Construct: ORF TRCN0000488002
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020201.1_s317c1
- DNA Barcode:
- GCACTAATTCCGCCGAGACGGGCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PCK2 (5106)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488002
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5106 | PCK2 | phosphoenolpyruvate carboxy... | NM_004563.4 | 100% | 100% | |
| 2 | human | 5106 | PCK2 | phosphoenolpyruvate carboxy... | NM_001291556.2 | 79% | 79% | 0_1ins402 |
| 3 | human | 5106 | PCK2 | phosphoenolpyruvate carboxy... | NM_001308054.2 | 79% | 79% | 0_1ins402 |
| 4 | human | 5106 | PCK2 | phosphoenolpyruvate carboxy... | XM_006720158.3 | 77.4% | 76.5% | (many diffs) |
| 5 | human | 5106 | PCK2 | phosphoenolpyruvate carboxy... | NM_001018073.3 | 67.6% | 64.9% | (many diffs) |
| 6 | mouse | 74551 | Pck2 | phosphoenolpyruvate carboxy... | NM_028994.2 | 85.1% | 88.4% | (many diffs) |
| 7 | mouse | 74551 | Pck2 | phosphoenolpyruvate carboxy... | XM_006519632.2 | 71.8% | 73.1% | (many diffs) |
| 8 | mouse | 74551 | Pck2 | phosphoenolpyruvate carboxy... | XM_006519633.2 | 49.5% | 52.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1992
- ORF length:
- 1920
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccgca ttgtaccgcc ctggcctgcg gcttaactgg catgggctga 121 gccccttggg ctggccatca tgccgtagca tccagaccct gcgagtgctt agtggagatc 181 tgggccagct tcccactggc attcgagatt ttgtagagca cagtgcccgc ctgtgccaac 241 cagagggcat ccacatctgt gatggaactg aggctgagaa tactgccaca ctgaccctgc 301 tggagcagca gggcctcatc cgaaagctcc ccaagtacaa taactgctgg ctggcccgca 361 cagaccccaa ggatgtggca cgagtagaga gcaagacggt gattgtaact ccttctcagc 421 gggacacggt accactcccg cctggtgggg cccgtgggca gctgggcaac tggatgtccc 481 cagctgattt ccagcgagct gtggatgaga ggtttccagg ctgcatgcag ggccgcacca 541 tgtatgtgct tccattcagc atgggtcctg tgggctcccc gctgtcccgc atcggggtgc 601 agctcactga ctcagcctat gtggtggcaa gcatgcgtat tatgacccga ctggggacac 661 ctgtgcttca ggccctggga gatggtgact ttgtcaagtg tctgcactcc gtgggccagc 721 ccctgacagg acaaggggag ccagtgagcc agtggccgtg caacccagag aaaaccctga 781 ttggccacgt gcccgaccag cgggagatca tctccttcgg cagcggctat ggtggcaact 841 ccctgctggg caagaagtgc tttgccctac gcatcgcctc tcggctggcc cgggatgagg 901 gctggctggc agagcacatg ctgatcctgg gcatcaccag ccctgcaggg aagaagcgct 961 atgtggcagc cgccttccct agtgcctgtg gcaagaccaa cctggctatg atgcggcctg 1021 cactgccagg ctggaaagtg gagtgtgtgg gggatgatat tgcttggatg aggtttgaca 1081 gtgaaggtcg actccgggcc atcaaccctg agaacggctt ctttggggtt gcccctggta 1141 cctctgccac caccaatccc aacgccatgg ctacaatcca gagtaacact atttttacca 1201 atgtggctga gaccagtgat ggtggcgtgt actgggaggg cattgaccag cctcttccac 1261 ctggtgttac tgtgacctcc tggctgggca aaccctggaa acctggtgac aaggagccct 1321 gtgcacatcc caactctcga ttttgtgccc cggctcgcca gtgccccatc atggacccag 1381 cctgggaggc cccagagggt gtccccattg acgccatcat ctttggtggc cgcagaccca 1441 aaggggtacc cctggtatac gaggccttca actggcgtca tggggtgttt gtgggcagcg 1501 ccatgcgctc tgagtccact gctgcagcag aacacaaagg gaagatcatc atgcacgacc 1561 catttgccat gcggcccttt tttggctaca acttcgggca ctacctggaa cactggctga 1621 gcatggaagg gcgcaagggg gcccagctgc cccgtatctt ccatgtcaac tggttccggc 1681 gtgacgaggc agggcacttc ctgtggccag gctttgggga gaatgctcgg gtgctagact 1741 ggatctgccg gcggttagag ggggaggaca gtgcccgaga gacacccatt gggctggtgc 1801 caaaggaagg agccttggat ctcagcggcc TCAGAGCTAT AGACACCACT CAGCTGTTCT 1861 CCCTCCCCAA GGACTTCTGG GAACAGGAGG TTCGTGACAT TCGGAGCTAC CTGACAGAGC 1921 AGGTCAACCA GGATCTGCCC AAAGAGGTGT TGGCTGAGCT TGAGGCCCTG GAGAGACGTG 1981 TGCACAAAAT GTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2041 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2101 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCACTAATTC CGCCGAGACG 2161 GGCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt