Transcript: Human NM_022766.6

Homo sapiens ceramide kinase (CERK), mRNA.

Source:
NCBI, updated 2019-08-27
Taxon:
Homo sapiens (human)
Gene:
CERK (64781)
Length:
4442
CDS:
105..1718

Additional Resources:

NCBI RefSeq record:
NM_022766.6
NBCI Gene record:
CERK (64781)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148273 ATGGAGGCTAAGGTGAACAG pXPR_003 TGG 471 29% 4 0.9155 CERK CERK 76330
2 BRDN0001146528 TTAACATAGACAAATACGAC pXPR_003 GGG 564 35% 5 0.2505 CERK CERK 76332
3 BRDN0001145454 CTGCGTGTGTTACTCCACCG pXPR_003 TGG 742 46% 7 0.2285 CERK CERK 76331
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_022766.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195338 CCGCTAGATAGGCTCTGATTT pLKO.1 4053 3UTR 100% 13.200 18.480 N CERK n/a
2 TRCN0000194889 CGGCTTAAACTTTGATCTGTA pLKO.1 2921 3UTR 100% 4.950 6.930 N CERK n/a
3 TRCN0000199644 GCGTCTCGGATGTCTGTCTTT pLKO.1 2703 3UTR 100% 4.950 6.930 N CERK n/a
4 TRCN0000199185 CGACGCGAAGTGCTTAACACA pLKO.1 2488 3UTR 100% 3.000 4.200 N CERK n/a
5 TRCN0000037688 GCCACGATGGATCGCTGGTTT pLKO.1 2733 3UTR 100% 1.650 2.310 N CERK n/a
6 TRCN0000289241 GCCACGATGGATCGCTGGTTT pLKO_005 2733 3UTR 100% 1.650 2.310 N CERK n/a
7 TRCN0000199723 GCCTGAAGTCTGTACACGTGG pLKO.1 2596 3UTR 100% 0.720 1.008 N CERK n/a
8 TRCN0000199620 GCTACGACAATCCGGCTGGGA pLKO.1 2353 3UTR 100% 0.000 0.000 N CERK n/a
9 TRCN0000037685 CACACTGAGTTCTCTATATTT pLKO.1 2642 3UTR 100% 15.000 10.500 N CERK n/a
10 TRCN0000289240 CACACTGAGTTCTCTATATTT pLKO_005 2642 3UTR 100% 15.000 10.500 N CERK n/a
11 TRCN0000195297 CCCTTAGTGATTTCATGTTTA pLKO.1 3018 3UTR 100% 13.200 9.240 N CERK n/a
12 TRCN0000296196 CCCTTAGTGATTTCATGTTTA pLKO_005 3018 3UTR 100% 13.200 9.240 N CERK n/a
13 TRCN0000195155 CCTGAATACAATCTTGAGTAA pLKO.1 4298 3UTR 100% 4.950 3.465 N CERK n/a
14 TRCN0000037684 CTGGCAAGTTTGTTACTGTTA pLKO.1 2441 3UTR 100% 4.950 3.465 N CERK n/a
15 TRCN0000289316 CTGGCAAGTTTGTTACTGTTA pLKO_005 2441 3UTR 100% 4.950 3.465 N CERK n/a
16 TRCN0000037686 TCCAGATTTGTCCTTGTCTTT pLKO.1 2617 3UTR 100% 4.950 3.465 N CERK n/a
17 TRCN0000037687 CACGGATGATAAGCTCTGGAA pLKO.1 2401 3UTR 100% 2.640 1.848 N CERK n/a
18 TRCN0000199448 GCTGGCAGTGTGTAAAGCACT pLKO.1 2422 3UTR 100% 2.640 1.848 N CERK n/a
19 TRCN0000310209 GCTGGCAGTGTGTAAAGCACT pLKO_005 2422 3UTR 100% 2.640 1.848 N CERK n/a
20 TRCN0000199885 GCGGCGAGGCTACGACAATCC pLKO.1 2345 3UTR 100% 0.000 0.000 N CERK n/a
21 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 3501 3UTR 100% 4.950 2.475 Y ERAP2 n/a
22 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3502 3UTR 100% 13.200 6.600 Y LIAS n/a
23 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 3668 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_022766.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487882 ATGTCATAAATAATTCATTCATCT pLX_317 20.1% 99.9% 100% V5 1164T>C n/a
2 TRCN0000488156 ATCAAAAGTCGAGTTTGTGCACTG pLX_317 20.3% 99.9% 100% V5 (not translated due to prior stop codon) 1164T>C n/a
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