Construct: ORF TRCN0000481558
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007610.2_s317c1
- Derived from:
- ccsbBroadEn_02603
- DNA Barcode:
- GCATATTCAGAGCCCGACATATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF2A1L (11036)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481558
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11036 | GTF2A1L | general transcription facto... | NM_006872.5 | 100% | 100% | |
| 2 | human | 11036 | GTF2A1L | general transcription facto... | NM_001193487.2 | 92.8% | 92.8% | 20_21ins102 |
| 3 | human | 286749 | STON1-GTF2A1L | STON1-GTF2A1L readthrough | NM_172311.3 | 40.2% | 40.1% | (many diffs) |
| 4 | human | 286749 | STON1-GTF2A1L | STON1-GTF2A1L readthrough | NM_001198593.1 | 37.3% | 36.9% | (many diffs) |
| 5 | human | 286749 | STON1-GTF2A1L | STON1-GTF2A1L readthrough | NM_001198594.1 | 36.2% | 36.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1500
- ORF length:
- 1434
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctgcctcaac ccggtgccta aactctacag atctgtaatt gaagatgtaa 121 ttgaaggagt tcggaatcta tttgctgaag aaggtataga ggaacaagtt ttaaaagact 181 tgaagcagct ctgggaaacc aaggttttgc agtctaaagc aacagaagac ttcttcagaa 241 atagcatcca atcacctctg tttactcttc agttgccgca cagcttgcac caaacattgc 301 aatcgtcaac agcatcatta gttattcctg ctggtagaac tcttccaagt tttaccacag 361 cagaactggg cacttcaaac tccagtgcaa actttacttt tcctggttat cccattcatg 421 taccagcagg tgtgacacta cagactgtat ctggtcacct ttataaagtc aatgtaccaa 481 ttatggtgac agagacttct ggaagagcag gtattcttca gcatccaatt cagcaagtat 541 ttcaacagct tggccagcct tcagtaatac aaactagtgt tccacaattg aatccatggt 601 ctcttcaagc aactactgaa aaatcacaga gaattgaaac cgtgctacag caacccgcaa 661 ttctaccttc tgggccagta gataggaaac acttagaaaa tgccaccagt gatatacttg 721 tatctcctgg aaatgagcat aaaatcgtgc ctgaagcttt gttgtgtcat caggaaagtt 781 ctcactatat cagtcttcca ggtgttgtat tttctccaca ggtctctcaa acaaattcta 841 atgtggagtc agtgctcagt ggttcagcta gcatggctca aaatctgcat gatgagtccc 901 tctccacaag ccctcatggg gctctccacc agcacgtgac tgatattcag cttcatattc 961 ttaaaaatag gatgtatgga tgtgattctg taaagcaacc aagaaatata gaggaaccca 1021 gcaacatacc tgtatcagag aaggattcta attctcaggt ggatttaagc attcgggtta 1081 ctgatgatga tattggtgaa ataattcaag tagatggaag cggtgataca tcttccaatg 1141 aagaaatagg aagtacaaga gatgcagatg agaatgaatt tctagggaat attgacgggg 1201 gagatctgaa ggtacctgaa gaagaagctg acagtatttc aaatgaggat tcagccacaa 1261 acagtagtga taatgaagac cctcaagtaa acattgtaga agaggaccct ttaaattctg 1321 gagatgatgt tagtgaacag gatgtgccag acctgtttga cacggataat gttattgtct 1381 gtcagtatga taagattcat cgaagcaaga acaaatggaa attctattTG AAAGATGGTG 1441 TTATGTGTTT TGGAGGGAGA GACTATGTAT TTGCAAAAGC CATTGGTGAT GCAGAGTGGT 1501 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1561 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1621 TTTATATATC TTGTGGAAAG GACGAGCATA TTCAGAGCCC GACATATGCA CGCGTTAAGT 1681 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt