Construct: ORF TRCN0000481515
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004094.1_s317c1
- Derived from:
- ccsbBroadEn_11486
- DNA Barcode:
- ACTGCCGGCGACGTACGGTTCCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRDN (10345)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481515
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10345 | TRDN | triadin | NM_001256022.1 | 99.8% | 99.4% | 383C>G |
| 2 | human | 10345 | TRDN | triadin | NM_001256021.1 | 56.5% | 56.4% | (many diffs) |
| 3 | human | 10345 | TRDN | triadin | NM_001256020.2 | 54.5% | 54.3% | (many diffs) |
| 4 | human | 10345 | TRDN | triadin | NM_001251987.2 | 35.2% | 35% | (many diffs) |
| 5 | human | 10345 | TRDN | triadin | NM_006073.4 | 22.3% | 22.1% | (many diffs) |
| 6 | mouse | 76757 | Trdn | triadin | NM_029726.2 | 20.9% | 20.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 567
- ORF length:
- 501
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggCATGAC TGAGATCACT GCTGAAGGAA ATGCATCTAC AACCACAACT GTGATAGACA 121 GCAAAAATGG ATCTGTGCCC AAATCCCCCG GAAAAGTGCT GAAGAGGACA GTCACAGAAG 181 ACATAGTGAC GACGTTCAGC TCCCCTGCAG CCTGGCTTCT GGTCATTGCC CTGATAATCA 241 CGTGGTCAGC TGTTGCCATC GTTATGTTTG ATTTAGTGGA TTACAAAAAC TTTTCAGCAA 301 GCTCTATTGC CAAGATTGGC TCAGATCCTT TAAAACTGGT ACGTGATGCT ATGGAGGAAA 361 CCACGGACTG GATCTATGGC TTCTTTTCTT TGTTATCTGA CATCATCTCA TCTGAAGATG 421 AAGAAGATGA TGATGGTGAC GAAGATAGTG ATAAAGGAGA AATAGATGAG CCTCCCTTGA 481 GAAAAAAAGA AATACACAAA GATAAGACTG AAAAACAAGA GAAACCTGAA AGGAAAATAC 541 AAACTAAAGA AGTTGGACAC AGCTCTTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 601 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 661 AACTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AACTGCCGGC 721 GACGTACGGT TCCAGACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 781 agatt