Construct: ORF TRCN0000487954
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019549.1_s317c1
- DNA Barcode:
- AGCGCTCGGAAATCGTTCTGTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRM8 (2918)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487954
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001127323.1 | 99.8% | 99.6% | 235A>G;1085T>A;2724_2725insG |
| 2 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371085.1 | 99.8% | 99.6% | 235A>G;1085T>A;2724_2725insG |
| 3 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_000845.3 | 98.4% | 98.2% | (many diffs) |
| 4 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371083.1 | 98.4% | 98.2% | (many diffs) |
| 5 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371084.1 | 98.4% | 98.2% | (many diffs) |
| 6 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371086.1 | 96.4% | 95% | (many diffs) |
| 7 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516094.1 | 81.8% | 81.1% | (many diffs) |
| 8 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516095.2 | 78.5% | 78.3% | 1_1delAins583;503T>A;2142_2143insG |
| 9 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516101.2 | 77.3% | 77.2% | 0_1ins615;470T>A;2109_2110insG |
| 10 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516102.2 | 77.3% | 77.2% | 0_1ins615;470T>A;2109_2110insG |
| 11 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371087.1 | 76% | 75.7% | (many diffs) |
| 12 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012075.1 | 76% | 75.7% | (many diffs) |
| 13 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012076.1 | 76% | 75.7% | (many diffs) |
| 14 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012077.1 | 76% | 75.7% | (many diffs) |
| 15 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012078.1 | 76% | 75.7% | (many diffs) |
| 16 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012079.1 | 76% | 75.7% | (many diffs) |
| 17 | human | 2918 | GRM8 | glutamate metabotropic rece... | NR_028041.1 | 74.3% | (many diffs) | |
| 18 | human | 2918 | GRM8 | glutamate metabotropic rece... | NR_163850.1 | 69.7% | (many diffs) | |
| 19 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516103.2 | 69.2% | 69% | 0_1ins837;248T>A;1887_1888insG |
| 20 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012080.1 | 69.2% | 69% | 0_1ins837;248T>A;1887_1888insG |
| 21 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_024446736.1 | 69.2% | 69% | 0_1ins837;248T>A;1887_1888insG |
| 22 | human | 2918 | GRM8 | glutamate metabotropic rece... | NR_163849.1 | 68.4% | (many diffs) | |
| 23 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516108.2 | 59.8% | 58.1% | (many diffs) |
| 24 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371088.1 | 50% | 49.5% | (many diffs) |
| 25 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | NM_008174.2 | 91.8% | 97.9% | (many diffs) |
| 26 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_006504987.3 | 91.8% | 97.9% | (many diffs) |
| 27 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_006504989.1 | 91.8% | 97.9% | (many diffs) |
| 28 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_006504990.3 | 91.8% | 97.9% | (many diffs) |
| 29 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_006504991.3 | 91.8% | 97.9% | (many diffs) |
| 30 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | NM_001311072.1 | 90.5% | 96.6% | (many diffs) |
| 31 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_017321397.1 | 90.5% | 96.6% | (many diffs) |
| 32 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_017321398.1 | 63.7% | 67.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2802
