Transcript: Human NM_182749.4

Homo sapiens N-6 adenine-specific DNA methyltransferase 1 (N6AMT1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-21
Taxon:
Homo sapiens (human)
Gene:
N6AMT1 (29104)
Length:
926
CDS:
29..589

Additional Resources:

NCBI RefSeq record:
NM_182749.4
NBCI Gene record:
N6AMT1 (29104)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_182749.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000427908 AGTCAGGCCTATAGAATTATT pLKO_005 728 3UTR 100% 15.000 12.000 N N6AMT1 n/a
2 TRCN0000038755 CCTTTCACCAAGAGGATTATT pLKO.1 430 CDS 100% 15.000 10.500 N N6AMT1 n/a
3 TRCN0000038758 GTTCACATTCAACCAGTTATT pLKO.1 311 CDS 100% 13.200 9.240 N N6AMT1 n/a
4 TRCN0000434624 GTACATGTGCACTGATATCAA pLKO_005 244 CDS 100% 5.625 3.938 N N6AMT1 n/a
5 TRCN0000038754 CGCTGTAACAAAGTTCACATT pLKO.1 299 CDS 100% 4.950 3.465 N N6AMT1 n/a
6 TRCN0000038757 AGAAACTCTTTCAGTCCTCAA pLKO.1 553 CDS 100% 4.050 2.835 N N6AMT1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_182749.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488018 TGTTATTATCCGGTGTACCCGTGT pLX_317 56.7% 99.8% 100% V5 (not translated due to prior stop codon) 108G>C n/a
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