Construct: ORF TRCN0000487776
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021681.1_s317c1
- DNA Barcode:
- ACGTTGCACTGGCGGACTCGCCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRIN1 (2902)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487776
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2902 | GRIN1 | glutamate ionotropic recept... | NM_007327.4 | 100% | 100% | |
| 2 | human | 2902 | GRIN1 | glutamate ionotropic recept... | XM_005266073.4 | 97.8% | 97.8% | 566_628del |
| 3 | human | 2902 | GRIN1 | glutamate ionotropic recept... | XM_005266071.3 | 96.9% | 95.5% | (many diffs) |
| 4 | human | 2902 | GRIN1 | glutamate ionotropic recept... | NM_021569.4 | 96% | 96% | 2588_2589ins111 |
| 5 | human | 2902 | GRIN1 | glutamate ionotropic recept... | NM_001185090.2 | 94.8% | 93.4% | (many diffs) |
| 6 | human | 2902 | GRIN1 | glutamate ionotropic recept... | XM_005266072.3 | 93.9% | 93.9% | 566_628del;2651_2652ins111 |
| 7 | human | 2902 | GRIN1 | glutamate ionotropic recept... | NM_000832.7 | 93.4% | 91.8% | (many diffs) |
| 8 | human | 2902 | GRIN1 | glutamate ionotropic recept... | NM_001185091.2 | 91.4% | 89.8% | (many diffs) |
| 9 | human | 2902 | GRIN1 | glutamate ionotropic recept... | XM_011518583.2 | 90.7% | 90.3% | (many diffs) |
| 10 | mouse | 14810 | Grin1 | glutamate receptor, ionotro... | NM_008169.3 | 90.7% | 99% | (many diffs) |
| 11 | mouse | 14810 | Grin1 | glutamate receptor, ionotro... | XM_006497722.3 | 88.7% | 96.8% | (many diffs) |
| 12 | mouse | 14810 | Grin1 | glutamate receptor, ionotro... | XM_006497725.3 | 87.6% | 94.6% | (many diffs) |
| 13 | mouse | 14810 | Grin1 | glutamate receptor, ionotro... | XM_006497726.2 | 86.8% | 95% | (many diffs) |
| 14 | mouse | 14810 | Grin1 | glutamate receptor, ionotro... | XM_006497723.2 | 85.7% | 92.5% | (many diffs) |
| 15 | mouse | 14810 | Grin1 | glutamate receptor, ionotro... | XM_006497724.3 | 84.9% | 93% | (many diffs) |
| 16 | mouse | 14810 | Grin1 | glutamate receptor, ionotro... | NM_001177657.2 | 84.2% | 90.8% | (many diffs) |
| 17 | mouse | 14810 | Grin1 | glutamate receptor, ionotro... | NM_001177656.2 | 82.4% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2883
- ORF length:
- 2814
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gagcaccatg cgcctgctga cgctcgccct gctgttctcc tgctccgtcg 121 cccgtgccgc gtgcgacccc aagatcgtca acattggcgc ggtgctgagc acgcggaagc 181 acgagcagat gttccgcgag gccgtgaacc aggccaacaa gcggcacggc tcctggaaga 241 ttcagctcaa tgccacctcc gtcacgcaca agcccaacgc catccagatg gctctgtcgg 301 tgtgcgagga cctcatctcc agccaggtct acgccatcct agttagccat ccacctaccc 361 ccaacgacca cttcactccc acccctgtct cctacacagc cggcttctac cgcatacccg 421 tgctggggct gaccacccgc atgtccatct actcggacaa gagcatccac ctgagcttcc 481 tgcgcaccgt gccgccctac tcccaccagt ccagcgtgtg gtttgagatg atgcgtgtct 541 acagctggaa ccacatcatc ctgctggtca gcgacgacca cgagggccgg gcggctcaga 601 aacgcctgga gacgctgctg