Construct: ORF TRCN0000487819
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021230.1_s317c1
- DNA Barcode:
- TTAACACTCCCCGGCGCAGTGGAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NPR1 (4881)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487819
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4881 | NPR1 | natriuretic peptide receptor 1 | NM_000906.4 | 99.9% | 100% | 2679A>G |
| 2 | human | 4881 | NPR1 | natriuretic peptide receptor 1 | XM_005245218.2 | 97.5% | 97.5% | 1680_1681ins78;2601A>G |
| 3 | human | 4881 | NPR1 | natriuretic peptide receptor 1 | XM_017001374.2 | 66.2% | 65.9% | (many diffs) |
| 4 | mouse | 18160 | Npr1 | natriuretic peptide receptor 1 | NM_008727.5 | 86.4% | 90.8% | (many diffs) |
| 5 | mouse | 18160 | Npr1 | natriuretic peptide receptor 1 | XR_375494.1 | 49.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3255
- ORF length:
- 3183
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgccgggg ccccggcgcc ccgctggctc ccgcctgcgc ctgctcctgc 121 tcctgctgct gccgccgctg ctgctgctgc tccggggcag ccacgcgggc aacctgacgg 181 tagccgtggt actgccgctg gccaatacct cgtacccctg gtcgtgggcg cgcgtgggac 241 ccgccgtgga gctggccctg gcccaggtga aggcgcgccc cgacttgctg ccgggctgga 301 cggtccgcac ggtgctgggc agcagcgaaa acgcgctggg cgtctgctcc gacaccgcag 361 cgcccctggc cgcggtggac ctcaagtggg agcacaaccc cgctgtgttc ctgggccccg 421 gctgcgtgta cgccgccgcc ccagtggggc gcttcaccgc gcactggcgg gtcccgctgc 481 tgaccgccgg cgccccggcg ctgggcttcg gtgtcaagga cgagtatgcg ctgaccaccc 541 gcgcggggcc cagctacgcc aagctggggg acttcgtggc ggcgctgcac cgacggctgg 601 gctgggagcg ccaagcgctc atgctctacg cctaccggcc gggtgacgaa gagcactgct 661 tcttcctcgt ggaggggctg ttcatgcggg tccgcgaccg cctcaatatt acggtggacc 721 acctggagtt cgccgaggac gacctcagcc actacaccag gctgctgcgg accatgccgc 781 gcaaaggccg agttatctac atctgcagct cccctgatgc cttcagaacc ctcatgctcc 841 tggccctgga agctggcttg tgtggggagg actacgtttt cttccacctg gatatctttg 901 ggcaaagcct gcaaggtgga cagggccctg ctccccgcag gccctgggag agaggggatg 961 ggcaggatgt cagtgcccgc caggcctttc aggctgccaa aatcattaca tataaagacc 1021 cagataatcc cgagtacttg gaattcctga agcagttaaa acacctggcc tatgagcagt 1081 tcaacttcac catggaggat ggcctggtga acaccatccc agcatccttc cacgacgggc 1141 tcctgctcta tatccaggca gtgacggaga ctctggcaca tgggggaact gttactgatg 1201 gggagaacat cactcagcgg atgtggaacc gaagctttca aggtgtgaca ggatacctga 1261 aaattgatag cagtggcgat cgggaaacag acttctccct ctgggatatg gatcccgaga 1321 atggtgcctt cagggttgta ctgaactaca atgggacttc ccaagagctg gtggctgtgt 1381 cggggcgcaa actgaactgg cccctggggt accctcctcc tgacatcccc aaatgtggct 1441 ttgacaacga agacccagca tgcaaccaag atcacctttc caccctggag gtgctggctt 1501 tggtgggcag cctctccttg ctcggcattc tgattgtctc cttcttcata tacaggaaga 1561 tgcagctgga gaaggaactg gcctcggagc tgtggcgggt gcgctgggag gacgttgagc 1621 ccagtagcct tgagaggcac ctgcggagtg caggcagccg gctgaccctg agcgggagag 1681 gctccaatta cggctccctg ctaaccacag agggccagtt ccaagtcttt gccaagacag 1741 catattataa gggcaacctc gtggctgtga aacgtgtgaa ccgtaaacgc attgagctga 1801 cacgaaaagt cctgtttgaa ctgaagcata tgcgggatgt gcagaatgaa cacctgacca 1861 ggtttgtggg agcctgcacc gaccccccca atatctgcat cctcacagag tactgtcccc 1921 gtgggagcct gcaggacatt ctggagaatg agagcatcac cctggactgg atgttccggt 1981 actcactcac caatgacatc gtcaagggca tgctgtttct acacaatggg gctatctgtt 2041 cccatgggaa cctcaagtca tccaactgcg tggtagatgg gcgctttgtg ctcaagatca 2101 ccgactatgg gctggagagc ttcagggacc tggacccaga gcaaggacac accgtttatg 2161 ccaaaaagct gtggacggcc cctgagctcc tgcgaatggc ttcaccccct gtgcggggct 2221 cccaggctgg tgacgtatac agctttggga tcatccttca ggagattgcc ctgaggagtg 2281 gggtcttcca cgtggaaggt ttggacctga gccccaaaga gatcatcgag cgggtgactc 2341 ggggtgagca gccccccttc cggccctccc tggccctgca gagtcacctg gaggagttgg 2401 ggctgctcat gcagcggtgc tgggctgagg acccacagga gaggccacca ttccagcaga 2461 tccgcctgac gttgcgcaaa tttaacaggg agaacagcag caacatcctg gacaacctgc 2521 tgtcccgcat ggagcagtac gcgaacaatc tggaggaact ggtggaggag cggacccagg 2581 catacctgga ggagaagcgc aaggctgagg ccctgctcta ccagatcctg cctcactcag 2641 tggctgagca gctgaagcgt ggggagacgg tgcaggccga agcctttgac agtgttacca 2701 tctacttcag tgacattgtg ggtttcacag cgctgtcggc ggagagcacg cccatgcagg 2761 tggtgaccct gctcaatgac ctgtacactt gctttgatgc tgtcatagac aactttgatg 2821 tgtacaaggt ggagacaatt ggcgatgcct acatggtggt gtcagggctc cctgtgcgga 2881 acgggcggct acacgcctgc gaggtagccc gcatggccct ggcactgctg gatgctgtgc 2941 gctccttccg aatccgccac cggccccagg agcagctgcg cttgcgcatt ggcatccaca 3001 caggacctgt gtgtgctGGA GTGGTGGGAC TGAAGATGCC CCGTTACTGT CTCTTTGGGG 3061 ATACAGTCAA CACAGCCTCA AGAATGGAGT CTAATGGGGA AGCCCTGAAG ATCCACTTGT 3121 CTTCTGAGAC CAAGGCTGTC CTGGAGGAGT TTGGTGGTTT CGAGCTGGAG CTTCGAGGGG 3181 ATGTAGAAAT GAAGGGCAAA GGCAAGGTTC GGACCTACTG GCTCCTTGGG GAGAGGGGGA 3241 GTAGCACCCG AGGCTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3301 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3361 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTAACAC TCCCCGGCGC 3421 AGTGGAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt