Transcript: Human XM_017022945.2

PREDICTED: Homo sapiens casein kinase 2 alpha 2 (CSNK2A2), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CSNK2A2 (1459)
Length:
2010
CDS:
818..1546

Additional Resources:

NCBI RefSeq record:
XM_017022945.2
NBCI Gene record:
CSNK2A2 (1459)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001144724 TGTGCATGATTCCCTTGCTG pXPR_003 TGG 125 17% 2 0.6989 CSNK2A2 CSNK2A2 75586
2 BRDN0001148502 AGTTTACCTGATAGTCCACG pXPR_003 AGG 290 40% 3 0.2447 CSNK2A2 CSNK2A2 75588
3 BRDN0001147190 TAGCAAGCATGATCTTTCGA pXPR_003 AGG 360 49% 4 -0.4310 CSNK2A2 CSNK2A2 75587
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017022945.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000382114 TCAAACGTCTTAACGCGTATA pLKO_005 1611 3UTR 100% 10.800 15.120 N CSNK2A2 n/a
2 TRCN0000000614 CTGGGACAACATTCACGGAAA pLKO.1 1313 CDS 100% 4.050 5.670 N CSNK2A2 n/a
3 TRCN0000318620 CTGGGACAACATTCACGGAAA pLKO_005 1313 CDS 100% 4.050 5.670 N CSNK2A2 n/a
4 TRCN0000025666 GTATGATTATAGCTTGGACAT pLKO.1 1120 CDS 100% 4.050 3.240 N Csnk2a2 n/a
5 TRCN0000000611 GCGGCAGTTACATATTATTAT pLKO.1 1949 3UTR 100% 15.000 10.500 N CSNK2A2 n/a
6 TRCN0000380736 ATCAAACCTCACTTCCGAATG pLKO_005 1721 3UTR 100% 6.000 4.200 N CSNK2A2 n/a
7 TRCN0000379457 ATGCAGAATGTTGTTGGTTAC pLKO_005 1814 3UTR 100% 6.000 4.200 N CSNK2A2 n/a
8 TRCN0000194896 CCTCACAATGTCATGATAGAT pLKO.1 971 CDS 100% 5.625 3.938 N CSNK2A2 n/a
9 TRCN0000318690 CCTCACAATGTCATGATAGAT pLKO_005 971 CDS 100% 5.625 3.938 N CSNK2A2 n/a
10 TRCN0000000615 AGACCTAGATCCACACTTCAA pLKO.1 1285 CDS 100% 4.950 3.465 N CSNK2A2 n/a
11 TRCN0000000612 CCTCGTGGACTATCAGATGTA pLKO.1 1102 CDS 100% 4.950 3.465 N CSNK2A2 n/a
12 TRCN0000318619 CCTCGTGGACTATCAGATGTA pLKO_005 1102 CDS 100% 4.950 3.465 N CSNK2A2 n/a
13 TRCN0000197008 GCAGAATGTTGTTGGTTACTG pLKO.1 1816 3UTR 100% 4.950 3.465 N CSNK2A2 n/a
14 TRCN0000025668 CAGGACAACTATGACCAGCTT pLKO.1 1202 CDS 100% 2.640 1.848 N Csnk2a2 n/a
15 TRCN0000321792 CAGGACAACTATGACCAGCTT pLKO_005 1202 CDS 100% 2.640 1.848 N Csnk2a2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017022945.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00382 pDONR223 100% 65.1% 62.1% None (many diffs) n/a
2 ccsbBroad304_00382 pLX_304 0% 65.1% 62.1% V5 (many diffs) n/a
3 TRCN0000481622 AGACTGACAGTCCTTCTTCCCCTG pLX_317 47.1% 65.1% 62.1% V5 (many diffs) n/a
4 TRCN0000489293 CACACGAACCTGTACAGAGAAATG pLX_317 35% 65.1% 61.9% V5 (many diffs) n/a
5 TRCN0000492298 ACCTTTATGCCGATCAATACAAAT pLX_317 41.1% 65% 61.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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