gaggagcgtg agtccaaggc agagaaggtg ctgcagtttg 661 acccagggac caagaacgtg acggccctgc tgatggaggc gaaagagctg gaggcccggg 721 tcatcatcct ttctgccagc gaggacgatg ctgccactgt ataccgcgca gccgcgatgc 781 tgaacatgac gggctccggg tacgtgtggc tggtcggcga gcgcgagatc tcggggaacg 841 ccctgcgcta cgccccagac ggcatcctcg ggctgcagct catcaacggc aagaacgagt 901 cggcccacat cagcgacgcc gtgggcgtgg tggcccaggc cgtgcacgag ctcctcgaga 961 aggagaacat caccgacccg ccgcggggct gcgtgggcaa caccaacatc tggaagaccg 1021 ggccgctctt caagagagtg ctgatgtctt ccaagtatgc ggatggggtg actggtcgcg 1081 tggagttcaa tgaggatggg gaccggaagt tcgccaacta cagcatcatg aacctgcaga 1141 accgcaagct ggtgcaagtg ggcatctaca atggcaccca cgtcatccct aatgacagga 1201 agatcatctg gccaggcgga gagacagaga agcctcgagg gtaccagatg tccaccagac 1261 tgaagattgt gacgatccac caggagccct tcgtgtacgt caagcccacg ctgagtgatg 1321 ggacatgcaa ggaggagttc acagtcaacg gcgacccagt caagaaggtg atctgcaccg 1381 ggcccaacga cacgtcgccg ggcagccccc gccacacggt gcctcagtgt tgctacggct 1441 tttgcatcga cctgctcatc aagctggcac ggaccatgaa cttcacctac gaggtgcacc 1501 tggtggcaga tggcaagttc ggcacacagg agcgggtgaa caacagcaac aagaaggagt 1561 ggaatgggat gatgggcgag ctgctcagcg ggcaggcaga catgatcgtg gcgccgctaa 1621 ccataaacaa cgagcgcgcg cagtacatcg agttttccaa gcccttcaag taccagggcc 1681 tgactattct ggtcaagaag gagattcccc ggagcacgct ggactcgttc atgcagccgt 1741 tccagagcac actgtggctg ctggtggggc tgtcggtgca cgtggtggcc gtgatgctgt 1801 acctgctgga ccgcttcagc cccttcggcc ggttcaaggt gaacagcgag gaggaggagg 1861 aggacgcact gaccctgtcc tcggccatgt ggttctcctg gggcgtcctg ctcaactccg 1921 gcatcgggga aggcgccccc agaagcttct cagcgcgcat cctgggcatg gtgtgggccg 1981 gctttgccat gatcatcgtg gcctcctaca ccgccaacct ggcggccttc ctggtgctgg 2041 accggccgga ggagcgcatc acgggcatca acgaccctcg gctgaggaac ccctcggaca 2101 agtttatcta cgccacggtg aagcagagct ccgtggatat ctacttccgg cgccaggtgg 2161 agctgagcac catgtaccgg catatggaga agcacaacta cgagagtgcg gcggaggcca 2221 tccaggccgt gagagacaac aagctgcatg ccttcatctg ggactcggcg gtgctggagt 2281 tcgaggcctc gcagaagtgc gacctggtga cgactggaga gctgtttttc cgctcgggct 2341 tcggcatagg catgcgcaaa gacagcccct ggaagcagaa cgtctccctg tccatcctca 2401 agtcccacga gaatggcttc atggaagacc tggacaagac gtgggttcgg tatcaggaat 2461 gtgactcgcg cagcaacgcc cctgcgaccc ttacttttga gaacatggcc ggggtcttca 2521 tgctggtagc tgggggcatc gtggccggga tcttcctgat tttcatcgag attgccTACA 2581 AGCGGCACAA GGATGCTCGC CGGAAGCAGA TGCAGCTGGC CTTTGCCGCC GTTAACGTGT 2641 GGCGGAAGAA CCTGCAGGAT AGAAAGAGTG GTAGAGCAGA GCCTGACCCT AAAAAGAAAG 2701 CCACATTTAG GGCTATCACC TCCACCCTGG CTTCCAGCTT CAAGAGGCGT AGGTCCTCCA 2761 AAGACACGAG CACCGGGGGT GGACGCGGCG CTTTGCAAAA CCAAAAAGAC ACAGTGCTGC 2821 CGCGACGCGC TATTGAGAGG GAGGAGGGCC AGCTGCAGCT GTGTTCCCGT CATAGGGAGA 2881 GCTGAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2941 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 3001 CTTGGCTTTA TATATCTTGT GGAAAGGACG AACGTTGCAC TGGCGGACTC GCCTTACGCG 3061 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt