Construct: ORF TRCN0000487809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020305.1_s317c1
- DNA Barcode:
- ACCGGCGGAAATGCTTGCAGCGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- IKBKE (9641)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487809
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9641 | IKBKE | inhibitor of nuclear factor... | NM_014002.4 | 99.9% | 100% | 201T>C |
| 2 | human | 9641 | IKBKE | inhibitor of nuclear factor... | XM_005273356.2 | 99.9% | 100% | 201T>C |
| 3 | human | 9641 | IKBKE | inhibitor of nuclear factor... | NM_001193322.2 | 91.7% | 90.5% | 201T>C;1932_1933ins113;1971_1972ins64 |
| 4 | human | 9641 | IKBKE | inhibitor of nuclear factor... | NM_001193321.2 | 88.1% | 88.1% | 0_1ins255 |
| 5 | human | 9641 | IKBKE | inhibitor of nuclear factor... | XM_017002868.1 | 82.9% | 82.9% | 0_1ins366 |
| 6 | human | 9641 | IKBKE | inhibitor of nuclear factor... | XR_922002.2 | 65.5% | (many diffs) | |
| 7 | mouse | 56489 | Ikbke | inhibitor of kappaB kinase ... | XM_006529762.3 | 66.4% | 68.1% | (many diffs) |
| 8 | mouse | 56489 | Ikbke | inhibitor of kappaB kinase ... | XM_006529763.3 | 59% | 59.7% | (many diffs) |
| 9 | mouse | 56489 | Ikbke | inhibitor of kappaB kinase ... | XM_006529764.3 | 57.9% | 58.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2220
- ORF length:
- 2148
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcagagc acagccaatt acctgtggca cacagatgac ctgctggggc 121 agggggccac tgccagtgtg tacaaggccc gcaacaagaa atccggagag ctggttgctg 181 tgaaggtctt caacactacc agctacctgc ggccccgcga ggtgcaggtg agggagtttg 241 aggtcctgcg gaagctgaac caccagaaca tcgtcaagct ctttgcggtg gaggagacgg 301 gcggaagccg gcagaaggta ctggtgatgg agtactgctc cagtgggagc ctgctgagtg 361 tgctggagag ccctgagaat gcctttgggc tgcctgagga tgagttcctg gtggtgctgc 421 gctgtgtggt ggccggcatg aaccacctgc gggagaacgg cattgtgcat cgcgacatca 481 agccggggaa catcatgcgc ctcgtagggg aggaggggca gagcatctac aagctgacag 541 acttcggcgc tgcccgggag ctggatgatg atgagaagtt cgtctcggtc tatgggactg 601 aggagtacct gcatcccgac atgtatgagc gggcggtgct tcgaaagccc cagcaaaaag 661 cgttcggggt gactgtggat ctctggagca ttggagtgac cttgtaccat gcagccactg 721 gcagcctgcc cttcatcccc tttggtgggc cacggcggaa caaggagatc atgtaccgga 781 tcaccacgga gaagccggct ggggccattg caggtgccca gaggcgggag aacgggcccc 841 tggagtggag ctacaccctc cccatcacct gccagctgtc actggggctg cagagccagc 901 tggtgcccat cctggccaac atcctggagg tggagcaggc caagtgctgg ggcttcgacc 961 agttctttgc ggagaccagt gacatcctgc agcgagttgt cgtccatgtc ttctccctgt 1021 cccaggcagt cctgcaccac atctatatcc atgcccacaa cacgatagcc attttccagg 1081 aggccgtgca caagcagacc agtgtggccc cccgacacca ggagtacctc tttgagggtc 1141 acctctgtgt cctcgagccc agcgtctcag cacagcacat cgcccacacg acggcaagca 1201 gccccctgac cctcttcagc acagccatcc ctaaggggct ggccttcagg gaccctgctc 1261 tggacgtccc caagttcgtc cccaaagtgg acctgcaggc ggattacaac actgccaagg 1321 gcgtgttggg cgccggctac caggccctgc ggctggcacg ggccctgctg gatgggcagg 1381 agctaatgtt tcgggggctg cactgggtca tggaggtgct ccaggccaca tgcagacgga 1441 ctctggaagt ggcaaggaca tccctcctct acctcagcag cagcctggga actgagaggt 1501 tcagcagcgt ggctggaacg cctgagatcc aggaactgaa ggcggctgca gaactgaggt 1561 ccaggctgcg gactctagcg gaggtcctct ccagatgctc ccaaaatatc acggagaccc 1621 aggagagcct gagcagcctg aaccgggagc tggtgaagag ccgggatcag gtacatgagg 1681 acagaagcat ccagcagatt cagtgctgtt tggacaagat gaacttcatc tacaaacagt 1741 tcaagaagtc taggatgagg ccagggcttg gctacaacga ggagcagatt cacaagctgg 1801 ataaggtgaa tttcagtcat ttagccaaaa gactcctgca ggtgttccag gaggagtgcg 1861 tgcagaagta tcaagcgtcc ttagtcacac acggcaagag gatgagggtg gtgcacgaga 1921 ccaggaacca ccTGCGCCTG GTTGGCTGTT CTGTGGCTGC CTGTAACACA GAAGCCCAGG 1981 GGGTCCAGGA GAGTCTCAGC AAGCTCCTGG AAGAGCTATC TCACCAGCTC CTTCAGGACC 2041 GAGCAAAGGG GGCTCAGGCC TCGCCGCCTC CCATAGCTCC TTACCCCAGC CCTACACGAA 2101 AGGACCTGCT TCTCCACATG CAAGAGCTCT GCGAGGGGAT GAAGCTGCTG GCATCTGACC 2161 TCCTGGACAA CAACCGCATC ATCGAACGGC TAAATAGAGT CCCAGCACCT CCTGATGTCT 2221 GAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2281 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2341 GGCTTTATAT ATCTTGTGGA AAGGACGAAC CGGCGGAAAT GCTTGCAGCG TCACGCGTTA 2401 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt