Construct: ORF TRCN0000488050
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021204.1_s317c1
- DNA Barcode:
- ACCCCCATTTCCCACGAATCATAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- WASF2 (10163)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488050
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10163 | WASF2 | WASP family member 2 | NM_006990.5 | 99.9% | 100% | 1239G>A |
| 2 | human | 10163 | WASF2 | WASP family member 2 | NM_001201404.3 | 55.8% | 55% | (many diffs) |
| 3 | mouse | 242687 | Wasf2 | WAS protein family, member 2 | NM_153423.6 | 87.6% | 91.2% | (many diffs) |
| 4 | mouse | 242687 | Wasf2 | WAS protein family, member 2 | XM_006538848.3 | 87.6% | 91.2% | (many diffs) |
| 5 | mouse | 242687 | Wasf2 | WAS protein family, member 2 | XM_006538849.3 | 87.6% | 91.2% | (many diffs) |
| 6 | mouse | 242687 | Wasf2 | WAS protein family, member 2 | XM_006538850.3 | 87.6% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1566
- ORF length:
- 1494
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgccgtta gtaacgagga acatcgagcc aaggcacctg tgccgtcaga 121 cgttgcctag cgttagaagc gagctggaat gcgtgaccaa catcaccctg gcaaatgtca 181 tccgacagct gggcagcctg agtaaatatg cagaggacat ttttggagag ctctttactc 241 aggcaaatac ctttgcctct cgggtaagct cccttgctga gagggtcgac cgactacagg 301 ttaaagtcac tcagctggat cccaaggaag aagaagtgtc actgcaagga atcaacaccc 361 gaaaagcctt cagaagttcc accattcaag accagaagct ttttgacaga aactctctcc 421 cagtgcctgt cttagaaaca tacaatacct gtgatactcc tccccctctc aacaatctta 481 ccccttacag ggacgatgga aaagaggcac tcaaattcta cacagaccct tcatacttct 541 ttgatctttg gaaggagaag atgctgcagg acaccaagga tatcatgaaa gagaagagaa 601 agcataggaa agaaaagaaa gataatccaa atcgagggaa tgtaaaccca cgtaaaatca 661 agacacgtaa ggaagagtgg gagaaaatga agatggggca agaatttgtg gagtccaaag 721 aaaagctggg gacttctggg tatccaccca ctttggtgta ccagaatggc agcattggct 781 gtgttgaaaa cgtggatgca agtagctatc cgccaccacc acagtcagac tctgcttctt 841 caccttctcc ttccttctcc gaggacaact tgcctcctcc accagcagaa ttcagttacc 901 cagtggacaa ccaaagagga tctggtttgg ctggacccaa aagatccagt gtggtcagcc 961 caagccatcc accaccagct cctcctctag gctctccacc aggccctaaa cccgggtttg 1021 ctccaccacc tgcccctccg ccacctccgc ctccaatgat aggcatccca cctccaccac 1081 cgcctgtagg atttgggtct ccagggacgc ctccaccacc ctcaccccca tctttcccac 1141 ctcaccctga ttttgctgcc cctccacctc ctcctccacc accagcagct gactacccaa 1201 ctctgccacc acctcccttg tcccagccaa caggaggagc acctcctcct ccccctcctc 1261 ctccTCCTCC GGGGCCCCCT CCTCCCCCTT TCACTGGTGC AGATGGCCAA CCTGCTATAC 1321 CACCACCGCT TTCTGATACC ACCAAGCCCA AGTCCTCCTT GCCTGCCGTG AGCGATGCCC 1381 GTAGCGACCT GCTTTCAGCC ATCCGTCAAG GTTTTCAGCT GCGCAGGGTT GAGGAGCAGC 1441 GGGAACAAGA GAAGCGGGAT GTTGTGGGCA ATGACGTGGC CACCATCTTG TCTCGTCGCA 1501 TTGCTGTTGA GTACAGTGAC TCAGAAGATG ACTCCTCTGA ATTTGATGAG GACGACTGGT 1561 CCGATTAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACCCCC ATTTCCCACG AATCATAGAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt