Construct: ORF TRCN0000487867
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020191.1_s317c1
- DNA Barcode:
- TATTGTCCAGCGGTTTTTGCCCCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RPS6KA2 (6196)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487867
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_021135.6 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | XM_006715549.3 | 96.1% | 96.1% | (many diffs) |
| 3 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_001006932.3 | 95.6% | 92.7% | (many diffs) |
| 4 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_001318936.2 | 94.7% | 90.4% | (many diffs) |
| 5 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_001318938.1 | 87.7% | 87.7% | (many diffs) |
| 6 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_001318937.2 | 85.9% | 85.2% | (many diffs) |
| 7 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | NM_011299.4 | 87.6% | 95.4% | (many diffs) |
| 8 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | XM_006523199.3 | 84.4% | 91.8% | (many diffs) |
| 9 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | XM_006523198.3 | 84.4% | 92% | (many diffs) |
| 10 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | XM_006523200.1 | 74.7% | 81.7% | (many diffs) |
| 11 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | XM_006523201.1 | 74.7% | 81.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2271
- ORF length:
- 2199
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggacctg agcatgaaga agttcgccgt gcgcaggttc ttctctgtgt 121 acctgcgcag gaagtcgcgc tccaagagct ccagcctgag ccggctcgag gaagaaggtg 181 tcgtgaagga gatagacatc agccatcatg tgaaggaggg ctttgagaag gcagatcctt 241 cccagtttga gctgctgaag gttttaggac aaggatccta tggaaaggtg ttcctggtga 301 ggaaggtgaa ggggtccgac gctgggcagc tctacgccat gaaggtcctt aagaaagcca 361 ccctaaaagt tcgggaccga gtgagatcga agatggagag agacatcttg gcagaagtga 421 atcacccctt cattgtgaag cttcattatg cctttcagac ggaaggaaag ctctacctga 481 tcctggactt cctgcgggga ggggacctct tcacccggct ctccaaagag gtcatgttca 541 cggaggagga tgtcaagttc tacctggctg agctggcctt ggctttagac catctccaca 601 gcctggggat catctacaga gatctgaagc ctgagaacat cctcctggat gaagaggggc 661 acattaagat cacagatttc ggcctgagta aggaggccat tgaccacgac aagagagcgt 721 actccttctg cgggacgatc gagtacatgg cgcccgaggt ggtgaaccgg cgaggacaca 781 cgcagagtgc cgactggtgg tccttcggcg tgctcatgtt tgagatgctc acggggtccc 841 tgccgttcca ggggaaggac aggaaggaga ccatggctct catcctcaaa gccaagctgg 901 ggatgccgca gttcctcagt ggggaggcac agagtttgct gcgagctctc ttcaaacgga 961 acccctgcaa ccggctgggt gctggcattg acggagtgga ggaaattaag cgccatccct 1021 tctttgtgac catagactgg aacacgctgt accggaagga gatcaagcca ccgttcaaac 1081 cagcagtggg caggcctgag gacaccttcc actttgaccc cgagttcaca gcgcggacgc 1141 ccacagactc tcctggcgtc cccccgagtg caaacgctca tcacctgttt agaggattca 1201 gctttgtggc ctcaagcctg atccaggagc cctcacagca agatctgcac aaagtcccag 1261 ttcacccaat cgtgcagcag ttacacggga acaacatcca cttcaccgat ggctacgaga 1321 tcaaggagga catcggggtg ggctcctact cagtgtgcaa gcgatgtgtg cataaagcca 1381 cagacaccga gtatgccgtg aagatcattg ataagagcaa gagagacccc tcggaagaga 1441 ttgagatcct cctgcggtac ggccagcacc cgaacatcat caccctcaag gatgtctatg 1501 atgatggcaa gtttgtgtac ctggtaatgg agctgatgcg tggtggggag ctcctggacc 1561 gcatcctccg gcagagatac ttctcgaagc gcgaagccag tgacgtcctg tgcaccatca 1621 ccaagaccat ggactacctc cattcccagg gggttgttca tcgagacctg aagccgagta 1681 acatcctgta cagggatgag tcggggagcc cagaatccat ccgagtctgc gacttcggct 1741 ttgccaagca gctgcgcgcg gggaacgggc tgctcatgac accctgctac acggccaatt 1801 tcgtggcccc ggaggtcctg aagcgtcaag gctatgatgc ggcgtgtgac atctggagtt 1861 tggggatcct gttgtacacc atgctggcag gatttacccc ttttgcaaat gggccagacg 1921 atacccctga ggagattctg gcgcggatcg gcagtgggaa gtatgccctt tctgggggaa 1981 actgggactc gatatctgac gcagctaaag acgtcgtgtc caagatgctc cacgtggacc 2041 ctcatcagcg cctgacggcg atgcaagtgc tcaaacaccc gtgggtgGTC AACAGAGAGT 2101 ACCTGTCCCC AAACCAGCTC AGCCGACAGG ACGTGCACCT GGTGAAGGGC GCGATGGCCG 2161 CCACCTACTT TGCTCTAAAC AGAACACCTC AGGCCCCGCG GCTGGAGCCC GTGCTGTCGT 2221 CCAACCTGGC TCAGCGCAGA GGCATGAAGA GACTCACGTC CACGCGGTTG TGAGACCCAG 2281 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2341 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2401 TATCTTGTGG AAAGGACGAT ATTGTCCAGC GGTTTTTGCC CCTACGCGTT AAGTCgacaa 2461 tcaacctctg gattacaaaa tttgtgaaag att