- ORF length:
- 2727
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggta tgcgagggaa agcgatcagc ctcttgccct tgtttcttcc 121 tcttgaccgc caagttctac tggatcctca caatgatgca aagaactcac agccaggagt 181 atgcccattc catacgggtg gatggggaca ttattttggg gggtctcttc cctgtccacg 241 caaagggaga gagaggggtg ccttgtgggg agctgaagaa ggaaaagggg attcacagac 301 tggaggccgt gctttatgca attgaccaga ttaacaagga ccctgatctc ctttccaaca 361 tcactctggg tgtccgcatc ctcgacacgt gctctaggga cacctatgct ttggagcagt 421 ctctaacatt cgtgcaggca ttaatagaga aagatgcttc ggatgtgaag tgtgctaatg 481 gagatccacc cattttcacc aagcccgaca agatttctgg cgtcataggt gctgcagcaa 541 gctccgtgtc catcatggtt gctaacattt taagactttt taagatacct caaatcagct 601 atgcatccac agccccagag ctaagtgata acaccaggta tgactttttc tctcgagtgg 661 ttccgcctga ctcctaccaa gcccaagcca tggtggacat cgtgacagca ctgggatgga 721 attatgtttc gacactggct tctgagggga actatggtga gagcggtgtg gaggccttca 781 cccagatctc gagggagatt ggtggtgttt gcattgctca gtcacagaaa atcccacgtg 841 aaccaagacc tggagaattt gaaaaaatta tcaaacgcct gctagaaaca cctaatgctc 901 gagcagtgat tatgtttgcc aatgaggatg acatcaggag gatattggaa gcagcaaaaa 961 aactaaacca aagtgggcat tttctctgga ttggctcaga tagttgggga tccaaaatag 1021 cacctgtcta tcagcaagag gagattgcag aaggggctgt gacaattttg cccaaacgag 1081 catcaattga tggatttgat cgatacttta gaagccgaac tcttgccaat aatcgaagaa 1141 atgtgtggtt tgcagaatac tgggaggaga attttggctg caagttagga tcacatggga 1201 aaaggaacag tcatataaag aaatgcacag ggctggagcg aattgctcgg gattcatctt 1261 atgaacagga aggaaaggtc caatttgtaa ttgatgctgt atattccatg gcttacgccc 1321 tgcacaatat gcacaaagat ctctgccctg gatacattgg cctttgtcca cgaatgagta 1381 ccattgatgg gaaagagcta cttggttata ttcgggctgt aaattttaat ggcagtgctg 1441 gcactcctgt cacttttaat gaaaacggag atgctcctgg acgttatgat atcttccagt 1501 atcaaataac caacaaaagc acagagtaca aagtcatcgg ccactggacc aatcagcttc 1561 atctaaaagt ggaagacatg cagtgggctc atagagaaca tactcacccg gcgtctgtct 1621 gcagcctgcc gtgtaagcca ggggagagga agaaaacggt gaaaggggtc ccttgctgct 1681 ggcactgtga acgctgtgaa ggttacaact accaggtgga tgagctgtcc tgtgaacttt 1741 gccctctgga tcagagaccc aacatgaacc gcacaggctg ccagcttatc cccatcatca 1801 aattggagtg gcattctccc tgggctgtgg tgcctgtgtt tgttgcaata ttgggaatca 1861 tcgccaccac ctttgtgatc gtgacctttg tccgctataa tgacacacct atcgtgaggg 1921 cttcaggacg cgaacttagt tacgtgctcc taacggggat ttttctctgt tattcaatca 1981 cgtttttaat gattgcagca ccagatacaa tcatatgctc cttccgacgg gtcttcctag 2041 gacttggcat gtgtttcagc tatgcagccc ttctgaccaa aacaaaccgt atccaccgaa 2101 tatttgagca ggggaagaaa tctgtcacag cgcccaagtt cattagtcca gcatctcagc 2161 tggtgatcac cttcagcctc atctccgtcc agctccttgg agtgtttgtc tggtttgttg 2221 tggatccccc ccacatcatc attgactatg gagagcagcg gacactagat ccagagaagg 2281 ccaggggagt gctcaagtgt gacatttctg atctctcact catttgttca cttggataca 2341 gtatcctctt gatggtcact tgtactgttt atgccattaa aacgagaggt gtcccagaga 2401 ctttcaatga agccaaacct attggattta ccatgtatac cacctgcatc atttggttag 2461 ctttcatccC CATCTTTTTT GGTACAGCCC AGTCAGCAGA AAAGATGTAC ATCCAGACAA 2521 CAACACTTAC TGTCTCCATG AGTTTAAGTG CTTCAGTATC TCTGGGCATG CTCTATATGC 2581 CCAAGGTTTA TATTATAATT TTTCATCCAG AACAGAATGT TCAAAAACGC AAGAGGAGCT 2641 TCAAGGCTGT GGTGACAGCT GCCACCATGC AAAGCAAACT GATCCAAAAA GGAAATGACA 2701 GACCAAATGG CGAGGTGAAA AGTGAACTCT GTGAGAGTCT TGAAACCAAC AGTAAGTCAT 2761 CTGTAGAGTT TCCGATGGTC AAGAGCGGGA GCACTTCCGA CCCAGCTTTC TTGTACAAAG 2821 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2881 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2941 ACGAAGCGCT CGGAAATCGT TCTGTCGGAC GCGTTAAGTC gacaatcaac ctctggatta 3001 caaaatttgt gaaagatt