Construct: ORF TRCN0000488156
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021211.1_s317c1
- DNA Barcode:
- ATCAAAAGTCGAGTTTGTGCACTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CERK (64781)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488156
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64781 | CERK | ceramide kinase | NM_022766.6 | 99.9% | 100% | 1164T>C |
| 2 | human | 64781 | CERK | ceramide kinase | XM_017028909.1 | 92.3% | 91.6% | (many diffs) |
| 3 | mouse | 223753 | Cerk | ceramide kinase | XM_006520831.3 | 51.2% | 53.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1683
- ORF length:
- 1611
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggggcg acgggggcgg cggagccgct gcaatccgtg ctgtgggtga 121 agcagcagcg ctgcgccgtg agcctggagc ccgcgcgggc tctgctgcgc tggtggcgga 181 gcccggggcc cggagccggc gcccccggcg cggatgcctg ctctgtgcct gtatctgaga 241 tcatcgccgt tgaggaaaca gacgttcacg ggaaacatca aggcagtgga aaatggcaga 301 aaatggaaaa gccttacgct tttacagttc actgtgtaaa gagagcacga cggcaccgct 361 ggaagtgggc gcaggtgact ttctggtgtc cagaggagca gctgtgtcac ttgtggctgc 421 agaccctgcg ggagatgctg gagaagctga cgtccagacc aaagcattta ctggtattta 481 tcaacccgtt tggaggaaaa ggacaaggca agcggatata tgaaagaaaa gtggcaccac 541 tgttcacctt agcctccatc accactgaca tcatcgttac tgaacatgct aatcaggcca 601 aggagactct gtatgagatt aacatagaca aatacgacgg catcgtctgt gtcggcggag 661 atggtatgtt cagcgaggtg ctgcacggtc tgattgggag gacgcagagg agcgccgggg 721 tcgaccagaa ccacccccgg gctgtgctgg tccccagtag cctccggatt ggaatcattc 781 ccgcagggtc aacggactgc gtgtgttact ccaccgtggg caccagcgac gcagaaacct 841 cggcgctgca tatcgttgtt ggggactcgc tggccatgga tgtgtcctca gtccaccaca 901 acagcacact ccttcgctac tccgtgtccc tgctgggcta cggcttctac ggggacatca 961 tcaaggacag tgagaagaaa cggtggttgg gtcttgccag atacgacttt tcaggtttaa 1021 agaccttcct ctcccaccac tgctatgaag ggacagtgtc cttcctccct gcacaacaca 1081 cggtgggatc tccaagggat aggaagccct gccgggcagg atgctttgtt tgcaggcaaa 1141 gcaagcagca gctggaggag gagcagaaga aagcactgta tggtttggaa gctgcggagg 1201 acgtggagga gtggcaagtc gtctgtggga agttcctggc catcaatgcc acaaacatgt 1261 cctgtgcttg tcgccggagc cccaggggcc tctccccggc tgcccacttg ggagacgggt 1321 cttctgacct catcctcatc cggaaatgct ccaggttcaa tttTCTGAGA TTTCTCATCA 1381 GGCACACCAA CCAGCAGGAC CAGTTTGACT TCACTTTTGT TGAAGTTTAT CGCGTCAAGA 1441 AATTCCAGTT TACGTCGAAG CACATGGAGG ATGAGGACAG CGACCTCAAG GAGGGGGGGA 1501 AGAAGCGCTT TGGGCACATT TGCAGCAGCC ACCCCTCCTG CTGCTGCACC GTCTCCAACA 1561 GCTCCTGGAA CTGCGACGGG GAGGTCCTGC ACAGCCCTGC CATCGAGGTC AGAGTCCACT 1621 GCCAGCTGGT TCGACTCTTT GCACGAGGAA TTGAAGAGAA TCCGAAGCCA GACTCACACA 1681 GCTGAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1741 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1801 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCAAAAGT CGAGTTTGTG CACTGACGCG 1861 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt