Construct: ORF TRCN0000488104
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021725.1_s317c1
- DNA Barcode:
- TATGTACGTCCGGGTACTGATTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EGFR (1956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488104
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346941.2 | 55.9% | 1_1243del;1560G>A;1908T>C | |
| 2 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346900.2 | 45.6% | 1_1885del;2202G>A;2550T>C | |
| 3 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346899.1 | 45.3% | 1_1909del;2226G>A;2574T>C | |
| 4 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.5 | 43.6% | 1_2044del;2361G>A;2709T>C | |
| 5 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2239_2247del | 43.4% | 1_2044del;2235_2236insGAATTAAGA;2352G>A | |
| 6 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2240_2254del | 43.2% | 1_2044del;2237_2238insATTAAGAGAAGCAAC;2346G>A | |
| 7 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346897.2 | 37.8% | (many diffs) | |
| 8 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346898.2 | 36.4% | (many diffs) | |
| 9 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_207655.2 | 37.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 304
- ORF end:
- 316
- ORF length:
- 12
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttgct gcaggagagg gagcttgtgg agcctcttac acccagtgga gaagctccca 121 accaagctct cttgaggatc ttgaaggaaa ctgaattcaa aaagatcaaa gtgctgggct 181 ccggtgcgtt cggcacggtg tataagggac tctggatccc agaaggtgag aaagttaaaa 241 ttcccgtcgc tatcaaggaa ttaagagaag caacatctcc gaaagccaac aaggaaatcc 301 tcgatgaagc ctacgtgatg gccagcgtgg acaaccccca cgtgtgccgc ctgctgggca 361 tctgcctcac ctccaccgtg caactcatca cgcagctcat gcccttcggc tgcctcctgg 421 actatgtccg ggaacacaaa gacaatattg gctcccagta cctgctcaac tggtgtgtgc 481 agatcgcaaa gggcatgaac tacttggagg accgtcgctt ggtgcaccgc gacctggcag 541 ccaggaacgt actggtgaaa acaccgcagc atgtcaagat cacagatttt gggctggcca 601 aactgctggg tgcggaagag aaagaatacc atgcagaagg aggcaaagtg cctatcaagt 661 ggatggcatt ggaatcaatt ttacacagaa tctataccca ccagagtgat gtctggagct 721 acggggtgac cgtttgggag ttgatgacct ttggatccaa gccatatgac ggaatccctg 781 ccagcgagat ctcctccatc ctggagaaag gagaacgcct ccctcagcca cccatatgta 841 ccatcgatgt ctacatgatc atggtcaagt gctggatgat agacgcagat agtcgcccaa 901 agttccgtga gttgatcatc gaattctcca aaatggcccg agacccccag cgctaccttg 961 tcattcaggg ggatgaaaga atgcatttgc caagtcctac agactccaac ttctaccgtg 1021 ccctgatgga tgaagaagac atggacgacg tggtggatgc cgacgagtac ctcatcccac 1081 agcagggctt cttcagcagc ccctccacgt cacggactcc cctcctgagc tctctgagtg 1141 caaccagcaa caattccacc gtggcttgca ttgatagaaa tgggctgcaa agctgtccca 1201 tcaaggaaga cagcttcttg cagcgataca gctcagaccc cacaggcgcc ttgactgagg 1261 acagcataga cgacaccttc ctcccagtgc ctgaatacat aaaccagtcc gttcccaaaa 1321 ggcccgctGG CTCTGTGCAG AATCCTGTCT ATCACAATCA GCCTCTGAAC CCCGCGCCCA 1381 GCAGAGACCC ACACTACCAG GACCCCCACA GCACTGCAGT GGGCAACCCC GAGTATCTCA 1441 ACACTGTCCA GCCCACCTGT GTCAACAGCA CATTCGACAG CCCTGCCCAC TGGGCCCAGA 1501 AAGGCAGCCA CCAAATTAGC CTGGACAACC CTGACTACCA GCAGGACTTC TTTCCCAAGG 1561 AAGCCAAGCC AAATGGCATC TTTAAGGGCT CCACAGCTGA AAATGCAGAA TACCTAAGGG 1621 TCGCGCCACA AAGCAGTGAA TTTATTGGAG CATGAGACCC AGCTTTCTTG TACAAAGTGG 1681 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1741 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1801 ATATGTACGT CCGGGTACTG ATTCCACGCG TTAAGTCgac aatcaacctc tggattacaa 1861 aatttgtgaa agatt