Construct: ORF TRCN0000488100
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019558.1_s317c1
- DNA Barcode:
- TATGTCATCCAAGTGGGACCAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LCK (3932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488100
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | NM_001042771.2 | 100% | 100% | |
| 2 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | NM_005356.5 | 100% | 100% | |
| 3 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | NM_001330468.2 | 89.9% | 89.7% | 631_632ins153 |
| 4 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | XM_024447046.1 | 89.7% | 89.7% | 186_359del |
| 5 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | XM_024447047.1 | 89.7% | 89.7% | 186_359del |
| 6 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | XM_011541453.3 | 80.7% | 80.5% | 186_359del;805_806ins153 |
| 7 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | NM_001162433.1 | 88.6% | 96.8% | (many diffs) |
| 8 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | NM_010693.3 | 88.6% | 96.8% | (many diffs) |
| 9 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | XM_006502818.3 | 88.6% | 96.8% | (many diffs) |
| 10 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | XM_030253275.1 | 88.6% | 96.8% | (many diffs) |
| 11 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | XM_030253276.1 | 88.6% | 96.8% | (many diffs) |
| 12 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | NM_001162432.1 | 86.7% | 94.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 1623
- ORF length:
- 1527
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgaa ttctgggcag ggaccatggg ctgtggctgc agctcacacc 121 cggaagatga ctggatggaa aacatcgatg tgtgtgagaa ctgccattat cccatagtcc 181 cactggatgg caagggcacg ctgctcatcc gaaatggctc tgaggtgcgg gacccactgg 241 ttacctacga aggctccaat ccgccggctt ccccactgca agacaacctg gttatcgctc 301 tgcacagcta tgagccctct cacgacggag atctgggctt tgagaagggg gaacagctcc 361 gcatcctgga gcagagcggc gagtggtgga aggcgcagtc cctgaccacg ggccaggaag 421 gcttcatccc cttcaatttt gtggccaaag cgaacagcct ggagcccgaa ccctggttct 481 tcaagaacct gagccgcaag gacgcggagc ggcagctcct ggcgcccggg aacactcacg 541 gctccttcct catccgggag agcgagagca ccgcgggatc gttttcactg tcggtccggg 601 acttcgacca gaaccaggga gaggtggtga aacattacaa gatccgtaat ctggacaacg 661 gtggcttcta catctcccct cgaatcactt ttcccggcct gcatgaactg gtccgccatt 721 acaccaatgc ttcagatggg ctgtgcacac ggttgagccg cccctgccag acccagaagc 781 cccagaagcc gtggtgggag gacgagtggg aggttcccag ggagacgctg aagctggtgg 841 agcggctggg ggctggacag ttcggggagg tgtggatggg gtactacaac gggcacacga 901 aggtggcggt gaagagcctg aagcagggca gcatgtcccc ggacgccttc ctggccgagg 961 ccaacctcat gaagcagctg caacaccagc ggctggttcg gctctacgct gtggtcaccc 1021 aggagcccat ctacatcatc actgaataca tggagaatgg gagtctagtg gattttctca 1081 agaccccttc aggcatcaag ttgaccatca acaaactcct ggacatggca gcccaaattg 1141 cagaaggcat ggcattcatt gaagagcgga attatattca tcgtgacctt cgggctgcca 1201 acattctggt gtctgacacc ctgagctgca agattgcaga ctttggccta gcacgcctca 1261 ttgaggacaa cgagtacaca gccagggagg gggccaagtt tcccattaag tggacagcgc 1321 cagaagccat taacTACGGG ACATTCACCA TCAAGTCAGA TGTGTGGTCT TTTGGGATCC 1381 TGCTGACGGA AATTGTCACC CACGGCCGCA TCCCTTACCC AGGGATGACC AACCCGGAGG 1441 TGATTCAGAA CCTGGAGCGA GGCTACCGCA TGGTGCGCCC TGACAACTGT CCAGAGGAGC 1501 TGTACCAACT CATGAGGCTG TGCTGGAAGG AGCGCCCAGA GGACCGGCCC ACCTTTGACT 1561 ACCTGCGCAG TGTGCTGGAG GACTTCTTCA CGGCCACAGA GGGCCAGTAC CAGCCTCAGC 1621 CTAAGGGTGG GCGCGCCGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1681 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1741 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATATGTCA TCCAAGTGGG 1801 ACCAATCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt