Construct: ORF TRCN0000487882
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019266.1_s317c1
- DNA Barcode:
- ATGTCATAAATAATTCATTCATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CERK (64781)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487882
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64781 | CERK | ceramide kinase | NM_022766.6 | 99.9% | 100% | 1164T>C |
| 2 | human | 64781 | CERK | ceramide kinase | XM_017028909.1 | 92.3% | 91.6% | (many diffs) |
| 3 | mouse | 223753 | Cerk | ceramide kinase | XM_006520831.3 | 51.2% | 53.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1686
- ORF length:
- 1611
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgggg gcgacggggg cggcggagcc gctgcaatcc gtgctgtggg 121 tgaagcagca gcgctgcgcc gtgagcctgg agcccgcgcg ggctctgctg cgctggtggc 181 ggagcccggg gcccggagcc ggcgcccccg gcgcggatgc ctgctctgtg cctgtatctg 241 agatcatcgc cgttgaggaa acagacgttc acgggaaaca tcaaggcagt ggaaaatggc 301 agaaaatgga aaagccttac gcttttacag ttcactgtgt aaagagagca cgacggcacc 361 gctggaagtg ggcgcaggtg actttctggt gtccagagga gcagctgtgt cacttgtggc 421 tgcagaccct gcgggagatg ctggagaagc tgacgtccag accaaagcat ttactggtat 481 ttatcaaccc gtttggagga aaaggacaag gcaagcggat atatgaaaga aaagtggcac 541 cactgttcac cttagcctcc atcaccactg acatcatcgt tactgaacat gctaatcagg 601 ccaaggagac tctgtatgag attaacatag acaaatacga cggcatcgtc tgtgtcggcg 661 gagatggtat gttcagcgag gtgctgcacg gtctgattgg gaggacgcag aggagcgccg 721 gggtcgacca gaaccacccc cgggctgtgc tggtccccag tagcctccgg attggaatca 781 ttcccgcagg gtcaacggac tgcgtgtgtt actccaccgt gggcaccagc gacgcagaaa 841 cctcggcgct gcatatcgtt gttggggact cgctggccat ggatgtgtcc tcagtccacc 901 acaacagcac actccttcgc tactccgtgt ccctgctggg ctacggcttc tacggggaca 961 tcatcaagga cagtgagaag aaacggtggt tgggtcttgc cagatacgac ttttcaggtt 1021 taaagacctt cctctcccac cactgctatg aagggacagt gtccttcctc cctgcacaac 1081 acacggtggg atctccaagg gataggaagc cctgccgggc aggatgcttt gtttgcaggc 1141 aaagcaagca gcagctggag gaggagcaga agaaagcact gtatggtttg gaagctgcgg 1201 aggacgtgga ggagtggcaa gtcgtctgtg ggaagttcct ggccatcaat gccacaaaca 1261 tgtcctgtgc ttgtcgccgg agccccaggg gcctctcccc ggctgcccac ttgggagacg 1321 ggtcttctga cctcatcctc atccggaaat gctccaggtt caatttTCTG AGATTTCTCA 1381 TCAGGCACAC CAACCAGCAG GACCAGTTTG ACTTCACTTT TGTTGAAGTT TATCGCGTCA 1441 AGAAATTCCA GTTTACGTCG AAGCACATGG AGGATGAGGA CAGCGACCTC AAGGAGGGGG 1501 GGAAGAAGCG CTTTGGGCAC ATTTGCAGCA GCCACCCCTC CTGCTGCTGC ACCGTCTCCA 1561 ACAGCTCCTG GAACTGCGAC GGGGAGGTCC TGCACAGCCC TGCCATCGAG GTCAGAGTCC 1621 ACTGCCAGCT GGTTCGACTC TTTGCACGAG GAATTGAAGA GAATCCGAAG CCAGACTCAC 1681 ACAGCGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1741 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1801 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATGTCATAA ATAATTCATT CATCTACGCG 1861 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt