Construct: ORF TRCN0000487729
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021100.1_s317c1
- DNA Barcode:
- TGGGCGTCAGCGAGTCGAATAAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NPR2 (4882)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487729
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4882 | NPR2 | natriuretic peptide receptor 2 | NM_003995.3 | 100% | 100% | |
| 2 | human | 4882 | NPR2 | natriuretic peptide receptor 2 | XM_005251478.3 | 99.7% | 99.7% | 1436_1444delGTCCTTACA |
| 3 | human | 4882 | NPR2 | natriuretic peptide receptor 2 | XM_024447557.1 | 95.1% | 95% | 2048_2206del |
| 4 | human | 4882 | NPR2 | natriuretic peptide receptor 2 | XM_024447556.1 | 94.9% | 94.8% | 1436_1444delGTCCTTACA;2057_2215del |
| 5 | human | 4882 | NPR2 | natriuretic peptide receptor 2 | XM_024447558.1 | 65% | 65% | 0_1ins987;449_457delGTCCTTACA;1070_1228del |
| 6 | human | 4882 | NPR2 | natriuretic peptide receptor 2 | XM_024447561.1 | 55.3% | 55.3% | 0_1ins1404 |
| 7 | human | 4882 | NPR2 | natriuretic peptide receptor 2 | XM_024447560.1 | 52.6% | 52.5% | 0_1ins1404;644_802del |
| 8 | human | 4882 | NPR2 | natriuretic peptide receptor 2 | XM_024447559.1 | 52.4% | 52.4% | 0_1ins1404;32_40delGTCCTTACA;653_811del |
| 9 | mouse | 230103 | Npr2 | natriuretic peptide receptor 2 | NM_173788.4 | 92.2% | 98.6% | (many diffs) |
| 10 | mouse | 230103 | Npr2 | natriuretic peptide receptor 2 | NM_001355466.1 | 89.8% | 96.2% | (many diffs) |
| 11 | mouse | 230103 | Npr2 | natriuretic peptide receptor 2 | XR_390318.4 | 43.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3213
- ORF length:
- 3141
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgctg ccatcacttc tgctgttggt ggcagccctg gcaggtgggg 121 tgcgtcctcc cggggcgcgg aacctgacgc tggcggtggt gctgccagaa cacaacctga 181 gctatgcctg ggcctggcca cgggtgggac ccgctgtggc actagctgtg gaggctctgg 241 gccgggcact gcccgtggac ctgcggtttg tcagctccga actggaaggc gcctgctctg 301 agtacctggc accgctgagc gctgtggacc tcaagctgta ccatgacccc gacctgctgt 361 taggtcccgg ttgcgtgtac cctgctgcct ctgtggcccg ctttgcctcc cactggcgcc 421 ttcccctgct gactgcgggt gctgtggcct ctggtttttc ggctaagaat gaccattatc 481 gtaccctggt tcgcactggc ccctctgctc ccaagctggg tgagtttgtg gtgacactac 541 acgggcactt caattggact gcccgtgctg ccttgctgta cctggatgct cgcacagatg 601 accggcctca ctacttcacc atcgagggcg tctttgaggc cctgcagggc agcaacctca 661 gtgtgcagca ccaggtgtat gcccgagagc cagggggccc cgagcaggcc acccacttca 721 tccgggccaa cgggcgcatt gtgtatatct gcggccctct ggagatgctg catgagatcc 781 tgcttcaggc ccagagggag aatctgacca atggggatta tgtcttcttt tacctggatg 841 tctttgggga gagtctccgt gcaggcccca cacgtgctac aggccggccc tggcaggaca 901 atcgcacccg ggaacaggcc caggccctca gagaggcctt tcagactgta ttggtgatca 961 cgtaccgaga acccccaaat cctgagtatc aggaattcca gaatcgtctg ctgataagag 1021 cccgggaaga ctttggtgtg