Construct: ORF TRCN0000488105

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021524.1_s317c1
DNA Barcode:
AGTGCTTGTTGTCAAGCATAGGAG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
USP54 (159195)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488105
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 159195 USP54 ubiquitin specific peptidas... XM_005269582.2 45.5% 45.5% 1_2565del;3985_3999del
2 human 159195 USP54 ubiquitin specific peptidas... NM_001350995.2 45.3% 45.3% 1_2445del;3865_4020del
3 human 159195 USP54 ubiquitin specific peptidas... XM_024447852.1 45.3% 45.3% 1_2445del;3865_4020del
4 human 159195 USP54 ubiquitin specific peptidas... XM_024447851.1 44.4% 44.4% 1_2682del;4102_4116del
5 human 159195 USP54 ubiquitin specific peptidas... NM_001320437.2 44.3% 44.3% 1_2565del;3985_4125del
6 human 159195 USP54 ubiquitin specific peptidas... XM_017015783.1 44.2% 44.2% 1_2565del;3985_4140del
7 human 159195 USP54 ubiquitin specific peptidas... XM_017015784.1 44.2% 44.2% 1_2565del;3985_4140del
8 human 159195 USP54 ubiquitin specific peptidas... XM_017015782.2 43.7% 43.7% 1_2616del;4036_4191del
9 human 159195 USP54 ubiquitin specific peptidas... XM_011539368.2 43.6% 43.6% 1_2631del;4051_4206del
10 human 159195 USP54 ubiquitin specific peptidas... XM_024447850.1 43.5% 43.5% 1_2802del
11 human 159195 USP54 ubiquitin specific peptidas... XM_024447849.1 43.3% 43.3% 1_2802del;4222_4236del
12 human 159195 USP54 ubiquitin specific peptidas... XM_024447848.1 43.2% 43.2% 1_2682del;4102_4257del
13 human 159195 USP54 ubiquitin specific peptidas... NM_152586.3 42.7% 42.7% 1_2736del;4156_4311del
14 human 159195 USP54 ubiquitin specific peptidas... XM_017015774.1 42.7% 42.7% 1_2736del;4156_4311del
15 human 159195 USP54 ubiquitin specific peptidas... XM_017015775.1 42.7% 42.7% 1_2736del;4156_4311del
16 human 159195 USP54 ubiquitin specific peptidas... XM_017015777.1 42.7% 42.7% 1_2736del;4156_4311del
17 human 159195 USP54 ubiquitin specific peptidas... XM_024447847.1 42.7% 42.7% 1_2736del;4156_4311del
18 human 159195 USP54 ubiquitin specific peptidas... XM_024447846.1 42.3% 42.3% 1_2802del;4222_4362del
19 human 159195 USP54 ubiquitin specific peptidas... XM_024447832.1 42.2% 42.2% 1_2802del;4222_4377del
20 human 159195 USP54 ubiquitin specific peptidas... XM_024447833.1 42.2% 42.2% 1_2802del;4222_4377del
21 human 159195 USP54 ubiquitin specific peptidas... XM_024447834.1 42.2% 42.2% 1_2802del;4222_4377del
22 human 159195 USP54 ubiquitin specific peptidas... XM_024447835.1 42.2% 42.2% 1_2802del;4222_4377del
23 human 159195 USP54 ubiquitin specific peptidas... XM_024447836.1 42.2% 42.2% 1_2802del;4222_4377del
24 human 159195 USP54 ubiquitin specific peptidas... XM_024447837.1 42.2% 42.2% 1_2802del;4222_4377del
25 human 159195 USP54 ubiquitin specific peptidas... XM_024447838.1 42.2% 42.2% 1_2802del;4222_4377del
26 human 159195 USP54 ubiquitin specific peptidas... XM_024447839.1 42.2% 42.2% 1_2802del;4222_4377del
27 human 159195 USP54 ubiquitin specific peptidas... XM_024447840.1 42.2% 42.2% 1_2802del;4222_4377del
28 human 159195 USP54 ubiquitin specific peptidas... XM_024447841.1 42.2% 42.2% 1_2802del;4222_4377del
29 human 159195 USP54 ubiquitin specific peptidas... XM_024447842.1 42.2% 42.2% 1_2802del;4222_4377del
30 human 159195 USP54 ubiquitin specific peptidas... XM_024447843.1 42.2% 42.2% 1_2802del;4222_4377del
31 human 159195 USP54 ubiquitin specific peptidas... XM_024447844.1 42.2% 42.2% 1_2802del;4222_4377del
32 human 159195 USP54 ubiquitin specific peptidas... XM_024447845.1 42.2% 42.2% 1_2802del;4222_4377del
33 human 159195 USP54 ubiquitin specific peptidas... NR_135250.1 38.7% 1_2135del;3555_3695del;4437_5569del
34 human 159195 USP54 ubiquitin specific peptidas... XR_001747048.1 37.6% 1_2331del;3751_3906del;4648_5741del
35 human 159195 USP54 ubiquitin specific peptidas... XR_001747042.1 36.5% 1_2502del;3922_4077del;4819_5912del
36 human 159195 USP54 ubiquitin specific peptidas... NR_146998.2 36% 1_2728del;4148_4162del;4904_5997del
37 human 159195 USP54 ubiquitin specific peptidas... NR_146997.2 35.1% 1_2728del;4148_4303del;5045_6138del
38 human 159195 USP54 ubiquitin specific peptidas... NR_135249.2 33.7% 1_3132del;4552_4566del;5308_6401del
39 human 159195 USP54 ubiquitin specific peptidas... XR_001747041.2 31.6% 1_3407del;4827_4982del;5724_6817del
