Construct: ORF TRCN0000481554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001790.2_s317c1
- Derived from:
- ccsbBroadEn_04914
- DNA Barcode:
- CGAAGTCCTTGCTTCCGCCAACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RALYL (138046)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481554
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001100392.2 | 100% | 100% | |
| 2 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001100393.2 | 100% | 100% | |
| 3 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354305.1 | 100% | 100% | |
| 4 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354306.1 | 100% | 100% | |
| 5 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354308.1 | 100% | 100% | |
| 6 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354316.1 | 100% | 100% | |
| 7 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354320.1 | 100% | 100% | |
| 8 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354325.1 | 100% | 100% | |
| 9 | human | 138046 | RALYL | RALY RNA binding protein like | NM_173848.7 | 100% | 100% | |
| 10 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354314.1 | 99.6% | 99.6% | 683_684insAGA |
| 11 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001287243.2 | 96.2% | 95.8% | 331_332ins33 |
| 12 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354307.1 | 96.2% | 95.8% | 331_332ins33 |
| 13 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354309.1 | 96.2% | 95.8% | 331_332ins33 |
| 14 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354312.1 | 96.2% | 95.8% | 331_332ins33 |
| 15 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354315.1 | 96.2% | 95.8% | 331_332ins33 |
| 16 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354317.1 | 96.2% | 95.8% | 331_332ins33 |
| 17 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354318.1 | 96.2% | 95.8% | 331_332ins33 |
| 18 | human | 138046 | RALYL | RALY RNA binding protein like | XM_017013068.1 | 96.2% | 95.8% | 331_332ins33 |
| 19 | human | 138046 | RALYL | RALY RNA binding protein like | XM_017013071.1 | 96.2% | 95.8% | 331_332ins33 |
| 20 | human | 138046 | RALYL | RALY RNA binding protein like | XM_024447065.1 | 96.2% | 95.8% | 331_332ins33 |
| 21 | human | 138046 | RALYL | RALY RNA binding protein like | XM_024447066.1 | 96.2% | 95.8% | 331_332ins33 |
| 22 | human | 138046 | RALYL | RALY RNA binding protein like | XM_024447067.1 | 96.2% | 95.8% | 331_332ins33 |
| 23 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001100391.2 | 95.7% | 95.7% | 1_39del |
| 24 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354321.1 | 92.1% | 91.7% | 1_39del;370_371ins33 |
| 25 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354311.1 | 79.9% | 78.6% | (many diffs) |
| 26 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354313.1 | 79.9% | 78.6% | (many diffs) |
| 27 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354319.1 | 79.9% | 78.6% | (many diffs) |
| 28 | human | 138046 | RALYL | RALY RNA binding protein like | XM_011517462.3 | 77.4% | 75.6% | (many diffs) |
| 29 | human | 138046 | RALYL | RALY RNA binding protein like | XM_017013080.2 | 77.4% | 75.6% | (many diffs) |
| 30 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354323.1 | 76.5% | 75.3% | (many diffs) |
| 31 | human | 138046 | RALYL | RALY RNA binding protein like | XM_017013081.2 | 76.2% | 74.5% | (many diffs) |
| 32 | human | 138046 | RALYL | RALY RNA binding protein like | XM_017013082.1 | 76.2% | 74.5% | (many diffs) |
