Construct: ORF TRCN0000488020
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020441.1_s317c1
- DNA Barcode:
- GCGATGGCTAGATTTTCGAGTCCA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPK4 (5596)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488020
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5596 | MAPK4 | mitogen-activated protein k... | NM_002747.4 | 95% | 94.5% | 1145_1146delCG;1162delC;1677_1761del |
| 2 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_005258299.3 | 95% | 94.5% | 1145_1146delCG;1162delC;1677_1761del |
| 3 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_011526074.2 | 95% | 94.5% | 1145_1146delCG;1162delC;1677_1761del |
| 4 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_011526075.3 | 95% | 94.5% | 1145_1146delCG;1162delC;1677_1761del |
| 5 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_011526076.2 | 95% | 94.5% | 1145_1146delCG;1162delC;1677_1761del |
| 6 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_017025839.2 | 95% | 94.5% | 1145_1146delCG;1162delC;1677_1761del |
| 7 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_011526077.2 | 64.7% | 63.5% | (many diffs) |
| 8 | human | 5596 | MAPK4 | mitogen-activated protein k... | NM_001292039.2 | 59% | 58.6% | (many diffs) |
| 9 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_011526078.2 | 51.1% | 45.9% | (many diffs) |
| 10 | human | 5596 | MAPK4 | mitogen-activated protein k... | NM_001292040.2 | 41.6% | 41.3% | 693_694delTAinsGG;697_698insA;699_700ins973 |
| 11 | mouse | 225724 | Mapk4 | mitogen-activated protein k... | NM_172632.2 | 82.5% | 88.4% | (many diffs) |
| 12 | mouse | 225724 | Mapk4 | mitogen-activated protein k... | XM_006525900.3 | 82.5% | 88.4% | (many diffs) |
| 13 | mouse | 225724 | Mapk4 | mitogen-activated protein k... | XM_006525901.3 | 82.5% | 88.4% | (many diffs) |
| 14 | mouse | 225724 | Mapk4 | mitogen-activated protein k... | XM_017317891.1 | 55.3% | 58.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1746
- ORF length:
- 1674
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctgag aagggtgact gcatcgccag tgtctatggg tatgacctcg 121 gtgggcgctt tgttgacttc caacccctgg gcttcggtgt caatggtttg gtgctgtcgg 181 ccgtggacag ccgggcctgc cggaaggtcg ctgtgaagaa gattgccctg agcgatgccc 241 gcagcatgaa gcacgcgctc cgagagatca agatcattcg gcgcctggac cacgacaaca 301 tcgtcaaagt gtacgaggtg ctcggtccca agggcactga cctgcagggt gagctgttca 361 agttcagcgt ggcgtacatc gtccaggagt acatggagac cgacctggca cgcctgctgg 421 agcagggcac gctggcagaa gagcatgcca agctgttcat gtaccagctg ctccgcgggc 481 tcaagtacat ccactccgcc aacgtgctgc acagggacct gaagcccgcc aacatcttca 541 tcagcacaga ggacctcgtg ctcaagattg gggatttcgg gttggcaagg atcgttgatc 601 agcattactc ccacaagggt tatctgtcag aagggttggt aacaaagtgg taccgttccc 661 cacgactgct cctttccccc aataactaca ccaaagccat cgacatgtgg gccgccggct 721 gcatcctggc tgagatgctt acggggagaa tgctctttgc tggggcccat gagctggagc 781 agatgcaact catcctggag accatccctg taatccggga ggaagacaag gacgagctgc 841 tcagggtgat gccttccttt gtcagcagca cctgggaggt gaagaggcct ctgcgcaagc 901 tgctccctga agtgaacagt gaagccatcg actttctgga gaagatcctg acctttaacc 961 ccatggatcg cctaacagct gagatggggc tgcaacaccc ctacatgagc ccatactcgt 1021 gccctgagga cgagcccacc tcacaacacc ccttccgcat tgaggatgag atcgacgaca 1081 tcgtgctgat ggccgctaac cagagccagc tgtccaactg ggacacgtgc agttccaggt 1141 accctgtgag cctgtcgtcg gacctggagt ggcggcctga ccggtgccag gacgccagcg 1201 aggtacagcg cgacccgcgc gggttcggcg cactggctga ggacgtgcag gtggacccgc 1261 gcaaggactc gcacagcagc tccgagcgct tcctagagca gtcgcactcg tccatggagc 1321 gcgccttcga ggccgactac gggcgctcct gcgactacaa ggtggggtcg ccgtcctacc 1381 tggacaagct gctgtggcgc gacaacaagc cgcaccacta ctcggagccc aagctcatcc 1441 tggacctgtc gcactggaag caggcggccG GCGCGCCCCC CACGGCCACG GGGCTGGCGG 1501 ACACGGGGGC GCGCGAGGAC GAGCCGGCCA GCCTCTTCCT GGAGATCGCG CAGTGGGTCA 1561 AGAGCACGCA GGGCGGCCCA GAGCACGCCA GCCCGCCCGC CGACGACCCC GAGCGCCGCT 1621 TGTCTGCCTC GCCCCCCGGC CGCCCGGCCC CGGTGGACGG CGGCGCCAGC CCCCAGTTCG 1681 ACCTGGACGT GTTCATCTCC CGCGCCCTGA AGCTCTGCAC CAAGCCCGAG GACCTGCCGG 1741 ACAATAGGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 1801 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 1861 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCGATGG CTAGATTTTC GAGTCCAACG 1921 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt