Construct: ORF TRCN0000487775
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021857.1_s317c1
- DNA Barcode:
- GTTCAACGCCCGGGTGCCGGACCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KRT18 (3875)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487775
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3875 | KRT18 | keratin 18 | NM_000224.3 | 100% | 100% | |
| 2 | human | 3875 | KRT18 | keratin 18 | NM_199187.1 | 100% | 100% | |
| 3 | human | 284085 | KRT18P55 | keratin 18 pseudogene 55 | NR_028334.1 | 59.6% | (many diffs) | |
| 4 | human | 693207 | MIR622 | microRNA 622 | NR_030754.1 | 7% | (many diffs) | |
| 5 | mouse | 16668 | Krt18 | keratin 18 | NM_010664.2 | 83.1% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1359
- ORF length:
- 1290
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gagcttcacc actcgctcca ccttctccac caactaccgg tccctgggct 121 ctgtccaggc gcccagctac ggcgcccggc cggtcagcag cgcggccagc gtctatgcag 181 gcgctggggg ctctggttcc cggatctccg tgtcccgctc caccagcttc aggggcggca 241 tggggtccgg gggcctggcc accgggatag ccgggggtct ggcaggaatg ggaggcatcc 301 agaacgagaa ggagaccatg caaagcctga acgaccgcct ggcctcttac ctggacagag 361 tgaggagcct ggagaccgag aaccggaggc tggagagcaa aatccgggag cacttggaga 421 agaagggacc ccaggtcaga gactggagcc attacttcaa gatcatcgag gacctgaggg 481 ctcagatctt cgcaaatact gtggacaatg cccgcatcgt tctgcagatt gacaatgccc 541 gtcttgctgc tgatgacttt agagtcaagt atgagacaga gctggccatg cgccagtctg 601 tggagaacga catccatggg ctccgcaagg tcattgatga caccaatatc acacgactgc 661 agctggagac agagatcgag gctctcaagg aggagctgct cttcatgaag aagaaccacg 721 aagaggaagt aaaaggccta caagcccaga ttgccagctc tgggttgacc gtggaggtag 781 atgcccccaa atctcaggac ctcgccaaga tcatggcaga catccgggcc caatatgacg 841 agctggctcg gaagaaccga gaggagctag acaagtactg gtctcagcag attgaggaga 901 gcaccacagt ggtcaccaca cagtctgctg aggttggagc tgctgagacg acgctcacag 961 agctgagacg tacagtccag tccttggaga tcgacctgga ctccatgaga aatctgaagg 1021 ccagcttgga gaacagcctg agggaggtgg aggcccgcta cgccctacag atggagcagc 1081 tcaacgggat ccTGCTGCAC CTTGAGTCAG AGCTGGCACA GACCCGGGCA GAGGGACAGC 1141 GCCAGGCCCA GGAGTATGAG GCCCTGCTGA ACATCAAGGT CAAGCTGGAG GCTGAGATCG 1201 CCACCTACCG CCGCCTGCTG GAAGATGGCG AGGACTTTAA TCTTGGTGAT GCCTTGGACA 1261 GCAGCAACTC CATGCAAACC ATCCAAAAGA CCACCACCCG CCGGATAGTG GATGGCAAAG 1321 TGGTGTCTGA GACCAATGAC ACCAAAGTTC TGAGGCATTA GAACCCAGCT TTCTTGTACA 1381 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1441 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1501 AGGACGAGTT CAACGCCCGG GTGCCGGACC CACGCGTTAA GTCgacaatc aacctctgga 1561 ttacaaaatt tgtgaaagat t