Construct: ORF TRCN0000488000
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020875.1_s317c1
- DNA Barcode:
- GTTGGTCTACTGTAAAAATCCTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HTR2A (3356)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488000
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3356 | HTR2A | 5-hydroxytryptamine recepto... | NM_000621.4 | 100% | 100% | |
| 2 | human | 3356 | HTR2A | 5-hydroxytryptamine recepto... | NM_001165947.2 | 78.5% | 72.6% | (many diffs) |
| 3 | mouse | 15558 | Htr2a | 5-hydroxytryptamine (seroto... | NM_172812.2 | 87.5% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1485
- ORF length:
- 1413
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatatt ctttgtgaag aaaatacttc tttgagctca actacgaact 121 ccctaatgca attaaatgat gacaccaggc tctacagtaa tgactttaac tccggagaag 181 ctaacacttc tgatgcattt aactggacag tcgactctga aaatcgaacc aacctttcct 241 gtgaagggtg cctctcaccg tcgtgtctct ccttacttca tctccaggaa aaaaactggt 301 ctgctttact gacagccgta gtgattattc taactattgc tggaaacata ctcgtcatca 361 tggcagtgtc cctagagaaa aagctgcaga atgccaccaa ctatttcctg atgtcacttg 421 ccatagctga tatgctgctg ggtttccttg tcatgcccgt gtccatgtta accatcctgt 481 atgggtaccg gtggcctctg ccgagcaagc tttgtgcagt ctggatttac ctggacgtgc 541 tcttctccac ggcctccatc atgcacctct gcgccatctc gctggaccgc tacgtcgcca 601 tccagaatcc catccaccac agccgcttca actccagaac taaggcattt ctgaaaatca 661 ttgctgtttg gaccatatca gtaggtatat ccatgccaat accagtcttt gggctacagg 721 acgattcgaa ggtctttaag gaggggagtt gcttactcgc cgatgataac tttgtcctga 781 tcggctcttt tgtgtcattt ttcattccct taaccatcat ggtgatcacc tactttctaa 841 ctatcaagtc actccagaaa gaagctactt tgtgtgtaag tgatcttggc acacgggcca 901 aattagcttc tttcagcttc ctccctcaga gttctttgtc ttcagaaaag ctcttccagc 961 ggtcgatcca tagggagcca gggtcctaca caggcaggag gactatgcag tccatcagca 1021 atgagcaaaa ggcatgcaag gtgctgggca tcgtcttctt cctgtttgtg gtgatgtggt 1081 gccctttctt catcacaaac atcatggccg tcatctgcaa agagtcctgc aatgaggatg 1141 tcattGGGGC CCTGCTCAAT GTGTTTGTTT GGATCGGTTA TCTCTCTTCA GCAGTCAACC 1201 CACTAGTCTA CACACTGTTC AACAAGACCT ATAGGTCAGC CTTTTCACGG TATATTCAGT 1261 GTCAGTACAA GGAAAACAAA AAACCATTGC AGTTAATTTT AGTGAACACA ATACCGGCTT 1321 TGGCCTACAA GTCTAGCCAA CTTCAAATGG GACAAAAAAA GAATTCAAAG CAAGATGCCA 1381 AGACAACAGA TAATGACTGC TCAATGGTTG CTCTAGGAAA GCAGCATTCT GAAGAGGCTT 1441 CTAAAGACAA TAGCGACGGA GTGAATGAAA AGGTGAGCTG TGTGTAGGAC CCAGCTTTCT 1501 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1561 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1621 GTGGAAAGGA CGAGTTGGTC TACTGTAAAA ATCCTTCACG CGTTAAGTCg acaatcaacc 1681 tctggattac aaaatttgtg aaagatt