gagctgggcc cttccctgat gaacctcatc gctggctgct 1081 tctatgatgg gatcctgcta tatgctgaag tcctgaatga gacaatacag gaaggaggca 1141 cccgggagga tggacttcga attgtggaaa agatgcaggg acgaagatat cacggtgtaa 1201 ctgggctggt tgtcatggac aagaacaatg accgagagac tgactttgtc ctctgggcca 1261 tgggagacct ggattctggg gactttcagc ctgcagccca ctactcggga gctgagaagc 1321 agatttggtg gacgggacgg cctattccct gggtgaaggg ggctcctccc tcggacaatc 1381 ccccctgtgc ctttgacttg gacgacccat cctgtgataa aactccactt tcaaccctgg 1441 caattgtggc tctgggcaca ggaatcacct tcatcatgtt tggtgtttcc agcttcctaa 1501 ttttccgaaa gctgatgctg gagaaggagc tggctagcat gttgtggcgt attcgctggg 1561 aagaactgca gtttggcaac tcagagcgtt atcacaaagg tgcaggcagt cgcctcacac 1621 tgtcgctgcg gggatccagt tacggctcgc tcatgacagc ccatgggaaa taccagatct 1681 ttgccaacac cggtcacttc aagggaaatg ttgtcgccat caaacatgtg aataagaagc 1741 gcattgagct gacccggcag gttctgtttg aactcaaaca tatgagagat gttcagttca 1801 accatctcac tcgcttcatt ggcgcctgca tagaccctcc caacatttgc attgtcactg 1861 aatactgtcc tcgtgggagt ttacaggata ttctagaaaa tgacagcatc aacttggact 1921 ggatgtttcg ttattcactc attaatgacc ttgttaaggg catggccttt ctccacaaca 1981 gcattatttc atcgcatggg agtctcaagt cctccaactg tgtggtggat agtcgttttg 2041 tgctcaaaat cacagactat ggcctggcca gcttccgatc aactgctgaa cctgatgaca 2101 gccatgccct ctatgccaag aagctgtgga ctgccccaga actgctcagt gggaacccct 2161 tgccaaccac aggcatgcag aaggctgacg tctatagctt tgggatcatc ctgcaggaga 2221 tagcacttcg cagtggtcct ttctacttgg agggcctgga cctcagcccc aaagagattg 2281 tccagaaggt acgaaatggt cagcggccat atttccggcc aagcattgac cggacccaac 2341 tgaatgaaga gctagttttg ctgatggagc gatgttgggc tcaggaccca gctgagcggc 2401 cagactttgg acagattaag ggcttcattc ggcgctttaa caaggagggt ggcaccagca 2461 tattggacaa cctcctgctg cgcatggaac agtatgccaa taacttggag aagctggtgg 2521 aggaacgcac acaggcctat ctggaggaaa aacgcaaggc tgaagctctg ctctaccaaa 2581 tcctacccca ttcagtggca gagcagttaa aacggggaga gactgtacag gctgaggcct 2641 ttgacagtgt taccatctac ttcagtgaca ttgttggctt cacagcattg tcagcagaga 2701 gcacccccat gcaggtagtg acacttctta atgacctgta tacctgcttt gatgccataa 2761 ttgacaactt tgatgtctac aaggtggaga cgattgggga tgcttacatg gtggtatctg 2821 gcctcccagg ccgaaatggt caacgccatg caccagaaat tgctcgtatg gccctagcat 2881 tactagatgc agtttcttcc tttcgcatcc gccaccgacc ccatgaccag ctgaggctac 2941 gcataggggt ccatactggg ccagtctgtg ctggggttgt tggcctgaag atgccccgtt 3001 attgtctttt tGGAGACACA GTGAACACTG CTTCTCGAAT GGAGTCTAAT GGTCAAGCGC 3061 TGAAGATCCA TGTCTCCTCT ACCACCAAGG ATGCCCTAGA TGAGCTAGGA TGCTTCCAGC 3121 TAGAGCTTCG GGGGGATGTG GAAATGAAGG GAAAAGGAAA GATGCGAACA TACTGGCTCT 3181 TAGGAGAGCG GAAAGGACCT CCTGGACTCC TGTAAGACCC AGCTTTCTTG TACAAAGTGG 3241 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3301 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3361 ATGGGCGTCA GCGAGTCGAA TAAACACGCG TTAAGTCgac aatcaacctc tggattacaa 3421 aatttgtgaa agatt