Construct: ORF TRCN0000487688
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021969.1_s317c1
- DNA Barcode:
- TTTGAATATCGGGTTTTTTCCAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TCF7 (6932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487688
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6932 | TCF7 | transcription factor 7 | NM_003202.5 | 100% | 100% | |
| 2 | human | 6932 | TCF7 | transcription factor 7 | XM_006714684.2 | 94.3% | 93.2% | (many diffs) |
| 3 | human | 6932 | TCF7 | transcription factor 7 | NM_001346425.2 | 92.5% | 92.5% | 635_727del |
| 4 | human | 6932 | TCF7 | transcription factor 7 | XM_011543606.1 | 87.5% | 85.3% | (many diffs) |
| 5 | human | 6932 | TCF7 | transcription factor 7 | XM_006714682.2 | 87.3% | 86.2% | (many diffs) |
| 6 | human | 6932 | TCF7 | transcription factor 7 | XM_006714679.3 | 86.9% | 84.8% | (many diffs) |
| 7 | human | 6932 | TCF7 | transcription factor 7 | XM_011543604.2 | 86.9% | 85.8% | (many diffs) |
| 8 | human | 6932 | TCF7 | transcription factor 7 | XM_006714678.3 | 81.1% | 79.1% | (many diffs) |
| 9 | human | 6932 | TCF7 | transcription factor 7 | NM_201632.5 | 70% | 70% | 0_1ins345 |
| 10 | human | 6932 | TCF7 | transcription factor 7 | NM_213648.5 | 70% | 70% | 0_1ins345 |
| 11 | human | 6932 | TCF7 | transcription factor 7 | NM_001134851.4 | 66.4% | 62.4% | (many diffs) |
| 12 | human | 6932 | TCF7 | transcription factor 7 | NM_201634.5 | 65% | 63.4% | (many diffs) |
| 13 | human | 6932 | TCF7 | transcription factor 7 | XR_001742231.1 | 64.8% | 1_204del;645_754del;1265_1266ins201 | |
| 14 | human | 6932 | TCF7 | transcription factor 7 | XR_948294.2 | 63.3% | (many diffs) | |
| 15 | human | 6932 | TCF7 | transcription factor 7 | NM_001366502.1 | 62.9% | 61.9% | (many diffs) |
| 16 | human | 6932 | TCF7 | transcription factor 7 | XR_001742232.1 | 61% | (many diffs) | |
| 17 | human | 6932 | TCF7 | transcription factor 7 | XM_006714686.4 | 60.4% | 58% | (many diffs) |
| 18 | human | 6932 | TCF7 | transcription factor 7 | NM_001346450.2 | 59.6% | 58.5% | (many diffs) |
| 19 | human | 6932 | TCF7 | transcription factor 7 | XM_006714685.4 | 56.3% | 54.1% | (many diffs) |
| 20 | human | 6932 | TCF7 | transcription factor 7 | XM_011543607.2 | 54.3% | 46.9% | (many diffs) |
| 21 | human | 6932 | TCF7 | transcription factor 7 | XM_011543609.2 | 54.1% | 50.8% | (many diffs) |
| 22 | human | 6932 | TCF7 | transcription factor 7 | XM_017009790.1 | 52.8% | 43.8% | (many diffs) |
| 23 | human | 6932 | TCF7 | transcription factor 7 | XM_011543608.2 | 50.5% | 47.3% | (many diffs) |
| 24 | human | 6932 | TCF7 | transcription factor 7 | XM_011543613.3 | 40.2% | 37.7% | (many diffs) |
| 25 | human | 6932 | TCF7 | transcription factor 7 | XR_948292.1 | 34.5% | 1_204del;645_697del;1410_3338del | |
| 26 | human | 6932 | TCF7 | transcription factor 7 | NR_033449.3 | 31.5% | (many diffs) | |
| 27 | mouse | 21414 | Tcf7 | transcription factor 7, T c... | NM_001313981.1 | 58.6% | 62.2% | (many diffs) |
| 28 | mouse | 21414 | Tcf7 | transcription factor 7, T c... | NM_009331.4 | 58.6% | 62.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1221
- ORF length:
- 1152
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gccgcagctg gactccggcg ggggcggcgc gggcggcggc gacgacctcg 121 gcgcgccgga cgagctgctg gccttccagg atgaaggcga ggagcaggac gacaagagcc 181 gcgacagcgc cgccggtccc gagcgcgacc tggccgagct caagtcgtcg ctcgtgaacg 241 agtccgaggg cgcggccggc ggcgcaggga tcccgggggt cccgggggcc ggcgccgggg 301 cccgcggcga ggccgaggct ctcgggcggg aacacgctgc gcagagactc ttcccggaca 361 aacttccaga gcccctggag gacggcctga aggccccgga gtgcaccagc ggcatgtaca 421 aagagaccgt ctactccgcc ttcaatctgc tcatgcatta cccacccccc tcgggagcag 481 ggcagcaccc ccagccgcag cccccgctgc acaaggccaa tcagcccccc cacggtgtcc 541 cccaactctc tctctacgaa catttcaaca gcccacatcc cacccctgca cctgcggaca 601 tcagccagaa gcaagttcac aggcctctgc agacccctga cctctctggc ttctactccc 661 tgacctcagg cagcatgggg cagctccccc acactgtgag ctggttcacc cacccatcct 721 tgatgctagg ttctggtgta cctggtcacc cagcagccat cccccacccg gccattgtgc 781 ccccctcagg gaagcaggag ctgcagccct tcgaccgcaa cctgaagaca caagcagagt 841 ccaaggcaga gaaggaggcc aagaagccaa ccatcaagaa gcccctcaat gccttcatgc 901 tgtacatgaa ggagatgaga gccaaggtca ttgcagagtg cacacttaag gagagcgctg 961 ccatcaacca gatccTGGGC CGCAGGTGGC ACGCGCTGTC GCGAGAAGAG CAGGCCAAGT 1021 ACTATGAGCT GGCCCGCAAG GAGAGGCAGC TGCACATGCA GCTATACCCA GGCTGGTCAG 1081 CGCGGGACAA CTACGGGAAG AAGAAGAGGC GGTCGAGGGA AAAGCACCAA GAATCCACCA 1141 CAGGAGGAAA AAGAAATGCA TTCGGTACTT ACCCGGAGAA GGCCGCTGCC CCAGCCCCGT 1201 TCCTTCCGAT GACAGTGCTC TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1261 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1321 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TTGAATATCG 1381 GGTTTTTTCC AATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1441 att