Construct: ORF TRCN0000481643
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015555.1_s317c1
- Derived from:
- ccsbBroadEn_13813
- DNA Barcode:
- GATAACGTGACCGCTAGGGGTCCA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARAF (369)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481643
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 369 | ARAF | A-Raf proto-oncogene, serin... | NM_001256196.1 | 99.9% | 99.5% | 1817delG |
| 2 | human | 369 | ARAF | A-Raf proto-oncogene, serin... | NM_001654.5 | 99.4% | 99% | 458_459insGCCCTCAAG;1808delG |
| 3 | human | 369 | ARAF | A-Raf proto-oncogene, serin... | NM_001256197.1 | 30.5% | 30.3% | 458_459insGCCCTCAAG;558_558delGins1260 |
| 4 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | NM_009703.2 | 89.4% | 94.2% | (many diffs) |
| 5 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | XM_006527556.3 | 89.4% | 94.2% | (many diffs) |
| 6 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | XM_006527557.3 | 89.4% | 94.2% | (many diffs) |
| 7 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | XM_011247429.2 | 84.3% | 88.8% | (many diffs) |
| 8 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | XM_011247430.2 | 66.8% | 68.8% | (many diffs) |
| 9 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | NM_001159645.1 | 27.6% | 28.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1893
- ORF length:
- 1827
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gccaccacgg ggcccccctg ccaatggggc cgagccatcc cgggcagtgg 121 gcaccgtcaa agtatacctg cccaacaagc aacgcacggt ggtgactgtc cgggatggca 181 tgagtgtcta cgactctcta gacaaggccc tgaaggtgcg gggtctaaat caggactgct 241 gtgtggtcta ccgactcatc aagggacgaa agacggtcac tgcctgggac acagccattg 301 ctcccctgga tggcgaggag ctcattgtcg aggtccttga agatgtcccg ctgaccatgc 361 acaattttgt acggaagacc ttcttcagcc tggcgttctg tgacttctgc cttaagtttc 421 tgttccatgg cttccgttgc caaacctgtg gctacaagtt ccaccagcat tgttcctcca 481 aggtccccac agtctgtgtt gacatgagta ccaaccgcca acagccctca aggttctacc 541 acagtgtcca ggatttgtcc ggaggctcca gacagcatga ggctccctcg aaccgccccc 601 tgaatgagtt gctaaccccc cagggtccca gcccccgcac ccagcactgt gacccggagc 661 acttcccctt ccctgcccca gccaatgccc ccctacagcg catccgctcc acgtccactc 721 ccaacgtcca tatggtcagc accacggccc ccatggactc caacctcatc cagctcactg 781 gccagagttt cagcactgat gctgccggta gtagaggagg tagtgatgga accccccggg 841 ggagccccag cccagccagc gtgtcctcgg ggaggaagtc cccacattcc aagtcaccag 901 cagagcagcg cgagcggaag tccttggccg atgacaagaa gaaagtgaag aacctggggt 961 accgggactc aggctattac tgggaggtac cacccagtga ggtgcagctg ctgaagagga 1021 tcgggacggg ctcgtttggc accgtgtttc gagggcggtg gcatggcgat gtggccgtga 1081 aggtgctcaa ggtgtcccag cccacagctg agcaggccca ggctttcaag aatgagatgc 1141 aggtgctcag gaagacgcga catgtcaaca tcttgctgtt tatgggcttc atgacccggc 1201 cgggatttgc catcatcaca cagtggtgtg agggctccag cctctaccat cacctgcatg 1261 tggccgacac acgcttcgac atggtccagc tcatcgacgt ggcccggcag actgcccagg 1321 gcatggacta cctccatgcc aagaacatca tccaccgaga tctcaagtct aacaacatct 1381 tcctacatga ggggctcacg gtgaagatcg gtgactttgg cttggccaca gtgaagactc 1441 gatggagcgg ggcccagccc ttggagcagc cctcaggatc tgtgctgtgg atggcagctg 1501 aggtgatccg tatgcaggac ccgaacccct acagcttcca gtcagacgtc tatgcctacg 1561 gggttgtgct cTACGAGCTT ATGACTGGCT CACTGCCTTA CAGCCACATT GGCTGCCGTG 1621 ACCAGATTAT CTTTATGGTG GGCCGTGGCT ATCTGTCCCC GGACCTCAGC AAAATCTCCA 1681 GCAACTGCCC CAAGGCCATG CGGCGCCTGC TGTCTGACTG CCTCAAGTTC CAGCGGGAGG 1741 AGCGGCCCCT CTTCCCCCAG ATCCTGGCCA CAATTGAGCT GCTGCAACGG TCACTCCCCA 1801 AGATTGAGCG GAGTGCCTCG GAACCCTCCT TGCACCGCAC CCAGGCCGAT GAGTTGCCTG 1861 CCTGCCTACT CAGCGCAGCC CCCTTGTGCC TTACCCAACT TTCTTGTACA AAGTGGTTGA 1921 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 1981 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAT 2041 AACGTGACCG CTAGGGGTCC AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 2101 tgtgaaagat t