Transcript: Mouse XM_017320189.1

PREDICTED: Mus musculus RNA guanylyltransferase and 5'-phosphatase (Rngtt), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Rngtt (24018)
Length:
2356
CDS:
377..1858

Additional Resources:

NCBI RefSeq record:
XM_017320189.1
NBCI Gene record:
Rngtt (24018)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017320189.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000220492 CCACTGAGAATACTGAGACAT pLKO.1 642 CDS 100% 4.950 3.960 N Rngtt n/a
2 TRCN0000220494 CGTCCCAAGATATTTGATCTA pLKO.1 1411 CDS 100% 4.950 3.960 N Rngtt n/a
3 TRCN0000439295 CAGTGTATTGAACGTGAAATT pLKO_005 1490 CDS 100% 13.200 9.240 N Rngtt n/a
4 TRCN0000272807 GGAATCTACAAGGGTGATTAT pLKO_005 833 CDS 100% 13.200 9.240 N RNGTT n/a
5 TRCN0000444560 TGGAATTTCCATTTCGTAAAG pLKO_005 1320 CDS 100% 10.800 7.560 N Rngtt n/a
6 TRCN0000220491 GCCATGAAATGGATGGACTTA pLKO.1 1647 CDS 100% 4.950 3.465 N Rngtt n/a
7 TRCN0000220493 CCAGGAATCTACAAGGGTGAT pLKO.1 830 CDS 100% 4.050 2.835 N Rngtt n/a
8 TRCN0000438977 ATGTTGATTGATGGCACAAAT pLKO_005 1250 CDS 100% 13.200 7.920 N Rngtt n/a
9 TRCN0000081181 CCAGACCACCAGGAATCTATA pLKO.1 822 CDS 100% 13.200 9.240 N LOC436408 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017320189.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07291 pDONR223 100% 71.4% 71.5% None (many diffs) n/a
2 ccsbBroad304_07291 pLX_304 0% 71.4% 71.5% V5 (many diffs) n/a
3 TRCN0000481556 CATGTAGGCATCATCTGTCAAGTA pLX_317 15% 71.4% 71.5% V5 (many diffs) n/a
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