Construct: ORF TRCN0000481594
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002170.1_s317c1
- Derived from:
- ccsbBroadEn_06814
- DNA Barcode:
- GAATTGCCAGGGTCGAAGCCGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTPN12 (5782)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481594
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5782 | PTPN12 | protein tyrosine phosphatas... | NM_002835.4 | 99.9% | 99.8% | 964G>A |
| 2 | human | 5782 | PTPN12 | protein tyrosine phosphatas... | XM_006716073.4 | 95.8% | 95.7% | 284_285ins96;868G>A |
| 3 | human | 5782 | PTPN12 | protein tyrosine phosphatas... | XM_005250518.2 | 85.2% | 83.2% | 0_1ins172;36_37ins173;619G>A |
| 4 | human | 5782 | PTPN12 | protein tyrosine phosphatas... | XM_017012474.2 | 85.2% | 83.2% | 0_1ins172;36_37ins173;619G>A |
| 5 | human | 5782 | PTPN12 | protein tyrosine phosphatas... | NM_001131008.2 | 84.7% | 84.6% | 0_1ins357;607G>A |
| 6 | human | 5782 | PTPN12 | protein tyrosine phosphatas... | NM_001131009.2 | 83.2% | 83.2% | 0_1ins390;574G>A |
| 7 | human | 5782 | PTPN12 | protein tyrosine phosphatas... | XR_001744844.2 | 66.7% | (many diffs) | |
| 8 | mouse | 19248 | Ptpn12 | protein tyrosine phosphatas... | NM_011203.2 | 84.5% | 83.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2406
- ORF length:
- 2340
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcaagtggag atcctgagga aattcatcca gagggtccag gccatgaaga 121 gtcctgacca caatggggag gacaacttcg cccgggactt catgcggtta agaagattgt 181 ctaccaaata tagaacagaa aagatatatc ccacagccac tggagaaaaa gaagaaaatg 241 ttaaaaagaa cagatacaag gacatactgc catttgatca cagccgagtt aaattgacat 301 taaagactcc ttcacaagat tcagactata tcaatgcaaa ttttataaag ggcgtctatg 361 ggccaaaagc atatgtagca actcaaggac ctttagcaaa tacagtaata gatttttgga 421 ggatgatatg ggagtataat gttgtgatca ttgtaatggc ctgccgagaa tttgagatgg 481 gaaggaaaaa atgtgagcgc tattggcctt tgtatggaga agaccccata acgtttgcac 541 catttaaaat ttcttgtgag gatgaacaag caagaacaga ctacttcatc aggacactct 601 tacttgaatt tcaaaatgaa tctcgtaggc tgtatcagtt tcattatgtg aactggccag 661 accatgatgt tccttcatca tttgattcta ttctggacat gataagctta atgaggaaat 721 atcaagaaca tgaagatgtt cctatttgta ttcattgcag tgcaggctgt ggaagaacag 781 gtgccatttg tgccatagat tatacgtgga atttactaaa agctgggaaa ataccagagg 841 aatttaatgt atttaattta atacaagaaa tgagaacaca aaggcattct gcagtacaaa 901 caaaggagca atatgaactt gttcatagag ctattgccca actgtttgaa aaacagctac 961 aactatatga aattcatgga gctcagaaaa ttgctgatgg agtgaatgaa attaacactg 1021 aaaacatgat cagctccata gagcctgaaa aacaagattc tcctcctcca aaaccaccaa 1081 ggacccgcag ttgccttgtt gaaggggatg ctaaagaaga aatactgcag ccaccggaac 1141 ctcatccagt gccacccatc ttgacacctt ctcccccttc agcttttcca acagtcacta 1201 ctgtgtggca ggacaatgat agataccatc caaagccagt gttgcatatg gtttcatcag 1261 aacaacattc agcagacctc aacagaaact atagtaaatc aacagaactt ccagggaaaa 1321 atgaatcaac aattgaacag atagataaaa aattggaacg aaatttaagt tttgagatta 1381 agaaggtccc tctccaagag ggaccaaaaa gttttgatgg gaacacactt ttgaataggg 1441 gacatgcaat taaaattaaa tctgcttcac cttgtatagc tgataaaatc tctaagccac 1501 aggaattaag ttcagatcta aatgtcggtg atacttccca gaattcttgt gtggactgca 1561 gtgtaacaca atcaaacaaa gtttcagtta ctccaccaga agaatcccag aattcagaca 1621 cacctccaag gccagaccgc ttgcctcttg atgagaaagg acatgtaacg tggtcatttc 1681 atggacctga aaatgccata cccatacctg atttatctga aggcaattcc tcagatatca 1741 actatcaaac taggaaaact gtgagtttaa caccaagtcc tacaacacaa gttgaaacac 1801 ctgatcttgt ggatcatgat aacacttcac cactcttcag aacacccctc agttttacta 1861 atccacttca ctctgatgac tcagactcag atgaaagaaa ctctgatggt gctgtgaccc 1921 agaataaaac taatatttca aCAGCAAGTG CCACAGTTTC TGCTGCCACT AGTACTGAAA 1981 GCATTTCTAC TAGGAAAGTA TTGCCAATGT CCATTGCTAG ACATAATATA GCAGGAACAA 2041 CACATTCAGG TGCTGAAAAA GATGTTGATG TTAGTGAAGA TTCACCTCCT CCCCTACCTG 2101 AAAGAACTCC TGAATCGTTT GTGTTAGCAA GTGAACATAA TACACCTGTA AGATCGGAAT 2161 GGAGTGAACT TCAAAGTCAG GAACGATCTG AACAAAAAAA GTCTGAAGGC TTGATAACCT 2221 CTGAAAATGA GAAATGTGAT CATCCAGCGG GAGGTATTCA CTATGAAATG TGCATAGAAT 2281 GTCCACCTAC TTTCAGTGAC AAGAGAGAAC AAATATCAGA AAATCCAACA GAAGCCACAG 2341 ATATTGGTTT TGGTAATCGA TGTGGAAAAC CCAAAGGACC AAGAGATCCA CCTTCAGAAT 2401 GGACATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2461 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2521 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGAATTGCCA GGGTCGAAGC CGTTAACGCG 2581 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt