Construct: ORF TRCN0000481556

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010893.1_s317c1
Derived from:
ccsbBroadEn_07291
DNA Barcode:
CATGTAGGCATCATCTGTCAAGTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RNGTT (8732)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481556
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8732 RNGTT RNA guanylyltransferase and... NM_003800.5 99.8% 99.6% 634C>A;1781G>A
2 human 8732 RNGTT RNA guanylyltransferase and... XM_017011401.2 98.8% 98.6% 634C>A;1339_1356del;1799G>A
3 human 8732 RNGTT RNA guanylyltransferase and... NM_001286426.2 96% 95.8% 634C>A;1269_1270ins69;1712G>A
4 human 8732 RNGTT RNA guanylyltransferase and... XM_017011402.2 87.9% 80.5% (many diffs)
5 human 8732 RNGTT RNA guanylyltransferase and... NM_001286428.2 85.9% 85.7% (many diffs)
6 human 8732 RNGTT RNA guanylyltransferase and... XR_942617.3 39.7% (many diffs)
7 human 8732 RNGTT RNA guanylyltransferase and... XR_001743703.2 39.5% (many diffs)
8 mouse 24018 Rngtt RNA guanylyltransferase and... NM_011884.3 91% 94.9% (many diffs)
9 mouse 24018 Rngtt RNA guanylyltransferase and... NM_001305273.1 87.2% 87.3% (many diffs)
10 mouse 24018 Rngtt RNA guanylyltransferase and... XM_017320189.1 71.4% 71.5% (many diffs)
11 mouse 24018 Rngtt RNA guanylyltransferase and... XM_006537889.3 70.5% 69.5% (many diffs)
12 mouse 24018 Rngtt RNA guanylyltransferase and... XM_006537890.3 69.7% 71.2% (many diffs)
13 mouse 24018 Rngtt RNA guanylyltransferase and... XM_011250015.2 66.5% 67.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1857
ORF length:
1791
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tcacaacaag atcccgccgc ggtggctgaa ctgtccccgg cgcggccagc
121cggtggcagg aagattctta cctctgaaga caatgttagg accaagatat gatagtcaag
181ttgctgaaga aaatcggttc catcccagca tgctctcaaa ttacctaaag agcctaaagg
241ttaaaatggg cttgttggtg gacctgacaa atacttcaag gttctatgac cgaaatgaca
301tagaaaaaga aggaatcaaa tatataaaac ttcagtgtaa aggacatggt gagtgcccta
361ccactgagaa tactgagacc tttattcgtc tgtgtgagcg gtttaatgaa agaaatccac
421ctgaacttat aggtgttcat tgtactcatg gcttcaatcg cactggtttc ctcatatgtg
481cctttttggt ggagaaaatg gattggagta tcgaagcagc agttgctact tttgcccaag
541ccagaccacc aggaatctac aagggtgatt atttgaagga actttttcgt cggtatggtg
601acatagagga agcaccaccc ccacctctat tgccagattg gtgttttgag gatgatgaag
661acgaagatga ggatgaggat ggaaagaagg aatcagaaac cgggtcaagt gcttcttttg
721gcaaaaggag aaaagaacgg ttaaaactgg gcgctatttt cttggaaggt gttactgtta
781aaggtgtaac tcaagtaaca acacaaccaa agttaggaga ggtacagcag aagtgtcatc
841aattctgtgg ctgggaaggg tctggattcc ctggagcaca gcctgtttcc atggacaagc
901aaaatattaa acttttagac ctgaagccat acaaagtaag ctggaaagca gatggtactc
961ggtacatgat gttgattgat ggcacaaatg aagtttttat gattgataga gacaattcag
1021tatttcatgt ttcaaatctg gaatttccat ttcgtaaaga tcttcgtatg catttatcaa
1081atactctctt ggatggcgag atgattattg acagagtaaa tggacaggct gttcctagat
1141atttgatata tgacataatt aaattcaatt cacagcccgt tggagattgt gattttaatg
1201ttcgtctgca gtgtatagaa cgagaaatta taagtcctcg acacgaaaaa atgaagactg
1261ggctcattga caaaacacag gaaccattta gcgtcagaaa taagccgttt tttgacatct
1321gtacttcaag aaagctactt gaaggaaatt ttgccaaaga agtgagccat gaaatggatg
1381gacttatttt tcagcctact ggaaaataca aacctggtcg atgtgatgat attttgaaat
1441ggaagcctcc cagtctgaat tctgtggatt ttcgtctaaa aataacaaga atgggaggag
1501aagggttact tcctcagaat gttggcctcc tgtatgttgg aggttatgaa agaccctttg
1561cacaaatcaa ggtgacaaaa gagctgaaac agtatGACAA CAAAATTATA GAATGCAAAT
1621TTGAGAACAA CAGCTGGGTC TTCATGAGAC AGAGAACAGA CAAAAGTTTT CCTAATGCCT
1681ACAACACTGC CATGGCTGTG TGTAACAGCA TCTCAAACCC TGTCACCAAG GAGATGCTGT
1741TTGAGTTCAT CGACAGATGT ACTGCAGCTT CTCAAGGACA GAAGCGAAAA CATCATCTGG
1801ACCCTGACAC GGAGCTCATG CCACCACCAC CTCCCAAAAG ACCACACCCT TTAACCTACC
1861CAACTTGCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1921TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1981ATATATCTTG TGGAAAGGAC GACATGTAGG CATCATCTGT CAAGTAACGC GTTAAGTCga
2041caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)