3 XM_024447634.1 53% 51.6% (many diffs)
12 human 5991 RFX3 regulatory factor X3 XM_024447635.1 53% 51.6% (many diffs)
13 human 5991 RFX3 regulatory factor X3 XM_024447632.1 52.6% 51.2% (many diffs)
14 human 5991 RFX3 regulatory factor X3 XM_006716840.2 52.4% 50.9% (many diffs)
15 human 5991 RFX3 regulatory factor X3 XM_017015001.1 51.2% 49.7% (many diffs)
16 human 5991 RFX3 regulatory factor X3 XM_024447630.1 51.1% 49.6% (many diffs)
17 human 5991 RFX3 regulatory factor X3 XM_024447631.1 51.1% 49.6% (many diffs)
18 human 5991 RFX3 regulatory factor X3 XM_024447633.1 49.8% 48.3% (many diffs)
19 human 5991 RFX3 regulatory factor X3 XM_011518001.1 42.3% 40.9% (many diffs)
20 human 5991 RFX3 regulatory factor X3 XM_017015002.2 17.7% 14.9% (many diffs)
21 human 5991 RFX3 regulatory factor X3 XM_006716847.3 16.8% 15.2% (many diffs)
22 mouse 19726 Rfx3 regulatory factor X, 3 (inf... NM_001166414.1 50.8% 52.9% (many diffs)
23 mouse 19726 Rfx3 regulatory factor X, 3 (inf... NM_011265.3 50.8% 52.9% (many diffs)
24 mouse 19726 Rfx3 regulatory factor X, 3 (inf... XM_006526782.3 50.8% 52.9% (many diffs)
25 mouse 19726 Rfx3 regulatory factor X, 3 (inf... XM_011247169.2 50.8% 52.9% (many diffs)
26 mouse 19726 Rfx3 regulatory factor X, 3 (inf... XM_006526780.3 50.1% 52.2% (many diffs)
27 mouse 19726 Rfx3 regulatory factor X, 3 (inf... XM_006526778.3 49% 51.1% (many diffs)
28 mouse 19726 Rfx3 regulatory factor X, 3 (inf... XM_011247167.2 49% 51.1% (many diffs)
29 mouse 19726 Rfx3 regulatory factor X, 3 (inf... XM_017318087.1 48.7% 50.8% (many diffs)
30 mouse 19726 Rfx3 regulatory factor X, 3 (inf... XM_017318086.1 48.1% 50.1% (many diffs)
31 mouse 19726 Rfx3 regulatory factor X, 3 (inf... XM_006526784.3 47.5% 49.6% (many diffs)
32 mouse 19726 Rfx3 regulatory factor X, 3 (inf... XM_011247168.2 45.9% 47.9% (many diffs)
33 mouse 19726 Rfx3 regulatory factor X, 3 (inf... XM_006526785.3 22.3% 22% (many diffs)
34 mouse 19726 Rfx3 regulatory factor X, 3 (inf... XR_877668.2 14% (many diffs)
35 mouse 19726 Rfx3 regulatory factor X, 3 (inf... XR_001782562.1 12.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1305
ORF length:
1239
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca gacatcagag actgggtcgg acacaggctc gacagtgacc ttacaaacat
121ctgtggctag tcaagcagca gtgcctacgc aggtggtaca gcaagtacca gtacaacaac
181aggtacagca ggtacagact gtgcagcagg tacaacatgt ctatcccgct caggtgcagt
241atgtggaagg aagcgatact gtctatacca atggagcaat ccgaacaaca acgtatcctt
301acacagagac acagatgtac agccaaaata ctggagggaa ttactttgat actcaaggga
361gttccgccca ggtgactacc gtggtctcat cccacagtat ggtgggcact ggtgggattc
421agatgggcgt cacaggagga caactcatca gcagctctgg aggaacctat ctgatcggca
481actcaatgga gaattctggt cactcagtga cacacacaac tcgggcctcc ccagcgacaa
541ttgaaatggc gattgagacg ctgcaaaagt ctgacggtct gtccactcac agaagctctc
601ttctcaacag ccatctccag tggctgttgg acaattatga gacagcagaa ggagtgagcc
661ttcccagaag cactctgtac aaccactacc ttcgacactg tcaggaacac aaactggacc
721cagtcaatgc tgcctctttt ggaaaattaa taagatcaat ttttatgggg ctacgaacca
781ggagattggg cactagagga aactccaaat accactacta tgggattcgt gtcaagccag
841attcccctct taatcgTCTG CAAGAAGACA TGCAGTATAT GGCTATGAGA CAACAACCCA
901TGCAACAGAA ACAAAGGTAC AAGCCTATGC AGAAAGTGGA TGGGGTTGCA GATGGTTTCA
961CAGGAAGTGG TCAACAGACA GGCACATCTG TTGGGCAAAC TGTAATTGCC CAAAGCCAAC
1021ATCATCAACA GTTTTTAGAT GCATCTCGAG CACTTCCAGA GTTTGGAGAA GTTGAGATCT
1081CTTCTCTGCC AGATGGTACT ACCTTTGAGG ATATCAAGTC ACTGCAGAGT CTTTATAGAG
1141AGCACTGTGA GGCAATATTG GACGTTGTTG TGAATCTTCA ATTTAGCCTG ATAGAAAAAT
1201TGTGGCAAAC ATTCTGGCGC TATTCTCCCT CTACTCCAAC TGATGGCACT ACCATTACCG
1261AATCGAGGTC AGAATCAACA TCTTTTCCAA TTCATTTTCA TGGCTGCCCA ACTTTCTTGT
1321ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1381AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1441GAAAGGACGA AACTACTTAA TTAAATATAG CAACACGCGT TAAGTCgaca atcaacctct
1501ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)