Construct: ORF TRCN0000487737
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021097.1_s317c1
- DNA Barcode:
- ATTGGGATACTGGTGTTTCGATTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- APLNR (187)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487737
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 187 | APLNR | apelin receptor | NM_005161.4 | 100% | 100% | |
| 2 | human | 187 | APLNR | apelin receptor | NR_027991.2 | 66% | 1_246del;1387_1727del | |
| 3 | mouse | 23796 | Aplnr | apelin receptor | NM_011784.3 | 86.9% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1212
- ORF length:
- 1140
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaggaa ggtggtgatt ttgacaacta ctatggggca gacaaccagt 121 ctgagtgtga gtacacagac tggaaatcct cgggggccct catccctgcc atctacatgt 181 tggtcttcct cctgggcacc acgggcaacg gtctggtgct ctggaccgtg tttcggagca 241 gccgggagaa gaggcgctca gctgatatct tcattgctag cctggcggtg gctgacctga 301 ccttcgtggt gacgctgccc ctgtgggcta cctacacgta ccgggactat gactggccct 361 ttgggacctt cttctgcaag ctcagcagct acctcatctt cgtcaacatg tacgccagcg 421 tcttctgcct caccggcctc agcttcgacc gctacctggc catcgtgagg ccagtggcca 481 atgctcggct gaggctgcgg gtcagcgggg ccgtggccac ggcagttctt tgggtgctgg 541 ccgccctcct ggccatgcct gtcatggtgt tacgcaccac cggggacttg gagaacacca 601 ctaaggtgca gtgctacatg gactactcca tggtggccac tgtgagctca gagtgggcct 661 gggaggtggg ccttggggtc tcgtccacca ccgtgggctt tgtggtgccc ttcaccatca 721 tgctgacctg ttacttcttc atcgcccaaa ccatcgctgg ccacttccgc aaggaacgca 781 tcgagggcct gcggaagcgg cgccggctgc tcagcatcat cgtggtgctg gtggtgacct 841 ttgccctgtg ctggatgccc taccacctgg tgaagacgct gtacatgctg ggcagcctgc 901 tgcactggcc ctgtgacttt gacctcttcc tcatgaacat cttccccTAC TGCACCTGCA 961 TCAGCTACGT CAACAGCTGC CTCAACCCCT TCCTCTATGC CTTTTTCGAC CCCCGCTTCC 1021 GCCAGGCCTG CACCTCCATG CTCTGCTGTG GCCAGAGCAG GTGCGCAGGC ACCTCCCACA 1081 GCAGCAGTGG GGAGAAGTCA GCCAGCTACT CTTCGGGGCA CAGCCAGGGG CCCGGCCCCA 1141 ACATGGGCAA GGGTGGAGAA CAGATGCACG AGAAATCCAT CCCCTACAGC CAGGAGACCC 1201 TTGTGGTTGA CTAGGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1261 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1321 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATTGGGATAC TGGTGTTTCG 1381 ATTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt