Construct: ORF TRCN0000481611
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008922.1_s317c1
- Derived from:
- ccsbBroadEn_11509
- DNA Barcode:
- ACGCTCACTTGGTATTACATGAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA6C (10500)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481611
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10500 | SEMA6C | semaphorin 6C | NM_030913.5 | 46.7% | 44% | (many diffs) |
| 2 | human | 10500 | SEMA6C | semaphorin 6C | XM_017000079.1 | 46.7% | 44% | (many diffs) |
| 3 | human | 10500 | SEMA6C | semaphorin 6C | NM_001178061.2 | 45.2% | 42.6% | (many diffs) |
| 4 | human | 10500 | SEMA6C | semaphorin 6C | XM_005244835.3 | 45.2% | 42.6% | (many diffs) |
| 5 | human | 10500 | SEMA6C | semaphorin 6C | XM_017000075.2 | 45.2% | 42.6% | (many diffs) |
| 6 | human | 10500 | SEMA6C | semaphorin 6C | XM_017000076.2 | 45.2% | 42.6% | (many diffs) |
| 7 | human | 10500 | SEMA6C | semaphorin 6C | XM_017000077.1 | 45.2% | 42.6% | (many diffs) |
| 8 | human | 10500 | SEMA6C | semaphorin 6C | NM_001178062.2 | 41.1% | 38.4% | (many diffs) |
| 9 | human | 10500 | SEMA6C | semaphorin 6C | XM_017000081.1 | 41.1% | 38.4% | (many diffs) |
| 10 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | NM_011351.2 | 44.2% | 40.6% | (many diffs) |
| 11 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | XM_006501205.1 | 44.2% | 40.6% | (many diffs) |
| 12 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | NM_001272024.1 | 42.8% | 39.3% | (many diffs) |
| 13 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | XM_006501192.3 | 42.8% | 39.3% | (many diffs) |
| 14 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | XM_006501193.3 | 42.8% | 39.3% | (many diffs) |
| 15 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | XM_006501194.3 | 42.8% | 39.3% | (many diffs) |
| 16 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | XM_006501195.3 | 42.8% | 39.3% | (many diffs) |
| 17 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | XM_006501196.3 | 42.8% | 39.3% | (many diffs) |
| 18 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | XM_006501197.3 | 42.8% | 39.3% | (many diffs) |
| 19 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | XM_006501198.3 | 42.8% | 39.3% | (many diffs) |
| 20 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | XM_006501201.3 | 42.8% | 39.3% | (many diffs) |
| 21 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | XM_006501202.3 | 42.8% | 39.3% | (many diffs) |
| 22 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | XM_006501204.3 | 42.8% | 39.3% | (many diffs) |
| 23 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | NR_073551.1 | 30.9% | (many diffs) | |
| 24 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | NR_073549.1 | 30.7% | (many diffs) | |
| 25 | mouse | 20360 | Sema6c | sema domain, transmembrane ... | NR_073550.1 | 30.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1491
- ORF length:
- 1422
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccccgtgcc ccccacttca tgcccttgct gctactgctg ctgctgctct 121 cacttcccca tactcaggcc gcctttcccc aggaccccct ccctctgttg atctctgacc 181 ttcaaggtac ttccccatta tcctggtttc ggggcctgga ggatgatgct gtggctgcag 241 aacttgggct ggactttcag agattcctga ccttgaaccg gaccttgcta gtggctgccc 301 gggatcacgt tttctccttc gatcttcaag ccgaagaaga aggggagggg ctggtgccca 361 acaagtatct aacatggaga agccaagatg tggagaactg tgctgtacgg ggaaagctga 421 cggatgagtg ctacaactat attcgtgttc ttgttccctg ggactcccag acgctccttg 481 cctgtggaac gaactcattc agccctgtgt gccgcagcta tgggataact tcgctgcagc 541 aggagggtga ggaactgagt gggcaggctc gatgcccctt tgatgccacc cagtccaacg 601 tggccatctt tgcagagggc agcctgtact cagccacagc tgcggatttc caggccagtg 661 atgctgtagt ttacagaagc cttgggcccc agcccccact ccgctccgcc aagtatgact 721 ccaagtggct ccgagagcca cactttgtcc aggccttgga gcatggagac catgtctact 781 tcttcttccg cgaggtctct gtggaggatg ctcggctggg gagggtgcag ttctcccgcg 841 tagcccgagt atgtaaacgt gacatgggcg gctcgcctcg ggccttggac cgccactgga 901 catccttcct gaagcttcgg ctcaactgct ctgtccctgg ggactctact ttctattttg 961 atgttttaca ggccttgact gggcctgtga acctgcatgg ccgctctgct ctctttgggg 1021 tcttcaccac ccagaccaat agcatccctg gctctgccgt ctgcgcctcc tacctggatg 1081 agattgagcg tgggtttgag gGCAAGTTCA AGGAGCAGAG GAGTCTGGAT GGGGCCTGGA 1141 CTCCTGTGTC TGAGGACAGA GTTCCCTCAC CCAGGCCAGG ATCCTGTGCA GGAGTAGGGG 1201 GAGCTGCCTT GTTCTCCTCT TCCCGAGACC TCCCCTCGTC GGGCCCTCCT CCCGCCAGGC 1261 CGTCCCGAAC GGCGGCCGTT TCAACTTTTA AAGGGAGCGG CCCACGGCCT CCAGCGTGGG 1321 GAGCGCCCGA GTCCTCTCGG CCACGAGCTG GACGCTCTTC AGGACGTTTC ACCGCCCCCT 1381 CGCCCCGCAC CTCCAGCCTT CCCGACTCGC AGAGTCTCCC GAGGCCCCTT TTCGCCTCGG 1441 GTTTATTTAT TGACTGTCTT TCCCCCTGTC CTCGACAGAA GAGTGGGAGG TTGCCAACTT 1501 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1561 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1621 CTTGTGGAAA GGACGAACGC TCACTTGGTA TTACATGAGA ACGCGTTAAG TCgacaatca 1681 acctctggat tacaaaattt gtgaaagatt