Construct: ORF TRCN0000481556
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010893.1_s317c1
- Derived from:
- ccsbBroadEn_07291
- DNA Barcode:
- CATGTAGGCATCATCTGTCAAGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNGTT (8732)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481556
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 8732 | RNGTT | RNA guanylyltransferase and... | NM_003800.5 | 99.8% | 99.6% | 634C>A;1781G>A |
2 | human | 8732 | RNGTT | RNA guanylyltransferase and... | XM_017011401.2 | 98.8% | 98.6% | 634C>A;1339_1356del;1799G>A |
3 | human | 8732 | RNGTT | RNA guanylyltransferase and... | NM_001286426.2 | 96% | 95.8% | 634C>A;1269_1270ins69;1712G>A |
4 | human | 8732 | RNGTT | RNA guanylyltransferase and... | XM_017011402.2 | 87.9% | 80.5% | (many diffs) |
5 | human | 8732 | RNGTT | RNA guanylyltransferase and... | NM_001286428.2 | 85.9% | 85.7% | (many diffs) |
6 | human | 8732 | RNGTT | RNA guanylyltransferase and... | XR_942617.3 | 39.7% | (many diffs) | |
7 | human | 8732 | RNGTT | RNA guanylyltransferase and... | XR_001743703.2 | 39.5% | (many diffs) | |
8 | mouse | 24018 | Rngtt | RNA guanylyltransferase and... | NM_011884.3 | 91% | 94.9% | (many diffs) |
9 | mouse | 24018 | Rngtt | RNA guanylyltransferase and... | NM_001305273.1 | 87.2% | 87.3% | (many diffs) |
10 | mouse | 24018 | Rngtt | RNA guanylyltransferase and... | XM_017320189.1 | 71.4% | 71.5% | (many diffs) |
11 | mouse | 24018 | Rngtt | RNA guanylyltransferase and... | XM_006537889.3 | 70.5% | 69.5% | (many diffs) |
12 | mouse | 24018 | Rngtt | RNA guanylyltransferase and... | XM_006537890.3 | 69.7% | 71.2% | (many diffs) |
13 | mouse | 24018 | Rngtt | RNA guanylyltransferase and... | XM_011250015.2 | 66.5% | 67.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1857
- ORF length:
- 1791
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcacaacaag atcccgccgc ggtggctgaa ctgtccccgg cgcggccagc 121 cggtggcagg aagattctta cctctgaaga caatgttagg accaagatat gatagtcaag 181 ttgctgaaga aaatcggttc catcccagca tgctctcaaa ttacctaaag agcctaaagg 241 ttaaaatggg cttgttggtg gacctgacaa atacttcaag gttctatgac cgaaatgaca 301 tagaaaaaga aggaatcaaa tatataaaac ttcagtgtaa aggacatggt gagtgcccta 361 ccactgagaa tactgagacc tttattcgtc tgtgtgagcg gtttaatgaa agaaatccac 421 ctgaacttat aggtgttcat tgtactcatg gcttcaatcg cactggtttc ctcatatgtg 481 cctttttggt ggagaaaatg gattggagta tcgaagcagc agttgctact tttgcccaag 541 ccagaccacc aggaatctac aagggtgatt atttgaagga actttttcgt cggtatggtg 601 acatagagga agcaccaccc ccacctctat tgccagattg gtgttttgag gatgatgaag 661 acgaagatga ggatgaggat ggaaagaagg aatcagaaac cgggtcaagt gcttcttttg 721 gcaaaaggag aaaagaacgg ttaaaactgg gcgctatttt cttggaaggt gttactgtta 781 aaggtgtaac tcaagtaaca acacaaccaa agttaggaga ggtacagcag aagtgtcatc 841 aattctgtgg ctgggaaggg tctggattcc ctggagcaca gcctgtttcc atggacaagc 901 aaaatattaa acttttagac ctgaagccat acaaagtaag ctggaaagca gatggtactc 961 ggtacatgat gttgattgat ggcacaaatg aagtttttat gattgataga gacaattcag 1021 tatttcatgt ttcaaatctg gaatttccat ttcgtaaaga tcttcgtatg catttatcaa 1081 atactctctt ggatggcgag atgattattg acagagtaaa tggacaggct gttcctagat 1141 atttgatata tgacataatt aaattcaatt cacagcccgt tggagattgt gattttaatg 1201 ttcgtctgca gtgtatagaa cgagaaatta taagtcctcg acacgaaaaa atgaagactg 1261 ggctcattga caaaacacag gaaccattta gcgtcagaaa taagccgttt tttgacatct 1321 gtacttcaag aaagctactt gaaggaaatt ttgccaaaga agtgagccat gaaatggatg 1381 gacttatttt tcagcctact ggaaaataca aacctggtcg atgtgatgat attttgaaat 1441 ggaagcctcc cagtctgaat tctgtggatt ttcgtctaaa aataacaaga atgggaggag 1501 aagggttact tcctcagaat gttggcctcc tgtatgttgg aggttatgaa agaccctttg 1561 cacaaatcaa ggtgacaaaa gagctgaaac agtatGACAA CAAAATTATA GAATGCAAAT 1621 TTGAGAACAA CAGCTGGGTC TTCATGAGAC AGAGAACAGA CAAAAGTTTT CCTAATGCCT 1681 ACAACACTGC CATGGCTGTG TGTAACAGCA TCTCAAACCC TGTCACCAAG GAGATGCTGT 1741 TTGAGTTCAT CGACAGATGT ACTGCAGCTT CTCAAGGACA GAAGCGAAAA CATCATCTGG 1801 ACCCTGACAC GGAGCTCATG CCACCACCAC CTCCCAAAAG ACCACACCCT TTAACCTACC 1861 CAACTTGCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1921 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1981 ATATATCTTG TGGAAAGGAC GACATGTAGG CATCATCTGT CAAGTAACGC GTTAAGTCga 2041 caatcaacct ctggattaca aaatttgtga aagatt