40 human 159195 USP54 ubiquitin specific peptidas... XR_001747037.2 28.7% 1_4093del;5513_5668del;6410_7503del
41 human 159195 USP54 ubiquitin specific peptidas... XR_001747046.1 27.7% 1_4385del;5805_5960del;6702_7795del
42 human 159195 USP54 ubiquitin specific peptidas... XR_001747043.1 27.6% 1_4556del;5976_5990del;6732_7825del
43 human 159195 USP54 ubiquitin specific peptidas... XR_001747035.1 26.8% 1_4622del;6042_6197del;6939_8032del
44 human 159195 USP54 ubiquitin specific peptidas... XR_001747038.1 25.5% 1_5055del;6475_6630del;7372_8465del
45 human 159195 USP54 ubiquitin specific peptidas... XR_001747047.1 24.2% 1_5515del;6935_7075del;7817_8910del
46 human 159195 USP54 ubiquitin specific peptidas... XR_001747044.1 24.1% 1_5686del;7847_8940del
47 human 159195 USP54 ubiquitin specific peptidas... XR_001747039.1 24.1% 1_5686del;7106_7120del;7862_8955del
48 human 159195 USP54 ubiquitin specific peptidas... XR_002956962.1 24% 1_5581del;7001_7156del;7898_8991del
49 human 159195 USP54 ubiquitin specific peptidas... XR_001747036.1 23.9% 1_5752del;7172_7186del;7928_9021del
50 human 159195 USP54 ubiquitin specific peptidas... XR_002956959.1 23.5% 1_5752del;7172_7327del;8069_9162del
51 human 159195 USP54 ubiquitin specific peptidas... XR_002956960.1 20.6% 1_5752del;7386_7387ins526
52 human 159195 USP54 ubiquitin specific peptidas... XR_002956961.1 18% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2229
ORF length:
2160
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat ggatacagag tttggggcca gttctttctt ccattcacct gcttcctgcc
121atgagtcaca ctcatcacta tctccagagt catctgcccc acagcacagc tcccccagta
181gatctgcctt gaagcttctg acttcggttg aagtagacaa cattgaaccc tctgcattcc
241acaggcaagg tttacctaaa gcaccagggt ggactgagaa gaattctcat catagttggg
301agccattgga tgccccagag ggtaagctgc aaggctctag gtgtgacaac agcagttgca
361gcaagctccc tccacaagaa ggaagaggca ttgctcaaga acagctgttc caagaaaaga
421aggatcctgc taacccctcc ccggtgatgc ctggaatagc cacctctgag aggggtgatg
481aacacagcct aggctgtagt ccttcaaatt catcagctca gcccagcctt cccctgtata
541gaacctgcca ccccataatg cctgttgctt cttcatttgt gcttcactgt cctgatcctg
601tgcagaaaac taaccaatgc ctccaaggcc aaagcctcaa aacttcattg actttaaaag
661tggacagagg cagtgaggag acctataggc cagagtttcc cagcacaaag gggcttgtcc
721gttctctggc tgagcagttc cagaggatgc agggtgtctc catgagggat agtacaggtt
781tcaaggatag aagtttgtca ggtagtctaa ggaagaactc ttccccttct gattctaagc
841ctcctttctc acagggtcaa gagaaaggcc actggccatg ggcaaagcaa caatcctctc
901tggagggtgg ggatagacca ctttcctggg aagagtccac tgaacattct tctcttgcct
961taaactctgg gctgcctaat ggtgaaactt ctagcggagg acagcccagg ttggcagagc
1021cagacatata ccaagagaag ctgtcccaag tgagagatgt taggtctaag gatctgggca
1081gcagtactga cttggggact tccttgcctt tggattcctg ggtgaatatc acaaggttct
1141gtgattctca gcttaagcat ggggcaccta ggccaggaat gaagtcctcc cctcatgatt
1201cccatacgtg tgtaacctat ccagagagaa atcacatcct tttgcatcca cattggaacc
1261aagacacaga gcaggagacc tcagaattgg agtctctgta tcaggccagt cttcaggctt
1321ctcaagctgg ctgttctgga tgggggcagc aggataccgc ctggcaccca cttagccaaa
1381caggctctgc agatggcatg gggaggaggt tgcactcagc ccatgatcct ggtctctcaa
1441agacttcaac agcagaaatg gagcatggtc tccatgaagc cagaacagat tcatcaaacg
1501tgaggaagcc tttggaaacc gggcaccgtt gttccagctc ctcttccctc cctgtcatcc
1561atgacccttc tgtgtttctc ctcggtcccc aactctacct tccccaacca cagttcctgt
1621ccccagatgt cctgatgccc accatggcag gggagcccaa tagactccca ggaacttcaa
1681ggagtgtcca gcagtttctg gctatgtgtg acaggggtga aacttcccaa ggggccaagt
1741acacaggaag gactttgaac taccagagcc tcccccatcg ctccagaaca gacaactcct
1801gggcaccctg gtcagagacc aaccagcata ttgggaccag attcctgact actccagggt
1861gcaatcctca actaacctac actgccacac taccagaaag aagcaagggc cttcaggttc
1921ctcacactca gtcctGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC ATTGTTCATC
1981CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG AATTCAGATC
2041CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA GATGATGACT
2101GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA GAGGGGTTTC
2161TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC CTGCAGCACA
2221GTCAATGGTA AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
2281ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
2341CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGT GCTTGTTGTC AAGCATAGGA
2401GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)