Construct: ORF TRCN0000481546
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011391.2_s317c1
- Derived from:
- ccsbBroadEn_14737
- DNA Barcode:
- CCGGTGAGACCGACCATTCAGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDK1 (5163)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481546
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | NM_002610.5 | 100% | 100% | |
| 2 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | NM_001278549.2 | 95.6% | 95.3% | 411_470del |
| 3 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XM_011511345.3 | 82.5% | 82.5% | 0_1ins228 |
| 4 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XM_011511343.2 | 78.9% | 78.7% | 0_1ins228;183_242del |
| 5 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XM_011511344.2 | 78.9% | 78.7% | 0_1ins228;183_242del |
| 6 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XM_011511347.2 | 78.9% | 78.7% | 0_1ins228;183_242del |
| 7 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_427094.1 | 78.1% | (many diffs) | |
| 8 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_922943.2 | 77.5% | 1_61del;472_531del;1430_1686del | |
| 9 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_001738772.2 | 75.6% | 1_61del;472_531del;1430_1729del | |
| 10 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_001738775.2 | 74.4% | 1_73del;1382_1758del | |
| 11 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_922945.1 | 72.4% | 1_61del;472_531del;1430_1806del | |
| 12 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_001738773.1 | 71.9% | 1_61del;472_531del;1430_1819del | |
| 13 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_922942.2 | 69.6% | 1_61del;472_531del;1430_1877del | |
| 14 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XM_006712594.2 | 56.8% | 55.7% | (many diffs) |
| 15 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XM_006712595.3 | 55.6% | 54.8% | (many diffs) |
| 16 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | NR_103731.1 | 54.5% | (many diffs) | |
| 17 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | NR_103729.2 | 44.3% | 1_61del;1370_2952del | |
| 18 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_427093.3 | 43.4% | 1_61del;472_531del;1430_3008del | |
| 19 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_001738776.1 | 40.9% | (many diffs) | |
| 20 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_001738774.2 | 39.1% | 1_73del;1382_3337del | |
| 21 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_922944.2 | 38.6% | 1_61del;472_531del;1430_3385del | |
| 22 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_001738771.1 | 36.1% | 1_61del;472_531del;1430_3619del | |
| 23 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_427095.3 | 28.9% | 1_73del;841_842ins77;1305_4168del | |
| 24 | mouse | 228026 | Pdk1 | pyruvate dehydrogenase kina... | NM_172665.5 | 85.6% | 91.5% | (many diffs) |
| 25 | mouse | 228026 | Pdk1 | pyruvate dehydrogenase kina... | XM_011239444.1 | 79% | 84.3% | (many diffs) |
| 26 | mouse | 228026 | Pdk1 | pyruvate dehydrogenase kina... | XM_017317590.1 | 72.4% | 78.6% | (many diffs) |
| 27 | mouse | 228026 | Pdk1 | pyruvate dehydrogenase kina... | XM_011239445.2 | 66.8% | 72.5% | (many diffs) |
| 28 | mouse | 228026 | Pdk1 | pyruvate dehydrogenase kina... | XM_011239446.2 | 66.8% | 72.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1374
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gctggcgcgg ctgcttcgcg gagccgcctt ggccggcccg ggcccggggc 121 tgcgcgccgc cggcttcagc cgcagcttca gctcggactc gggctccagc ccggcgtccg 181 agcgcggcgt tccgggccag gtggacttct acgcgcgctt ctcgccgtcc ccgctctcca 241 tgaagcagtt cctggacttc ggatcagtga atgcttgtga aaagacctca tttatgtttc 301 tgcggcaaga gttgcctgtc agactggcaa atataatgaa agaaataagt ctccttccag 361 ataatcttct caggacacca tccgttcaat tggtacaaag ctggtatatc cagagtcttc 421 aggagcttct tgattttaag gacaaaagtg ctgaggatgc taaagctatt tatgacttta 481 cagatactgt gatacggatc agaaaccgac acaatgatgt cattcccaca atggcccagg 541 gtgtgattga atacaaggag agctttgggg tggatcctgt caccagccag aatgttcagt 601 actttttgga tcgattctac atgagtcgca tttcaattag aatgttactc aatcagcact 661 ctttattgtt tggtggaaaa ggcaaaggaa gtccatctca tcgaaaacac attggaagca 721 taaatccaaa ctgcaatgta cttgaagtta ttaaagatgg ctatgaaaat gctaggcgtc 781 tgtgtgattt gtattatatt aactctcccg aactagaact tgaagaacta aatgcaaaat 841 caccaggaca gccaatacaa gtggtttatg taccatccca tctctatcac atggtgtttg 901 aacttttcaa gaatgcaatg agagccacta tggaaCACCA TGCCAACAGA GGTGTTTACC 961 CCCCTATTCA AGTTCATGTC ACGCTGGGTA ATGAGGATTT GACTGTGAAG ATGAGTGACC 1021 GAGGAGGTGG CGTTCCTTTG AGGAAAATTG ACAGACTTTT CAACTACATG TATTCAACTG 1081 CACCAAGACC TCGTGTTGAG ACCTCCCGCG CAGTGCCTCT GGCTGGTTTT GGTTATGGAT 1141 TGCCCATATC ACGTCTTTAC GCACAATACT TCCAAGGAGA CCTGAAGCTG TATTCCCTAG 1201 AGGGTTACGG GACAGATGCA GTTATCTACA TTAAGGCTCT GTCAACAGAC TCAATAGAAA 1261 GACTCCCAGT GTATAACAAA GCTGCCTGGA AGCATTACAA CACCAACCAC GAGGCTGATG 1321 ACTGGTGCGT CCCCAGCAGA GAACCCAAAG ACATGACGAC GTTCCGCAGT GCCTACCCAA 1381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1501 TATCTTGTGG AAAGGACGAC CGGTGAGACC GACCATTCAG AAAACGCGTT AAGTCgacaa 1561 tcaacctctg gattacaaaa tttgtgaaag att