Construct: ORF TRCN0000487716
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020154.1_s317c1
- DNA Barcode:
- TACTCTTTCGACAGCCAACCCGGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ARAF (369)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487716
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 369 | ARAF | A-Raf proto-oncogene, serin... | NM_001654.5 | 100% | 100% | |
| 2 | human | 369 | ARAF | A-Raf proto-oncogene, serin... | NM_001256196.1 | 99.5% | 99.5% | 459_467delGCCCTCAAG |
| 3 | human | 369 | ARAF | A-Raf proto-oncogene, serin... | NM_001256197.1 | 30.6% | 30.5% | 558_558delGins1261 |
| 4 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | NM_009703.2 | 89.9% | 95.2% | (many diffs) |
| 5 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | XM_006527556.3 | 89.9% | 95.2% | (many diffs) |
| 6 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | XM_006527557.3 | 89.9% | 95.2% | (many diffs) |
| 7 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | XM_011247429.2 | 84.8% | 89.7% | (many diffs) |
| 8 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | XM_011247430.2 | 67.1% | 69.1% | (many diffs) |
| 9 | mouse | 11836 | Araf | Araf proto-oncogene, serine... | NM_001159645.1 | 27.7% | 29% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1890
- ORF length:
- 1818
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagcca ccacggggcc cccctgccaa tggggccgag ccatcccggg 121 cagtgggcac cgtcaaagta tacctgccca acaagcaacg cacggtggtg actgtccggg 181 atggcatgag tgtctacgac tctctagaca aggccctgaa ggtgcggggt ctaaatcagg 241 actgctgtgt ggtctaccga ctcatcaagg gacgaaagac ggtcactgcc tgggacacag 301 ccattgctcc cctggatggc gaggagctca ttgtcgaggt ccttgaagat gtcccgctga 361 ccatgcacaa ttttgtacgg aagaccttct tcagcctggc gttctgtgac ttctgcctta 421 agtttctgtt ccatggcttc cgttgccaaa cctgtggcta caagttccac cagcattgtt 481 cctccaaggt ccccacagtc tgtgttgaca tgagtaccaa ccgccaacag ttctaccaca 541 gtgtccagga tttgtccgga ggctccagac agcatgaggc tccctcgaac cgccccctga 601 atgagttgct aaccccccag ggtcccagcc cccgcaccca gcactgtgac ccggagcact 661 tccccttccc tgccccagcc aatgcccccc tacagcgcat ccgctccacg tccactccca 721 acgtccatat ggtcagcacc acggccccca tggactccaa cctcatccag ctcactggcc 781 agagtttcag cactgatgct gccggtagta gaggaggtag tgatggaacc ccccggggga 841 gccccagccc agccagcgtg tcctcgggga ggaagtcccc acattccaag tcaccagcag 901 agcagcgcga gcggaagtcc ttggccgatg acaagaagaa agtgaagaac ctggggtacc 961 gggactcagg ctattactgg gaggtaccac ccagtgaggt gcagctgctg aagaggatcg 1021 ggacgggctc gtttggcacc gtgtttcgag ggcggtggca tggcgatgtg gccgtgaagg 1081 tgctcaaggt gtcccagccc acagctgagc aggcccaggc tttcaagaat gagatgcagg 1141 tgctcaggaa gacgcgacat gtcaacatct tgctgtttat gggcttcatg acccggccgg 1201 gatttgccat catcacacag tggtgtgagg gctccagcct ctaccatcac ctgcatgtgg 1261 ccgacacacg cttcgacatg gtccagctca tcgacgtggc ccggcagact gcccagggca 1321 tggactacct ccatgccaag aacatcatcc accgagatct caagtctaac aacatcttcc 1381 tacatgaggg gctcacggtg aagatcggtg actttggctt ggccacagtg aagactcgat 1441 ggagcggggc ccagcccttg gagcagccct caggatctgt gctgtggatg gcagctgagg 1501 tgatccgtat gcaggacccg aacccctaca gcttccagtc agacgtctat gcctacgggg 1561 ttgtgctcta cgagcttatg actggctcac tgccttacag ccacattggc tgccgtgacc 1621 agattatctt tatggtgggc cgtggctatc tgtccccgga ccTCAGCAAA ATCTCCAGCA 1681 ACTGCCCCAA GGCCATGCGG CGCCTGCTGT CTGACTGCCT CAAGTTCCAG CGGGAGGAGC 1741 GGCCCCTCTT CCCCCAGATC CTGGCCACAA TTGAGCTGCT GCAACGGTCA CTCCCCAAGA 1801 TTGAGCGGAG TGCCTCGGAA CCCTCCTTGC ACCGCACCCA GGCCGATGAG TTGCCTGCCT 1861 GCCTACTCAG CGCAGCCCGC CTTGTGCCTT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 1921 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1981 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 2041 CTCTTTCGAC AGCCAACCCG GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 2101 ttgtgaaaga tt