| 33 | human | 138046 | RALYL | RALY RNA binding protein like | XM_024447068.1 | 74.1% | 72.3% | (many diffs) |
| 34 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001287244.1 | 73% | 71.9% | (many diffs) |
| 35 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354322.1 | 72.9% | 71.3% | (many diffs) |
| 36 | human | 138046 | RALYL | RALY RNA binding protein like | NM_001354310.1 | 69.7% | 69.7% | 0_1ins264 |
| 37 | human | 138046 | RALYL | RALY RNA binding protein like | XM_017013084.2 | 69.3% | 67.8% | (many diffs) |
| 38 | human | 138046 | RALYL | RALY RNA binding protein like | XM_024447069.1 | 68.3% | 67.3% | (many diffs) |
| 39 | human | 138046 | RALYL | RALY RNA binding protein like | XM_024447070.1 | 65.9% | 65.6% | 0_1ins264;67_68ins33 |
| 40 | mouse | 76897 | Ralyl | RALY RNA binding protein-like | NM_001163328.1 | 88% | 94.8% | (many diffs) |
| 41 | mouse | 76897 | Ralyl | RALY RNA binding protein-like | NM_178631.4 | 88% | 94.8% | (many diffs) |
| 42 | mouse | 76897 | Ralyl | RALY RNA binding protein-like | XM_011248150.2 | 88% | 94.8% | (many diffs) |
| 43 | mouse | 76897 | Ralyl | RALY RNA binding protein-like | XM_017319801.1 | 88% | 94.8% | (many diffs) |
| 44 | mouse | 76897 | Ralyl | RALY RNA binding protein-like | XM_006530100.1 | 84% | 90.5% | (many diffs) |
| 45 | mouse | 76897 | Ralyl | RALY RNA binding protein-like | XM_006530101.1 | 80.7% | 86.6% | (many diffs) |
| 46 | mouse | 76897 | Ralyl | RALY RNA binding protein-like | XM_017319802.1 | 70.9% | 72.8% | (many diffs) |
| 47 | mouse | 76897 | Ralyl | RALY RNA binding protein-like | XM_017319803.1 | 70.3% | 76.4% | (many diffs) |
| 48 | mouse | 76897 | Ralyl | RALY RNA binding protein-like | XM_006530102.1 | 67.8% | 69.6% | (many diffs) |
| 49 | mouse | 76897 | Ralyl | RALY RNA binding protein-like | XM_006530103.1 | 67.1% | 72.9% | (many diffs) |
| 50 | mouse | 76897 | Ralyl | RALY RNA binding protein-like | NM_001163329.1 | 66% | 67.3% | (many diffs) |
| 51 | mouse | 76897 | Ralyl | RALY RNA binding protein-like | NM_001163330.1 | 48.3% | 48.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 939
- ORF length:
- 873
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tggcaaaaca cagaccagca acgtcaccaa taagaatgac cccaagtcca 121 tcaactcccg tgttttcatc ggcaatctaa atacggcaat tgtcaagaaa gttgacattg 181 aagccatttt ttcaaagtat ggaaaaatag ttggatgttc cgttcacaaa ggttatgcat 241 ttgtacagta catgagtgag cgacatgcaa gagctgcagt ggctggagaa aatgccagag 301 tcatcgccgg ccaaccactt gatatcaaca tggcaggaga gcccaaacca tacagaccaa 361 aacctggaaa caagaggccc ctttctgcac tttacagact tgaatcaaag gaacctttcc 421 tgtctgttgg cggttatgtc tttgactatg attactacag agatgatttc tacaatcggt 481 tatttgatta ccacgggcgt gtgcctccac cTCCCCGTGC AGTAATTCCG CTGAAGCGTC 541 CCAGAGTGGC AGTCACAACG ACTCGCAGGG GGAAAGGAGT CTTTTCCATG AAAGGTGGAT 601 CGAGATCTAC TGCCAGTGGG TCAACAGGTT CTAAATTGAA ATCAGATGAG TTACAGACCA 661 TCAAGAAAGA ATTAACCCAG ATCAAAACTA AAATTGACTC CTTGCTAGGG CGCCTGGAGA 721 AGATTGAGAA ACAGCAGAAG GCGGAGGCAG AAGCTCAGAA GAAGCAATTG GAAGAGAGTC 781 TAGTGCTGAT CCAAGAGGAA TGTGTGTCAG AGATTGCAGA TCACTCTACA GAGGAGCCTG 841 CTGAAGGAGG GCCAGATGCC GATGGAGAAG AGATGACAGA TGGGATAGAG GAGGACTTCG 901 ATGAAGATGG GGGTCATGAG CTGTTTCTAC AGATAAAGTG CCCAACTTTC TTGTACAAAG 961 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1021 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1081 ACGACGAAGT CCTTGCTTCC GCCAACTTAC GCGTTAAGTC gacaatcaac ctctggatta 1141 caaaatttgt gaaagatt