Construct: ORF TRCN0000481654
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011822.1_s317c1
- Derived from:
- ccsbBroadEn_09833
- DNA Barcode:
- GCAGAATGGCCTTGGGGTCATGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SENP5 (205564)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481654
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 205564 | SENP5 | SUMO specific peptidase 5 | NM_152699.5 | 99.9% | 99.8% | 224C>A |
| 2 | human | 205564 | SENP5 | SUMO specific peptidase 5 | XM_011512544.3 | 99.9% | 99.8% | 224C>A |
| 3 | human | 205564 | SENP5 | SUMO specific peptidase 5 | XM_005269310.3 | 96.2% | 96.1% | 224C>A;2020_2021ins84 |
| 4 | human | 205564 | SENP5 | SUMO specific peptidase 5 | NM_001308045.1 | 93.8% | 93.7% | 224C>A;1882_1883ins138 |
| 5 | mouse | 320213 | Senp5 | SUMO/sentrin specific pepti... | NM_177103.4 | 86.7% | 81.7% | (many diffs) |
| 6 | mouse | 320213 | Senp5 | SUMO/sentrin specific pepti... | XM_006522238.3 | 86.7% | 81.7% | (many diffs) |
| 7 | mouse | 320213 | Senp5 | SUMO/sentrin specific pepti... | XM_006522239.3 | 86.7% | 81.7% | (many diffs) |
| 8 | mouse | 320213 | Senp5 | SUMO/sentrin specific pepti... | XM_011245933.2 | 86.7% | 81.7% | (many diffs) |
| 9 | mouse | 320213 | Senp5 | SUMO/sentrin specific pepti... | XM_006522240.3 | 83.6% | 78.3% | (many diffs) |
| 10 | mouse | 320213 | Senp5 | SUMO/sentrin specific pepti... | XM_006522242.3 | 81.1% | 75.7% | (many diffs) |
| 11 | mouse | 320213 | Senp5 | SUMO/sentrin specific pepti... | XM_017317035.1 | 76.7% | 71.1% | (many diffs) |
| 12 | mouse | 320213 | Senp5 | SUMO/sentrin specific pepti... | XM_006522243.3 | 71.2% | 65% | (many diffs) |
| 13 | mouse | 320213 | Senp5 | SUMO/sentrin specific pepti... | XR_875823.2 | 67.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2331
- ORF length:
- 2265
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa aaaacagagg aaaattctat ggaggaaagg aatccactta gccttttctg 121 agaaatggaa tactgggttt ggaggcttta agaagtttta ttttcaccaa cacttgtgca 181 ttctgaaagc taagctggga aggccagtta cttggaatag acagttgaga catttccagg 241 gtagaaagaa agctcttcaa atccagaaaa cgtggatcaa ggatgaacac ctttgtgcta 301 agaccaagtt caatgtggct actcaaaatg ttagtacttt gtcctctaaa gtgaaaagaa 361 aggacgctaa acacttcatt tcctcctcaa agactctcct gagactccaa gcagagaagc 421 tgttgtcatc agcaaagaat tctgaccatg aatactgcag agagaaaaat ctcttgaagg 481 cagttactga ctttccatca aatagtgctt taggtcaggc caatggtcac agacctagga 541 cagacccaca accttctgac tttcccatga agttcaatgg ggagagccaa agtccaggtg 601 agagtggcac gattgtggtc accttgaaca accataagag aaagggcttt tgttacggct 661 gctgccaagg gccggagcac cacaggaatg ggggaccctt gattccaaaa aagttccaac 721 ttaaccaaca tagaaggata aaattatctc ctcttatgat gtatgagaaa ttatccatga 781 ttagatttcg gtacaggatt ctcagatccc agcacttcag aaccaaaagc aaggtttgca 841 agctaagaaa agcccagcga agctgggtac agaaagtcac tggggaccat caagagaccc 901 gtagggagaa cggtgagggt ggcagttgca gcccatttcc ttccccagaa cctaaagacc 961 cttcttgtcg gcatcagccg tactttccag atatggacag cagtgctgtg gtgaagggga 1021 cgaactctca tgtgcctgat tgccacacta aaggaagctc tttcttgggc aaggagctta 1081 gtttagacga agcattccct gaccaacaga atggcagtgc cacaaacgcc tgggaccagt 1141 catcctgttc ttctcctaag tgggagtgta cagagctgat tcatgacatc cccttaccag 1201 aacatcgttc taataccatg ttcatttcag aaactgaaag agaaattatg actctgggtc 1261 aggaaaatca gacaagttct gtcagtgatg acagagtaaa actgtcagtg tctggagcag 1321 atacatctgt gagtagcgta gatgggcctg tgtcccaaaa ggctgttcaa aatgagaact 1381 cataccagat ggaggaggat ggatctctca agcagagcat tcttagttct gagttgctgg 1441 accaccctta ctgtaaaagt ccactggagg ctcccttggt gtgcagtgga ctcaaactag 1501 aaaatcaagt aggaggtgga aagaacagtc agaaagcctc tccagtggat gatgaacagc 1561 tgtcagtctg tctttctgga ttcctagatg aggttatgaa gaagtatggc agtttggttc 1621 cactcagtga aaaagaagtc cttggaagat taaaagatgt ctttaatgaa gacttttcta 1681 atagaaaacc atttatcaat agggaaataa caaactatcg ggccagacat caaaaatgta 1741 acttccgtat cttctataat aaacacatgc tggatatgga cgacctggcg actctggatg 1801 gTCAGAACTG GCTGAATGAC CAGGTCATTA ATATGTATGG TGAGCTGATA ATGGATGCAG 1861 TCCCAGACAA AGTTCACTTC TTCAACAGCT TTTTTCATAG ACAGCTGGTA ACCAAAGGAT 1921 ATAATGGAGT AAAAAGATGG ACTAAAAAGG TGGATTTGTT TAAAAAGAGT CTTCTGTTGA 1981 TTCCTATTCA CCTGGAAGTC CACTGGTCTC TCATTACTGT GACACTCTCT AATCGAATTA 2041 TTTCATTTTA TGATTCCCAA GGCATTCATT TTAAGTTTTG TGTAGAGAAT ATAAGAAAGT 2101 ATTTGCTGAC TGAAGCCAGA GAAAAAAATA GACCTGAATT TCTTCAGGGT TGGCAGACTG 2161 CTGTTACGAA GTGTATTCCA CAACAGAAAA ACGACAGTGA CTGTGGAGTC TTTGTGCTCC 2221 AGTACTGCAA GTGCCTCGCC TTAGAGCAGC CTTTCCAGTT TTCACAAGAA GACATGCCCC 2281 GAGTGCGGAA GAGGATTTAC AAGGAGCTAT GTGAGTGCCG GCTCATGGAC TGCCCAACTT 2341 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2401 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2461 CTTGTGGAAA GGACGAGCAG AATGGCCTTG GGGTCATGGG ACGCGTTAAG TCgacaatca 2521 acctctggat tacaaaattt gtgaaagatt