Transcript: Mouse NM_178631.4

Mus musculus RALY RNA binding protein-like (Ralyl), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Ralyl (76897)
Length:
2519
CDS:
406..1287

Additional Resources:

NCBI RefSeq record:
NM_178631.4
NBCI Gene record:
Ralyl (76897)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_178631.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000112044 CGGCAATTTGAATACAGCCAT pLKO.1 480 CDS 100% 2.640 3.696 N Ralyl n/a
2 TRCN0000112041 GCTTGAATCAAAGGAGCCATT pLKO.1 738 CDS 100% 4.050 3.240 N Ralyl n/a
3 TRCN0000112042 CGAGCTGTTTCTACAGATAAA pLKO.1 1263 CDS 100% 13.200 9.240 N Ralyl n/a
4 TRCN0000112043 CGATTACTACAGAGATGATTT pLKO.1 789 CDS 100% 13.200 9.240 N Ralyl n/a
5 TRCN0000112040 GCTATATTTCTGCCCTCTATA pLKO.1 1777 3UTR 100% 13.200 9.240 N Ralyl n/a
6 TRCN0000074367 GCTCAGAAGAAGCAATTGGAA pLKO.1 1099 CDS 100% 3.000 2.100 N RALYL n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_178631.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04914 pDONR223 100% 88% 94.8% None (many diffs) n/a
2 ccsbBroad304_04914 pLX_304 0% 88% 94.8% V5 (many diffs) n/a
3 TRCN0000481554 CGAAGTCCTTGCTTCCGCCAACTT pLX_317 49% 88% 94.8% V5 (many diffs) n/a
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