Construct: ORF TRCN0000487892
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020431.1_s317c1
- DNA Barcode:
- ACCACTCCCTTCGGGGGACCGTGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NTRK1 (4914)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487892
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4914 | NTRK1 | neurotrophic receptor tyros... | NM_001012331.1 | 96.7% | 96.6% | 0_1ins78;787G>T;1656G>A |
| 2 | human | 4914 | NTRK1 | neurotrophic receptor tyros... | NM_002529.3 | 96% | 95.9% | (many diffs) |
| 3 | human | 4914 | NTRK1 | neurotrophic receptor tyros... | NM_001007792.1 | 90.9% | 86.4% | (many diffs) |
| 4 | mouse | 18211 | Ntrk1 | neurotrophic tyrosine kinas... | XM_006501124.2 | 82% | 83.5% | (many diffs) |
| 5 | mouse | 18211 | Ntrk1 | neurotrophic tyrosine kinas... | NM_001033124.1 | 81.4% | 82.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 2532
- ORF length:
- 2448
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatgtcgg gtgaggcctg gcagctgcag ctgggagcgc 121 acagacggct gccccgcctg agcgaggcgg gcgccgccgc gatgctgcga ggcggacggc 181 gcgggcagct tggctggcac agctgggctg cggggccggg cagcctgctg gcttggctga 241 tactggcatc tgcgggcgcc gcaccctgcc ccgatgcctg ctgcccccac ggctcctcgg 301 gactgcgatg cacccgggat ggggccctgg atagcctcca ccacctgccc ggcgcagaga 361 acctgactga gctctacatc gagaaccagc agcatctgca gcatctggag ctccgtgatc 421 tgaggggcct gggggagctg agaaacctca ccatcgtgaa gagtggtctc cgtttcgtgg 481 cgccagatgc cttccatttc actcctcggc tcagtcgcct gaatctctcc ttcaacgctc 541 tggagtctct ctcctggaaa actgtgcagg gcctctcctt acaggaactg gtcctgtcgg 601 ggaaccctct gcactgttct tgtgccctgc gctggctaca gcgctgggag gaggagggac 661 tgggcggagt gcctgaacag aagctgcagt gtcatgggca agggcccctg gcccacatgc 721 ccaatgccag ctgtggtgtg cccacgctga aggtccaggt gcccaatgcc tcggtggatg 781 tgggggacga cgtgctgctg cggtgccagg tggaggggcg gggcctggag caggccggct 841 ggatcctcac agagctggag cagtcagcca cggtgatgaa atctgggggt ctgccatccc 901 tggggctgac cctggccaat gtcaccagtg acctcaacag gaagaacttg acgtgctggg 961 cagagaacga tgtgggccgg gcagaggtct ctgttcaggt caacgtctcc ttcccggcca 1021 gtgtgcagct gcacacggcg gtggagatgc accactggtg catccccttc tctgtggatg 1081 ggcagccggc accgtctctg cgctggctct tcaatggctc cgtgctcaat gagaccagct 1141 tcatcttcac tgagttcctg gagccggcag ccaatgagac cgtgcggcac gggtgtctgc 1201 gcctcaacca gcccacccac gtcaacaacg gcaactacac gctgctggct gccaacccct 1261 tcggccaggc ctccgcctcc atcatggctg ccttcatgga caaccctttc gagttcaacc 1321 ccgaggaccc catccctgac actaacagca catctggaga cccggtggag aagaaggacg 1381 aaacaccttt tggggtctcg gtggctgtgg gcctggccgt ctttgcctgc ctcttccttt 1441 ctacgctgct ccttgtgctc aacaaatgtg gacggagaaa caagtttggg atcaaccgcc 1501 cggctgtgct ggctccagag gatgggctgg ccatgtccct gcatttcatg acattgggtg 1561 gcagctccct gtcccccacc gagggcaaag gctctgggct ccaaggccac atcatcgaga 1621 acccacaata cttcagtgat gcctgtgttc accacatcaa gcgccgggac atcgtgctca 1681 agtgggagct gggggagggc gcctttggga aggtcttcct tgctgagtgc cacaacctcc 1741 tgcctgagca ggacaagatg ctggtggctg tcaaggcact gaaggaggcg tccgagagtg 1801 ctcggcagga cttccaacgt gaggctgagc tgctcaccat gctgcagcac cagcacatcg 1861 tgcgcttctt cggcgtctgc accgagggcc gccccctgct catggtcttt gagtatatgc 1921 ggcacgggga cctcaaccgc ttcctccgat cccatggacc tgatgccaag ctgctggctg 1981 gtggggagga tgtggctcca ggccccctgg gtctggggca gctgctggcc gtggctagcc 2041 aggtcgctgc ggggatggtg tacctggcgg gtctgcattt tgtgcaccgg gacctggcca 2101 cacgcaactg tctagtgggc cagggactgg tggtcaagat tggtgatttt ggcatgagca 2161 gggatatcta cagcaccgac tattaccgtg tgggaggccg caccatgctg cccattcgct 2221 ggatgccgcc cgagagcatc ctgtaccgta agttcaccac cgagagcgac gtgtggagct 2281 tcggcgtggt gctctgggag atcttcaccT ACGGCAAGCA GCCCTGGTAC CAGCTCTCCA 2341 ACACGGAGGC AATCGACTGC ATCACGCAGG GACGTGAGTT GGAGCGGCCA CGTGCCTGCC 2401 CACCAGAGGT CTACGCCATC ATGCGGGGCT GCTGGCAGCG GGAGCCCCAG CAACGCCACA 2461 GCATCAAGGA TGTGCACGCC CGGCTGCAAG CCCTGGCCCA GGCACCTCCT GTCTACCTGG 2521 ATGTCCTGGG CTAGAAGCTT AAGGGTGGGC GCGCCGACCC AGCTTTCTTG TACAAAGTGG 2581 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2641 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2701 AACCACTCCC TTCGGGGGAC CGTGAACGCG TTAAGTCgac aatcaacctc tggattacaa 2761 aatttgtgaa agatt