Construct: ORF TRCN0000488018
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021262.1_s317c1
- DNA Barcode:
- TGTTATTATCCGGTGTACCCGTGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- N6AMT1 (29104)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488018
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29104 | N6AMT1 | N-6 adenine-specific DNA me... | NM_182749.4 | 99.8% | 100% | 108G>C |
| 2 | human | 29104 | N6AMT1 | N-6 adenine-specific DNA me... | NM_013240.6 | 86.7% | 86.9% | 108G>C;312_395del |
| 3 | human | 29104 | N6AMT1 | N-6 adenine-specific DNA me... | XR_001754831.2 | 46% | 1_22del;130G>C;581_1210del | |
| 4 | human | 29104 | N6AMT1 | N-6 adenine-specific DNA me... | NR_047510.2 | 42.4% | (many diffs) | |
| 5 | human | 29104 | N6AMT1 | N-6 adenine-specific DNA me... | XR_001754830.1 | 33% | (many diffs) | |
| 6 | human | 29104 | N6AMT1 | N-6 adenine-specific DNA me... | XR_002958591.1 | 5.4% | (many diffs) | |
| 7 | mouse | 67768 | N6amt1 | N-6 adenine-specific DNA me... | NM_026366.2 | 73.8% | 76.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 630
- ORF length:
- 558
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcaggg gagaacttcg ctacgccgtt ccacgggcac gtgggccgcg 121 gcgccttcag cgacgtgtac gagcccgcgg aggacacgtt tctgcttttg gacgcgctcg 181 aggcagcggc tgccgaactg gcaggagtgg aaatatgcct ggaagtaggg tcagggtctg 241 gtgtagtatc tgcattccta gcctctatga taggccctca ggctttgtac atgtgcactg 301 atatcaaccc TGAGGCAGCA GCTTGTACCC TAGAGACAGC ACGCTGTAAC AAAGTTCACA 361 TTCAACCAGT TATTACAGAT TTGGTAGGAA GTCACGGAAT AGAGGCAGCT TGGGCTGGTG 421 GCAGAAATGG TCGGGAAGTC ATGGACAGGT TTTTTCCCCT GGTTCCAGAT CTCCTTTCAC 481 CAAGAGGATT ATTCTATTTA GTTACCATTA AAGAAAACAA CCCAGAAGAA ATTTTGAAAA 541 TAATGAAGAC AAAAGGTCTG CAAGGAACCA CTGCACTTTC CAGACAAGCA GGCCAAGAAA 601 CTCTTTCAGT CCTCAAGTTC ACCAAGTCTT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 661 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 721 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 781 TTATTATCCG GTGTACCCGT GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 841 ttgtgaaaga tt