Construct: ORF ccsbBroadEn_14737

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011391.2_s300c1, BRDN0000458536
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDK1 (5163)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5163 PDK1 pyruvate dehydrogenase kina... NM_002610.5 100% 100%
2 human 5163 PDK1 pyruvate dehydrogenase kina... NM_001278549.2 95.6% 95.3% 411_470del
3 human 5163 PDK1 pyruvate dehydrogenase kina... XM_011511345.3 82.5% 82.5% 0_1ins228
4 human 5163 PDK1 pyruvate dehydrogenase kina... XM_011511343.2 78.9% 78.7% 0_1ins228;183_242del
5 human 5163 PDK1 pyruvate dehydrogenase kina... XM_011511344.2 78.9% 78.7% 0_1ins228;183_242del
6 human 5163 PDK1 pyruvate dehydrogenase kina... XM_011511347.2 78.9% 78.7% 0_1ins228;183_242del
7 human 5163 PDK1 pyruvate dehydrogenase kina... XR_427094.1 78.1% (many diffs)
8 human 5163 PDK1 pyruvate dehydrogenase kina... XR_922943.2 77.5% 1_61del;472_531del;1430_1686del
9 human 5163 PDK1 pyruvate dehydrogenase kina... XR_001738772.2 75.6% 1_61del;472_531del;1430_1729del
10 human 5163 PDK1 pyruvate dehydrogenase kina... XR_001738775.2 74.4% 1_73del;1382_1758del
11 human 5163 PDK1 pyruvate dehydrogenase kina... XR_922945.1 72.4% 1_61del;472_531del;1430_1806del
12 human 5163 PDK1 pyruvate dehydrogenase kina... XR_001738773.1 71.9% 1_61del;472_531del;1430_1819del
13 human 5163 PDK1 pyruvate dehydrogenase kina... XR_922942.2 69.6% 1_61del;472_531del;1430_1877del
14 human 5163 PDK1 pyruvate dehydrogenase kina... XM_006712594.2 56.8% 55.7% (many diffs)
15 human 5163 PDK1 pyruvate dehydrogenase kina... XM_006712595.3 55.6% 54.8% (many diffs)
16 human 5163 PDK1 pyruvate dehydrogenase kina... NR_103731.1 54.5% (many diffs)
17 human 5163 PDK1 pyruvate dehydrogenase kina... NR_103729.2 44.3% 1_61del;1370_2952del
18 human 5163 PDK1 pyruvate dehydrogenase kina... XR_427093.3 43.4% 1_61del;472_531del;1430_3008del
19 human 5163 PDK1 pyruvate dehydrogenase kina... XR_001738776.1 40.9% (many diffs)
20 human 5163 PDK1 pyruvate dehydrogenase kina... XR_001738774.2 39.1% 1_73del;1382_3337del
21 human 5163 PDK1 pyruvate dehydrogenase kina... XR_922944.2 38.6% 1_61del;472_531del;1430_3385del
22 human 5163 PDK1 pyruvate dehydrogenase kina... XR_001738771.1 36.1% 1_61del;472_531del;1430_3619del
23 human 5163 PDK1 pyruvate dehydrogenase kina... XR_427095.3 28.9% 1_73del;841_842ins77;1305_4168del
24 mouse 228026 Pdk1 pyruvate dehydrogenase kina... NM_172665.5 85.6% 91.5% (many diffs)
25 mouse 228026 Pdk1 pyruvate dehydrogenase kina... XM_011239444.1 79% 84.3% (many diffs)
26 mouse 228026 Pdk1 pyruvate dehydrogenase kina... XM_017317590.1 72.4% 78.6% (many diffs)
27 mouse 228026 Pdk1 pyruvate dehydrogenase kina... XM_011239445.2 66.8% 72.5% (many diffs)
28 mouse 228026 Pdk1 pyruvate dehydrogenase kina... XM_011239446.2 66.8% 72.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1374
ORF length:
1308
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgag gctggcgcgg ctgcttcgcg gagccgcctt ggccggcccg ggcccggggc
121tgcgcgccgc cggcttcagc cgcagcttca gctcggactc gggctccagc ccggcgtccg
181agcgcggcgt tccgggccag gtggacttct acgcgcgctt ctcgccgtcc ccgctctcca
241tgaagcagtt cctggacttc ggatcagtga atgcttgtga aaagacctca tttatgtttc
301tgcggcaaga gttgcctgtc agactggcaa atataatgaa agaaataagt ctccttccag
361ataatcttct caggacacca tccgttcaat tggtacaaag ctggtatatc cagagtcttc
421aggagcttct tgattttaag gacaaaagtg ctgaggatgc taaagctatt tatgacttta
481cagatactgt gatacggatc agaaaccgac acaatgatgt cattcccaca atggcccagg
541gtgtgattga atacaaggag agctttgggg tggatcctgt caccagccag aatgttcagt
601actttttgga tcgattctac atgagtcgca tttcaattag aatgttactc aatcagcact
661ctttattgtt tggtggaaaa ggcaaaggaa gtccatctca tcgaaaacac attggaagca
721taaatccaaa ctgcaatgta cttgaagtta ttaaagatgg ctatgaaaat gctaggcgtc
781tgtgtgattt gtattatatt aactctcccg aactagaact tgaagaacta aatgcaaaat
841caccaggaca gccaatacaa gtggtttatg taccatccca tctctatcac atggtgtttg
901aacttttcaa gaatgcaatg agagccacta tggaacacca tgccaacaga ggtgtttacc
961cccctattca agttcatgtc acgctgggta atgaggattt gactgtgaag atgagtgacc
1021gaggaggtgg cgttcctttg aggaaaattg acagactttt caactacatg tattcaactg
1081caccaagacc tcgtgttgag acctcccgcg cagtgcctct ggctggtttt ggttatggat
1141tgcccatatc acgtctttac gcacaatact tccaaggaga cctgaagctg tattccctag
1201agggttacgg gacagatgca gttatctaca ttaaggctct gtcaacagac tcaatagaaa
1261gactcccagt gtataacaaa gctgcctgga agcattacaa caccaaccac gaggctgatg
1321actggtgcgt ccccagcaga gaacccaaag acatgacgac gttccgcagt gcctacccaa
1381ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac

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