Transcript: Mouse NM_026366.2

Mus musculus N-6 adenine-specific DNA methyltransferase 1 (putative) (N6amt1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
N6amt1 (67768)
Length:
1791
CDS:
69..713

Additional Resources:

NCBI RefSeq record:
NM_026366.2
NBCI Gene record:
N6amt1 (67768)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_026366.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000039140 TGTTCTACTTAGTTACCGTAA pLKO.1 571 CDS 100% 4.050 5.670 N N6amt1 n/a
2 TRCN0000039139 GCCTTATACATGTGCACTGAT pLKO.1 279 CDS 100% 4.950 3.465 N N6amt1 n/a
3 TRCN0000039142 CCTCAGGTTCAGCAAGTCCTA pLKO.1 692 CDS 100% 2.640 1.848 N N6amt1 n/a
4 TRCN0000039141 GCCTTGAAGTAGGAGCAGGAT pLKO.1 214 CDS 100% 2.640 1.848 N N6amt1 n/a
5 TRCN0000039143 AGAGGTAGGAAGTCGTGGAAT pLKO.1 461 CDS 100% 4.950 2.970 N N6amt1 n/a
6 TRCN0000182716 CCTGAGTTCAATTCCCAGCAA pLKO.1 1509 3UTR 100% 2.640 1.320 Y BC028528 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_026366.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488018 TGTTATTATCCGGTGTACCCGTGT pLX_317 56.7% 73.8% 76.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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