Construct: ORF TRCN0000481578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003467.1_s317c1
- Derived from:
- ccsbBroadEn_11094
- DNA Barcode:
- CGCACCACACATAGACTCAAGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- REL (5966)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481578
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5966 | REL | REL proto-oncogene, NF-kB s... | NM_001291746.2 | 100% | 100% | |
| 2 | human | 5966 | REL | REL proto-oncogene, NF-kB s... | XM_017004627.2 | 96% | 96% | 921_922ins69 |
| 3 | human | 5966 | REL | REL proto-oncogene, NF-kB s... | NM_002908.4 | 94.8% | 94.8% | 922_1017del |
| 4 | human | 5966 | REL | REL proto-oncogene, NF-kB s... | XM_011533010.3 | 83.2% | 82.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1830
- ORF length:
- 1761
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctccggt gcgtataacc cgtatataga gataattgaa caacccaggc 121 agaggggaat gcgttttaga tacaaatgtg aagggcgatc agcaggcagc attccagggg 181 agcacagcac agacaacaac cgaacatacc cttctatcca gattatgaac tattatggaa 241 aaggaaaagt gagaattaca ttagtaacaa agaatgaccc atataaacct catcctcatg 301 atttagttgg aaaagactgc agagacggct actatgaagc agaatttgga caagaacgca 361 gacctttgtt tttccaaaat ttgggtattc gatgtgtgaa gaaaaaagaa gtaaaagaag 421 ctattattac aagaataaag gcaggaatca atccattcaa tgtccctgaa aaacagctga 481 atgatattga agattgtgac ctcaatgtgg tgagactgtg ttttcaagtt tttctccctg 541 atgaacatgg taatttgacg actgctcttc ctcctgttgt ctcgaaccca atttatgaca 601 accgtgctcc aaatactgca gaattaagga tttgtcgtgt aaacaagaat tgtggaagtg 661 tcagaggagg agatgaaata tttctacttt gtgacaaagt tcagaaagat gacatagaag 721 ttcgttttgt gttgaacgat tgggaagcaa aaggcatctt ttcacaagct gatgtacacc 781 gtcaagtagc cattgttttc aaaactccac catattgcaa agctatcaca gaacccgtaa 841 cagtaaaaat gcagttgcgg agaccttctg accaggaagt tagtgaatct atggatttta 901 gatatctgcc agatgaaaaa gatacttacg gcaataaagc aaagaaacaa aagacaactc 961 tgcttttcca gaaactgtgc caggatcacg ttaattttcc tgagagacca agacctggtc 1021 tcctcggttc aattggagaa ggaagatact tcaaaaaaga accaaacttg ttttctcatg 1081 atgcagttgt gagagaaatg cctacagggg tttcaagtca agcagaatcc tactatccct 1141 cacctgggcc catctcaagt ggattgtcac atcatgcctc aatggcacct ctgccttctt 1201 caagctggtc atcagtggcc caccccaccc cacgctcagg caatacaaac ccactgagta 1261 gtttttcaac aaggacactt ccttctaatt cgcaaggtat cccaccattc ctgagaatac 1321 ctgttgggaa tgatttaaat gcttctaatg cttgcattta caacaatgcc gatgacatag 1381 tcGGAATGGA AGCGTCATCC ATGCCATCAG CAGATTTATA TGGTATTTCT GATCCCAACA 1441 TGCTGTCTAA TTGTTCTGTG AATATGATGA CAACCAGCAG TGACAGCATG GGAGAGACTG 1501 ATAATCCAAG ACTTCTGAGC ATGAATCTTG AAAACCCCTC ATGTAATTCA GTGTTAGACC 1561 CAAGAGACTT GAGACAGCTC CATCAGATGT CCTCTTCCAG TATGTCAGCA GGCGCCAATT 1621 CCAATACTAC TGTTTTTGTT TCACAATCAG ATGCATTTGA GGGATCTGAC TTCAGTTGTG 1681 CAGATAACAG CATGATAAAT GAGTCGGGAC CATCAAACAG TACTAATCCA AACAGTCATG 1741 GTTTTGTTCA AGATAGTCAG TATTCAGGTA TTGGCAGTAT GCAAAATGAG CAATTGAGTG 1801 ACTCCTTTCC ATATGAATTT TTTCAAGTAT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1861 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1921 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACGCAC 1981 CACACATAGA CTCAAGCCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2041 tgaaagatt