Construct: ORF TRCN0000487854

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020264.1_s317c1
DNA Barcode:
GAATGTCTAGGATGTCATATAACA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
STRADA (92335)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487854
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 92335 STRADA STE20 related adaptor alpha NM_001363787.1 99.9% 99.7% 1079C>T
2 human 92335 STRADA STE20 related adaptor alpha XM_011525467.3 98.1% 97.5% (many diffs)
3 human 92335 STRADA STE20 related adaptor alpha NM_001003786.3 97.9% 97.7% 12_13ins24;1055C>T
4 human 92335 STRADA STE20 related adaptor alpha NM_001363786.1 94.5% 93.6% (many diffs)
5 human 92335 STRADA STE20 related adaptor alpha NM_001003787.4 93.1% 93% 37_123del;1166C>T
6 human 92335 STRADA STE20 related adaptor alpha NM_001003788.3 92.7% 92.5% 0_1ins87;992C>T
7 human 92335 STRADA STE20 related adaptor alpha XM_005257799.3 92.7% 92.5% 0_1ins87;992C>T
8 human 92335 STRADA STE20 related adaptor alpha XM_011525466.3 86.6% 80.8% (many diffs)
9 human 92335 STRADA STE20 related adaptor alpha NM_153335.6 85.4% 79.8% (many diffs)
10 human 92335 STRADA STE20 related adaptor alpha NM_001165969.2 78.1% 76.8% 921_922ins91;942_943ins173
11 human 92335 STRADA STE20 related adaptor alpha NM_001363789.1 76.1% 74.8% 12_13ins24;897_898ins91;918_919ins173
12 human 92335 STRADA STE20 related adaptor alpha NM_001363790.1 74.5% 68.2% (many diffs)
13 human 92335 STRADA STE20 related adaptor alpha NM_001363788.1 72.8% 71.6% 37_123del;1008_1009ins91;1029_1030ins173
14 human 92335 STRADA STE20 related adaptor alpha NM_001165970.2 72.5% 69.6% (many diffs)
15 human 92335 STRADA STE20 related adaptor alpha NM_001363791.1 70.8% 69.6% 0_1ins87;834_835ins91;855_856ins173
16 human 92335 STRADA STE20 related adaptor alpha XM_017025314.2 65.1% 64.1% (many diffs)
17 human 92335 STRADA STE20 related adaptor alpha XM_005257801.5 60.7% 59.8% (many diffs)
18 human 92335 STRADA STE20 related adaptor alpha NR_156741.2 47.6% (many diffs)
19 human 92335 STRADA STE20 related adaptor alpha XM_005257803.5 47.1% 39.1% (many diffs)
20 human 92335 STRADA STE20 related adaptor alpha XR_243688.3 42.7% (many diffs)
21 mouse 72149 Strada STE20-related kinase adapto... XM_006534294.3 87.8% 94.5% (many diffs)
22 mouse 72149 Strada STE20-related kinase adapto... NM_028126.3 86.1% 92.5% (many diffs)
23 mouse 72149 Strada STE20-related kinase adapto... XM_006534295.1 86.1% 92.5% (many diffs)
24 mouse 72149 Strada STE20-related kinase adapto... NM_001252449.1 83.4% 89.5% (many diffs)
25 mouse 72149 Strada STE20-related kinase adapto... XM_006534293.3 83.3% 88.6% (many diffs)
26 mouse 72149 Strada STE20-related kinase adapto... NM_001252448.1 81.9% 88.1% (many diffs)
27 mouse 72149 Strada STE20-related kinase adapto... XM_011249270.1 81.9% 88.1% (many diffs)
28 mouse 72149 Strada STE20-related kinase adapto... XM_006534296.3 81.7% 88.3% (many diffs)
29 mouse 72149 Strada STE20-related kinase adapto... XM_006534297.3 81.7% 88.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1278
ORF length:
1206
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtcattt cttgtaagta aaccagagcg aatcaggacc aatgatgcga
121gctcagagtc aatagcatcc ttctctaaac aggaggtcat gagtagcttt ctgccagagg
181gagggtgtta cgagctgctc actgtgatag gcaaaggatt tgaggacctg atgactgtga
241atctagcaag gtacaaacca acaggagagt acgtgactgt acggaggatt aacctagaag
301cttgttccaa tgagatggta acattcttgc agggcgagct gcatgtctcc aaactcttca
361accatcccaa tatcgtgcca tatcgagcca cttttattgc agacaatgag ctgtgggttg
421tcacatcatt catggcatac ggttctgcaa aagatctcat ctgtacacac ttcatggatg
481gcatgaatga gctggcgatt gcttacatcc tgcagggggt gctgaaggcc ctcgactaca
541tccaccacat gggatatgta cacaggagtg tcaaagccag ccacatcctg atctctgtgg
601atgggaaggt ctacctgtct ggtttgcgca gcaacctcag catgataagc catgggcagc
661ggcagcgagt ggtccacgat tttcccaagt acagtgtcaa ggttctgccg tggctcagcc
721ccgaggtcct ccagcagaat ctccagggtt atgatgccaa gtctgacatc tacagtgtgg
781gaatcacagc ctgtgaactg gccaacggcc atgtcccctt taaggatatg cctgccaccc
841agatgctgct agagaaactg aacggcacag tgccctgcct gttggatacc agcaccatcc
901ccgctgagga gctgaccatg agcccttcgc gctcagtggc caactctggc ctgagtgaca
961gcctgaccac cagcaccccc cggccctcca acggtgactc gccctcccac ccctaccacc
1021gaaCCTTCTC CCCCCACTTC CACCACTTTG TGGAGCAGTG CCTTCAGCGC AACCCGGATG
1081CCAGGCCCAG TGCCAGCACC CTCCTGAACC ACTCTTTCTT CAAGCAGATC AAGCGACGTG
1141CCTCAGAGGT TTTGCCCGAA TTGCTTCGTC CTGTCACCCC CATCACCAAT TTTGAGGGCA
1201GCCAGTCTCA GGACCACAGT GGAATCTTTG GCCTGGTAAC AAACCTGGAA GAGCTGGAGG
1261TGGACGATTG GGAGTTCTGA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1321CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1381AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGAAT GTCTAGGATG
1441TCATATAACA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)