Construct: ORF TRCN0000487986
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020100.1_s317c1
- DNA Barcode:
- TATAGGCATGGAACCCGGCATCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK32C (282974)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487986
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 282974 | STK32C | serine/threonine kinase 32C | NM_173575.4 | 99.7% | 100% | 1203T>C;1452G>T;1458C>T |
| 2 | human | 282974 | STK32C | serine/threonine kinase 32C | XM_011539690.2 | 98.7% | 98.9% | (many diffs) |
| 3 | human | 282974 | STK32C | serine/threonine kinase 32C | NM_001318878.2 | 87.6% | 80.9% | (many diffs) |
| 4 | human | 282974 | STK32C | serine/threonine kinase 32C | XM_011539688.1 | 86.8% | 80.1% | (many diffs) |
| 5 | human | 282974 | STK32C | serine/threonine kinase 32C | NM_001318879.1 | 75.7% | 75.9% | (many diffs) |
| 6 | human | 282974 | STK32C | serine/threonine kinase 32C | XM_011539693.2 | 75.7% | 75.9% | (many diffs) |
| 7 | human | 282974 | STK32C | serine/threonine kinase 32C | XM_011539694.1 | 75.7% | 75.9% | (many diffs) |
| 8 | human | 282974 | STK32C | serine/threonine kinase 32C | XM_011539695.1 | 75.7% | 75.9% | (many diffs) |
| 9 | human | 282974 | STK32C | serine/threonine kinase 32C | XM_011539696.1 | 75.7% | 75.9% | (many diffs) |
| 10 | human | 282974 | STK32C | serine/threonine kinase 32C | XM_024447947.1 | 75.7% | 75.9% | (many diffs) |
| 11 | human | 282974 | STK32C | serine/threonine kinase 32C | XM_024447948.1 | 75.7% | 75.9% | (many diffs) |
| 12 | human | 282974 | STK32C | serine/threonine kinase 32C | XM_024447949.1 | 75.7% | 75.9% | (many diffs) |
| 13 | human | 282974 | STK32C | serine/threonine kinase 32C | XR_945687.2 | 70.5% | (many diffs) | |
| 14 | human | 282974 | STK32C | serine/threonine kinase 32C | NR_134911.1 | 60.6% | (many diffs) | |
| 15 | mouse | 57740 | Stk32c | serine/threonine kinase 32C | NM_021302.3 | 86.7% | 90.9% | (many diffs) |
| 16 | mouse | 57740 | Stk32c | serine/threonine kinase 32C | XM_006536216.3 | 78.3% | 75.3% | (many diffs) |
| 17 | mouse | 57740 | Stk32c | serine/threonine kinase 32C | NM_001162540.1 | 66% | 70% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 102
- ORF end:
- 1560
- ORF length:
- 1458
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccggat ccgacctggc gatgaggagt ggcgccgagc 121 gcaggggcag cagcgccgcg gcgtccccgg gctcgccgcc ccccggccgc gcgcgccccg 181 ccggctccga cgcgccctcg gccctgccgc cgcccgctgc tggccagccc cgggcccggg 241 actcgggcga tgtccgctcg cagccgcgcc ccctgtttca gtggagcaag tggaagaaga 301 ggatgggctc gtccatgtcg gcggccaccg cgcggaggcc ggtgtttgac gacaaggagg 361 acgtgaactt cgaccacttc cagatccttc gggccattgg gaagggcagc tttggcaagg 421 tgtgcattgt gcagaagcgg gacacggaga agatgtacgc catgaagtac atgaacaagc 481 agcagtgcat cgagcgcgac gaggtccgca acgtcttccg ggagctggag atcctgcagg 541 agatcgagca cgtcttcctg gtgaacctct ggtactcctt ccaggacgag gaggacatgt 601 tcatggtcgt ggacctgcta ctgggcgggg acctgcgcta ccacctgcag cagaacgtgc 661 agttctccga ggacacggtg aggctgtaca tctgcgagat ggcactggct ctggactacc 721 tgcgcggcca gcacatcatc cacagagatg tcaagcctga caacattctc ctggatgaga 781 gaggacatgc acacctgacc gacttcaaca ttgccaccat catcaaggac ggggagcggg 841 cgacggcatt agcaggcacc aagccgtaca tggctccgga gatcttccac tcttttgtca 901 acggcgggac cggctactcc ttcgaggtgg actggtggtc ggtgggggtg atggcctatg 961 agctgctgcg aggatggagg ccctatgaca tccactccag caacgccgtg gagtccctgg 1021 tgcagctgtt cagcaccgtg agcgtccagt atgtccccac gtggtccaag gagatggtgg 1081 ccttgctgcg gaagctcctc actgtgaacc ccgagcaccg gctctccagc ctccaggacg 1141 tgcaggcagc cccggcgctg gccggcgtgc tgtgggacca cctgagcgag aagagggtgg 1201 agccgggctt cgtgcccaac aaaggccgtc tgcactgcga ccccaccttt gagctggagg 1261 agatgatcct ggagtccagg cccctgcaca agaagaagaa gcgcctggCC AAGAACAAGT 1321 CCCGGGACAA CAGCAGGGAC AGCTCCCAGT CCGAGAATGA CTATCTTCAA GACTGCCTCG 1381 ATGCCATCCA GCAAGACTTC GTGATTTTTA ACAGAGAAAA GCTGAAGAGG AGCCAGGACC 1441 TCCCGAGGGA GCCTCTCCCC GCCCCTGAGT CCAGGGATGC TGCGGAGCCT GTGGAGGACG 1501 AGGCGGAACG CTCCGCCCTG CCCATGTGCG GCCCCATTTG CCCCTCGGCC GGTAGCGGTA 1561 AGGGTGGGCG CGCCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1621 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1681 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TATAGGCATG GAACCCGGCA 1741 TCTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt