Construct: ORF TRCN0000487936
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020710.1_s317c1
- DNA Barcode:
- ACAGCAAACCTACTCCTACTAAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TLK1 (9874)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487936
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9874 | TLK1 | tousled like kinase 1 | NM_012290.5 | 100% | 100% | |
| 2 | human | 9874 | TLK1 | tousled like kinase 1 | NM_001136554.2 | 93.7% | 93.7% | 0_1ins144 |
| 3 | human | 9874 | TLK1 | tousled like kinase 1 | XM_011512238.3 | 91.7% | 91.7% | 980_981ins189 |
| 4 | human | 9874 | TLK1 | tousled like kinase 1 | NM_001136555.2 | 86.6% | 85.6% | (many diffs) |
| 5 | human | 9874 | TLK1 | tousled like kinase 1 | XM_017005422.2 | 71.6% | 71.6% | 0_1ins651 |
| 6 | human | 9874 | TLK1 | tousled like kinase 1 | XM_024453261.1 | 71.6% | 71.6% | 0_1ins651 |
| 7 | human | 9874 | TLK1 | tousled like kinase 1 | XM_024453262.1 | 71.6% | 71.6% | 0_1ins651 |
| 8 | human | 9874 | TLK1 | tousled like kinase 1 | XR_001739084.2 | 46.9% | 1_387del;1622_1747del;1834_1835ins977 | |
| 9 | human | 9874 | TLK1 | tousled like kinase 1 | XR_001739085.2 | 46.2% | 1_387del;1622_1879del;2944_4972del | |
| 10 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | NM_172664.3 | 91.9% | 98.1% | (many diffs) |
| 11 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_006499189.3 | 87.5% | 93.4% | (many diffs) |
| 12 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_006499188.3 | 87.3% | 92.6% | (many diffs) |
| 13 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_006499190.3 | 85.6% | 92% | (many diffs) |
| 14 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_006499191.1 | 65.4% | 70.4% | (many diffs) |
| 15 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_006499193.1 | 65.4% | 70.4% | (many diffs) |
| 16 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_017317580.1 | 65.4% | 70.4% | (many diffs) |
| 17 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_017317581.1 | 65.4% | 70.4% | (many diffs) |
| 18 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XR_374441.3 | 45.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2370
- ORF length:
- 2298
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagtgtc caaagtagca gtggaagttt ggaggggccg ccatcttggt 121 cccagctctc cacgtctcca accccgggct cggcggcggc ggccaggtcc ctgctgaatc 181 acacgccgcc atccgggagg cccagggaag gtgcaatgga tgagcttcat agtctggatc 241 caagaaggca agagttattg gaagctagat ttactggagt tgcaagtggg agcactggaa 301 gtacgggcag ttgcagtgtt ggagctaaag cctcaacaaa taacgaaagc tctaatcaca 361 gttttggaag cttgggatct ttaagtgaca aagaatcaga gacaccggag aagaaacaat 421 cggaatcatc caggggaaga aagagaaaag cagaaaacca gaatgaaagt agtcagggaa 481 aaagtattgg gggacgtggc cacaaaatta gcgactattt tgaataccag ggtggaaatg 541 gctcaagtcc agtaagaggc atacctcctg caatccgttc tcctcaaaat tcacattcac 601 attccactcc ttcctcatct gttcgaccga atagcccttc tcctactgca ttagcatttg 661 gggaccaccc tattgtacaa ccaaagcaat tatcctttaa aattattcag actgatctca 721 caatgctgaa attagcagca ttagaaagta ataaaatcca ggacctggaa aagaaggagg 781 gacgtataga tgatttgctc agggctaact gtgatctcag acggcaaata gatgaacaac 841 aaaaattact tgaaaaatac aaagaacgat taaataagtg catatcaatg agcaagaaac 901 ttcttattga aaagagtaca caagaaaagc tgtcaagcag agagaagagt atgcaagatc 961 gattacgcct cgggcacttt acaacagtta gacatggcgc ttcatttact gaacaatgga 1021 cagatggttt tgcatttcag aatcttgtga agcaacaaga atgggtgaat cagcaaaggg 1081 aagatattga aaggcaaagg aaacttctag ccaaacgcaa acctcccaca gctaataatt 1141 ctcaggcacc ctctaccaat tctgaaccaa aacaaaggaa aaacaaagca gtcaatggag 1201 cagagaatga tccctttgtt agaccaaatt taccacaact gttgactttg gcagaatatc 1261 atgaacagga agaaattttc aaacttagac taggacatct caaaaaggaa gaggcagaaa 1321 tccaggcaga acttgaacgt ttggaaagag tcagaaatct tcacatacgt gagctgaaaa 1381 gaataaacaa tgaagataat tcacagttca aagatcaccc aacattaaat gaaagatatt 1441 tattacttca tctgcttggt agaggtggct ttagtgaagt gtataaggct tttgaccttt 1501 atgaacaaag atatgctgct gtgaagatac atcagcttaa taaaagctgg agagatgaga 1561 agaaagaaaa ctaccacaaa catgcctgca gagagtatag aatacacaaa gaactggatc 1621 accccagaat agttaaactc tatgattatt tctccttgga tacagatacg ttttgtacag 1681 tgttagaata ctgtgaaggc aatgacttgg atttctatct gaagcaacac aagttaatgt 1741 cagagaaaga agctcggtct attgtaatgc agattgtaaa tgcactaaga tatctcaatg 1801 agatcaaacc ccctattata cattatgatc ttaagccagg aaacatccta ctggtagatg 1861 gaacagcatg tggtgaaatc aaaatcactg attttggtct gtccaagatt atggatgatg 1921 atagctatgg tgtagatgga atggatctaa cttcccaggg ggcaggcact tactggtatt 1981 tacctcctga gtgttttgta gttggaaaag agccaccaaa gatttccaac aaggttgatg 2041 tatggtcggt tggagtcatc ttctttcagt gtctttatgG TAGAAAGCCA TTTGGTCACA 2101 ATCAATCTCA ACAAGACATT CTTCAAGAAA ATACAATATT AAAAGCCACA GAAGTCCAGT 2161 TCCCTGTAAA ACCGGTTGTA AGCAGTGAAG CCAAGGCATT TATAAGACGC TGTTTGGCAT 2221 ATCGAAAAGA AGATCGATTT GATGTGCACC AGCTGGCAAA TGACCCATAC CTTCTCCCAC 2281 ACATGAGAAG ATCAAATTCT TCAGGAAACC TACACATGGC TGGGCTGACA GCATCCCCTA 2341 CACCCCCTTC TTCAAGCATA ATTACTTACT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 2401 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2461 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 2521 AGCAAACCTA CTCCTACTAA ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 2581 ttgtgaaaga tt