Construct: ORF TRCN0000487765
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019255.1_s317c1
- DNA Barcode:
- TTCTTGTGTCTAGGATACTCTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOMER2 (9455)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487765
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9455 | HOMER2 | homer scaffold protein 2 | NM_004839.4 | 100% | 100% | |
| 2 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_005272449.4 | 99.9% | 99.7% | 4A>G |
| 3 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_005272448.5 | 97.1% | 96.6% | 1_12del;17_34del |
| 4 | human | 9455 | HOMER2 | homer scaffold protein 2 | NM_199330.3 | 96.8% | 96.8% | 388_420del |
| 5 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_006720776.4 | 96.7% | 96.6% | 4A>G;388_420del |
| 6 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_011522233.3 | 94.6% | 94.7% | 1_23delinsA;27_28delAA;412_444del |
| 7 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_006720775.3 | 94.2% | 93.6% | 1_12del;17_34del;418_450del |
| 8 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_011522231.3 | 94.2% | 93.6% | 1_12del;17_34del;418_450del |
| 9 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_011522232.2 | 94.2% | 93.6% | 1_12del;17_34del;418_450del |
| 10 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_011522234.3 | 90.1% | 90.1% | 0_1ins72;316_348del |
| 11 | mouse | 26557 | Homer2 | homer scaffolding protein 2 | NM_001164086.1 | 89.8% | 94.4% | (many diffs) |
| 12 | mouse | 26557 | Homer2 | homer scaffolding protein 2 | NM_011983.2 | 87% | 91.5% | (many diffs) |
| 13 | mouse | 26557 | Homer2 | homer scaffolding protein 2 | NM_001164087.1 | 70.2% | 74% | (many diffs) |
| 14 | mouse | 26557 | Homer2 | homer scaffolding protein 2 | XM_006540909.1 | 70.2% | 74% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1104
- ORF length:
- 1029
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggga gaacagccca tcttcaccac ccgagcgcat gtcttccaga 121 ttgaccccaa caccaagaag aactggatgc ctgcgagcaa gcaggcggtc accgtttcct 181 acttctatga tgtcacaagg aacagctatc ggatcatcag tgtggacgga gccaaggtga 241 tcataaacag cacaatcaca ccgaatatga ccttcaccaa aacgtcacag aagtttgggc 301 agtgggccga cagcagagcc aacacagtgt ttggtttggg gttttcctct gagcagcagc 361 tgacaaagtt tgcagagaaa ttccaggagg tgaaagaagc tgccaagata gccaaagaca 421 agacgcagga gaaaatcgag acctcaagta atcattccca agcatccagt gtcaacggga 481 cggacgatga aaaggcctct cacgccggtc cagccaacac acacctgaag tctgagaatg 541 acaagctgaa gattgccttg acgcagagcg cagccaacgt gaagaagtgg gagatcgagc 601 tgcagaccct tcgggagagc aatgcacggc tgaccacagc actgcaggag tcggcagcca 661 gtgtggagca gtggaagagg cagttctcca tctgccgtga tgagaatgac cggctccgca 721 acaagattga tgagctggaa gaacaatgca gtgagatcaa cagagagaag gagaagaaca 781 cgcagctgaa gaggaggatc gaggagctgg aggcagagct ccgagaaaag gagacagagc 841 tgaaagatct ccgaaaacaa agtgaaatca taccTCAGCT CATGTCAGAG TGCGAATATG 901 TCTCTGAGAA GCTAGAGGCG GCAGAGAGAG ACAATCAAAA CCTGGAAGAC AAAGTGCGTT 961 CCTTAAAGAC AGACATTGAG GAGAGCAAAT ACCGACAGCG CCACCTGAAG GTGGAGTTGA 1021 AGAGCTTCCT GGAGGTGCTG GACGGGAAGA TTGACGACCT GCATGACTTC CGCCGAGGGC 1081 TCTCCAAGCT GGGCACCGAT AACGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1141 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1201 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TCTTGTGTCT 1261 AGGATACTCT TTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1321 att