Construct: ORF TRCN0000487985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019067.1_s317c1
- DNA Barcode:
- AGCCCTCCCGCTAGCATAACCTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K2 (5605)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487985
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5605 | MAP2K2 | mitogen-activated protein k... | NM_030662.3 | 99.9% | 99.7% | 1200_1201insG |
| 2 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_006722799.2 | 76.6% | 76.5% | 705_706ins279;921_922insG |
| 3 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026989.1 | 75.4% | 67.6% | (many diffs) |
| 4 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026990.1 | 71.1% | 50% | (many diffs) |
| 5 | human | 407835 | LOC407835 | mitogen-activated protein k... | NR_002144.1 | 65.9% | (many diffs) | |
| 6 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026991.1 | 61.6% | 60% | (many diffs) |
| 7 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | NM_001347144.1 | 87.1% | 94% | (many diffs) |
| 8 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | NM_023138.5 | 86.9% | 93.7% | (many diffs) |
| 9 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | XM_006513684.3 | 45.2% | 48.1% | (many diffs) |
| 10 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | XM_006513683.3 | 45% | 48% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1275
- ORF length:
- 1203
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctggcc cggaggaagc cggtgctgcc ggcgctcacc atcaacccta 121 ccatcgccga gggcccatcc cctaccagcg agggcgcctc cgaggcaaac ctggtggacc 181 tgcagaagaa gctggaggag ctggaacttg acgagcagca gaagaagcgg ctggaagcct 241 ttctcaccca gaaagccaag gtcggcgaac tcaaagacga tgacttcgaa aggatctcag 301 agctgggcgc gggcaacggc ggggtggtca ccaaagtcca gcacagaccc tcgggcctca 361 tcatggccag gaagctgatc caccttgaga tcaagccggc catccggaac cagatcatcc 421 gcgagctgca ggtcctgcac gaatgcaact cgccgtacat cgtgggcttc tacggggcct 481 tctacagtga cggggagatc agcatttgca tggaacacat ggacggcggc tccctggacc 541 aggtgctgaa agaggccaag aggattcccg aggagatcct ggggaaagtc agcatcgcgg 601 ttctccgggg cttggcgtac ctccgagaga agcaccagat catgcaccga gatgtgaagc 661 cctccaacat cctcgtgaac tctagagggg agatcaagct gtgtgacttc ggggtgagcg 721 gccagctcat cgactccatg gccaactcct tcgtgggcac gcgctcctac atggctccgg 781 agcggttgca gggcacacat tactcggtgc agtcggacat ctggagcatg ggcctgtccc 841 tggtggagct ggccgtcgga aggtacccca tccccccgcc cgacgccaaa gagctggagg 901 ccatctttgg ccggcccgtg gtcgacgggg aagaaggaga gcctcacagc atctcgccTC 961 GGCCGAGGCC CCCCGGGCGC CCCGTCAGCG GTCACGGGAT GGATAGCCGG CCTGCCATGG 1021 CCATCTTTGA ACTCCTGGAC TATATTGTGA ACGAGCCACC TCCTAAGCTG CCCAACGGTG 1081 TGTTCACCCC CGACTTCCAG GAGTTTGTCA ATAAATGCCT CATCAAGAAC CCAGCGGAGC 1141 GGGCGGACCT GAAGATGCTC ACAAACCACA CCTTCATCAA GCGGTCCGAG GTGGAAGAAG 1201 TGGATTTTGC CGGCTGGTTG TGTAAAACCC TGCGGCTGAA CCAGCCCGGC ACACCCACGC 1261 GCACCGCCGT GGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1321 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1381 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGC CCTCCCGCTA GCATAACCTA 1441 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t