Construct: ORF TRCN0000481622
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004164.1_s317c1
- Derived from:
- ccsbBroadEn_00382
- DNA Barcode:
- AGACTGACAGTCCTTCTTCCCCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSNK2A2 (1459)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481622
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1459 | CSNK2A2 | casein kinase 2 alpha 2 | NM_001896.4 | 100% | 100% | |
| 2 | human | 1459 | CSNK2A2 | casein kinase 2 alpha 2 | XM_005255800.4 | 85.4% | 85.4% | 213_214ins153 |
| 3 | human | 1459 | CSNK2A2 | casein kinase 2 alpha 2 | XM_017022945.2 | 65.1% | 62.1% | (many diffs) |
| 4 | human | 1459 | CSNK2A2 | casein kinase 2 alpha 2 | XM_005255801.3 | 60.8% | 60.8% | 0_1ins411 |
| 5 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | NM_009974.3 | 94.8% | 98.8% | (many diffs) |
| 6 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | XM_017312556.1 | 90% | 94% | (many diffs) |
| 7 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | XM_006530641.3 | 80.3% | 84.2% | (many diffs) |
| 8 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | XM_006530642.1 | 56.9% | 59.7% | (many diffs) |
| 9 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | XM_006530643.3 | 56.9% | 59.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cggcccggcc gcgggcagca gggcccgggt ctacgccgag gtgaacagtc 121 tgaggagccg cgagtactgg gactacgagg ctcacgtccc gagctggggt aatcaagatg 181 attaccaact ggttcgaaaa cttggtcggg gaaaatatag tgaagtattt gaggccatta 241 atatcaccaa caatgagaga gtggttgtaa aaatcctgaa gccagtgaag aaaaagaaga 301 taaaacgaga ggttaagatt ctggagaacc ttcgtggtgg aacaaatatc attaagctga 361 ttgacactgt aaaggacccc gtgtcaaaga caccagcttt ggtatttgaa tatatcaata 421 atacagattt taagcaactc taccagatcc tgacagactt tgatatccgg ttttatatgt 481 atgaactact taaagctctg gattactgcc acagcaaggg aatcatgcac agggatgtga 541 aacctcacaa tgtcatgata gatcaccaac agaaaaagct gcgactgata gattggggtc 601 tggcagaatt ctatcatcct gcTCAGGAGT ACAATGTTCG TGTAGCCTCA AGGTACTTCA 661 AGGGACCAGA GCTCCTCGTG GACTATCAGA TGTATGATTA TAGCTTGGAC ATGTGGAGTT 721 TGGGCTGTAT GTTAGCAAGC ATGATCTTTC GAAGGGAACC ATTCTTCCAT GGACAGGACA 781 ACTATGACCA GCTTGTTCGC ATTGCCAAGG TTCTGGGTAC AGAAGAACTG TATGGGTATC 841 TGAAGAAGTA TCACATAGAC CTAGATCCAC ACTTCAACGA TATCCTGGGA CAACATTCAC 901 GGAAACGCTG GGAAAACTTT ATCCATAGTG AGAACAGACA CCTTGTCAGC CCTGAGGCCC 961 TAGATCTTCT GGACAAACTT CTGCGATACG ACCATCAACA GAGACTGACT GCCAAAGAGG 1021 CCATGGAGCA CCCATACTTC TACCCTGTGG TGAAGGAGCA GTCCCAGCCT TGTGCAGACA 1081 ATGCTGTGCT TTCCAGTGGT CTCACGGCAG CACGATGCCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 AAGACTGACA GTCCTTCTTC CCCTGACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt