Construct: ORF TRCN0000488001
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020407.1_s317c1
- DNA Barcode:
- GGGCTCAGCGAAGGCACAAATGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STRADB (55437)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488001
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55437 | STRADB | STE20 related adaptor beta | NM_018571.6 | 99.9% | 100% | 756C>T |
| 2 | human | 55437 | STRADB | STE20 related adaptor beta | NM_001206864.2 | 89.6% | 88.9% | (many diffs) |
| 3 | human | 55437 | STRADB | STE20 related adaptor beta | XM_005246669.2 | 68.1% | 66.2% | 756C>T;823_824ins206;855_856ins193 |
| 4 | human | 55437 | STRADB | STE20 related adaptor beta | XM_017004438.1 | 66.9% | 66.9% | 0_1ins414;342C>T |
| 5 | mouse | 227154 | Stradb | STE20-related kinase adapto... | NM_172656.5 | 89.6% | 90.9% | (many diffs) |
| 6 | mouse | 227154 | Stradb | STE20-related kinase adapto... | XR_373251.2 | 40.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1326
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtctctt ttggattgct tctgcacttc aagaacacaa gttgaatcac 121 tcagacctga aaaacagtct gaaaccagta tccatcaata cttggttgat gagccaaccc 181 tttcctggtc acgtccatcc actagagcca gtgaagtact atgttccacc aacgtttctc 241 actatgagct ccaagtagaa ataggaagag gatttgacaa cttgacttct gtccatcttg 301 cacggcatac tcccacagga acactggtaa ctataaaaat tacaaatctg gaaaactgca 361 atgaagaacg cctgaaagct ttacagaaag ccgtgattct atcccacttt ttccggcatc 421 ccaatattac aacttattgg acagttttca ctgttggcag ctggctttgg gttatttctc 481 catttatggc ctatggttca gcaagtcaac tcttgaggac ctattttcct gaaggaatga 541 gtgaaacttt aataagaaac attctctttg gagccgtgag agggttgaac tatctgcacc 601 aaaatggctg tattcacagg agtattaaag ccagccatat cctcatttct ggtgatggcc 661 tagtgaccct ctctggcctg tcccatctgc atagtttggt taagcatgga cagaggcata 721 gggctgtgta tgatttccca cagttcagca catcagtgca gccgtggctg agtccagaac 781 tactgagaca ggatttacat gggtataatg tgaagtcaga tatttatagt gttgggatta 841 cagcatgtga attagccagt gggcaggtgc ctttccagga catgcataga actcagatgc 901 tgttacagaa actgaaaggt cctccttata gcccattgga tatcagtatt ttccctcaat 961 cagaatccag aatgaaaaat tcccagtcag gtgtagactc tgggattGGA GAAAGTGTGC 1021 TTGTCTCCAG TGGAACTCAC ACAGTAAATA GTGACCGATT ACACACACCA TCCTCAAAAA 1081 CTTTCTCTCC TGCCTTCTTT AGCTTGGTAC AGCTCTGTTT GCAACAAGAT CCTGAGAAAA 1141 GGCCATCAGC AAGCAGTTTA TTGTCCCATG TTTTCTTCAA ACAGATGAAA GAAGAAAGCC 1201 AGGATTCAAT ACTTTCACTG TTGCCTCCTG CTTATAACAA GCCATCAATA TCATTGCCTC 1261 CAGTGTTACC TTGGACTGAG CCAGAATGTG ATTTTCCTGA TGAAAAAGAC TCATACTGGG 1321 AATTCTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1381 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1441 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGGGCTC AGCGAAGGCA CAAATGGCAC 1501 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt